##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547945_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3671365
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37826394270251	31.0	31.0	33.0	30.0	34.0
2	31.77594545897779	31.0	31.0	34.0	30.0	34.0
3	31.807945546138836	31.0	31.0	34.0	30.0	34.0
4	35.626376565664266	37.0	35.0	37.0	33.0	37.0
5	35.56809361095941	37.0	35.0	37.0	33.0	37.0
6	35.63300352866032	37.0	35.0	37.0	33.0	37.0
7	35.94988349020051	37.0	35.0	37.0	35.0	37.0
8	35.964471524896055	37.0	35.0	37.0	35.0	37.0
9	37.75124156819058	39.0	38.0	39.0	35.0	39.0
10	37.21270971423435	39.0	37.0	39.0	33.0	39.0
11	36.9347812053555	39.0	37.0	39.0	33.0	39.0
12	36.45778341298128	39.0	35.0	39.0	32.0	39.0
13	36.295222076802496	39.0	35.0	39.0	32.0	39.0
14	37.303538874505804	40.0	35.0	41.0	32.0	41.0
15	37.514896503071746	40.0	35.0	41.0	32.0	41.0
16	37.61760516864981	40.0	35.0	41.0	33.0	41.0
17	37.55543619335043	39.0	35.0	41.0	33.0	41.0
18	37.46423006157111	39.0	36.0	41.0	32.0	41.0
19	37.37090455457302	39.0	36.0	41.0	32.0	41.0
20	37.21118739215523	39.0	35.0	41.0	32.0	41.0
21	37.10735925194035	39.0	35.0	41.0	32.0	41.0
22	37.04416804104196	39.0	35.0	41.0	32.0	41.0
23	36.92953928579697	39.0	35.0	41.0	32.0	41.0
24	36.877573600009804	38.0	35.0	41.0	32.0	41.0
25	36.88291956806256	38.0	35.0	41.0	32.0	41.0
26	36.79375109802485	38.0	35.0	41.0	31.0	41.0
27	36.71109410260217	38.0	35.0	41.0	31.0	41.0
28	36.678275518778435	38.0	35.0	41.0	31.0	41.0
29	36.66945536605595	38.0	35.0	41.0	31.0	41.0
30	36.5990322400524	38.0	35.0	41.0	31.0	41.0
31	36.43787664805869	38.0	35.0	40.0	31.0	41.0
32	36.26064801511155	38.0	35.0	40.0	30.0	41.0
33	36.118732950823464	38.0	35.0	41.0	30.0	41.0
34	35.94961764902155	38.0	35.0	40.0	30.0	41.0
35	35.84232540213245	38.0	35.0	41.0	29.0	41.0
36	35.75035742836792	38.0	35.0	40.0	29.0	41.0
37	35.6324013003338	38.0	35.0	40.0	28.0	41.0
38	35.516760115107054	38.0	35.0	40.0	27.0	41.0
39	35.46604627978967	38.0	35.0	40.0	27.0	41.0
40	35.32049714479492	38.0	34.0	40.0	26.0	41.0
41	35.238828065310855	38.0	34.0	40.0	26.0	41.0
42	35.23483064200917	38.0	34.0	40.0	26.0	41.0
43	35.17500357496463	38.0	34.0	40.0	26.0	41.0
44	35.06038816625424	38.0	34.0	40.0	26.0	41.0
45	34.95329175933202	38.0	34.0	40.0	25.0	41.0
46	34.90371891653377	37.0	34.0	40.0	25.0	41.0
47	34.827164555962156	37.0	34.0	40.0	25.0	41.0
48	34.705603501694874	37.0	34.0	40.0	24.0	41.0
49	34.57727384773783	37.0	34.0	40.0	24.0	41.0
50	34.48236337166149	37.0	34.0	40.0	24.0	41.0
51	34.18846287416261	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	4.0
12	6.0
13	26.0
14	25.0
15	90.0
16	211.0
17	558.0
18	1252.0
19	2441.0
20	4380.0
21	7349.0
22	11530.0
23	17509.0
24	26714.0
25	40248.0
26	53797.0
27	61097.0
28	66138.0
29	73221.0
30	86659.0
31	104331.0
32	130597.0
33	170163.0
34	278490.0
35	391419.0
36	281327.0
37	349421.0
38	544554.0
39	967651.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.549863606587742	25.406572214966367	30.164747988827045	13.878816189618847
2	32.39446363954551	25.9162464097141	28.40916117030042	13.280128780439973
3	28.498092671254426	25.66244434971734	32.718021771194095	13.121441207834145
4	25.022137542848505	27.491219205935668	32.58736192124728	14.899281329968552
5	22.50626129518585	32.33715525424468	30.759812767186045	14.396770683383428
6	21.3348441247329	40.72842117305144	27.588376530255093	10.348358171960564
7	88.7435327187572	2.7836785500760617	7.06758930261633	1.4051994285504166
8	89.33769864886766	2.1901935656084315	6.838192334458709	1.6339154510652032
9	84.50355657909252	5.074243503438095	8.101046885831293	2.3211530316380964
10	39.699539544556316	37.82140974814545	13.484929991978461	8.994120715319779
11	31.82380395302565	24.338794971352616	27.60676206261159	16.230639013010148
12	30.69741090847682	23.390973112180347	28.705753854492812	17.20586212485002
13	23.493087720779602	30.635199714547586	29.41186724828504	16.459845316387774
14	16.933946910753903	34.36294130384748	30.678017576568934	18.025094208829685
15	16.014670292929196	28.725283375529266	38.74076807944729	16.51927825209425
16	20.39459438110893	26.65934877082502	36.76267546266851	16.18338138539753
17	20.15337619659173	25.49422353811185	29.566033341822457	24.786366923473967
18	21.743465986084196	26.846908438686974	32.412740220599154	18.99688535462968
19	23.710145953889082	28.62444894473854	28.851912027270508	18.813493074101867
20	24.879002768724984	28.881982586858022	28.464099864764197	17.7749147796528
21	22.31995456730671	28.452551026661745	31.12648837693882	18.101006029092723
22	22.13658952460461	25.209561021581887	30.66979175320351	21.98405770060999
23	18.25174015659026	29.650661266313755	30.624331822087967	21.473266755008012
24	18.826512754792834	28.605028375004938	33.98588808249793	18.5825707877043
25	19.219609055487535	29.17470749979912	31.870544061949712	19.73513938276363
26	18.693837305743234	32.6217632951232	28.298439408775756	20.38595999035781
27	18.026565051418206	32.06978875704268	31.09257728392573	18.811068907613382
28	16.319733940918432	30.684772557345834	33.91697638344321	19.078517118292517
29	17.459228379635366	28.216671455984354	33.669575212489086	20.65452495189119
30	19.53159111120796	29.110616895895667	32.30030247605455	19.057489516841827
31	23.71469467078321	28.383339711524187	28.480878365403605	19.421087252289
32	23.462390691200685	29.016891537616118	29.511557690395808	18.00916008078739
33	22.72901223386942	28.64700186442917	27.976215930587124	20.647769971114286
34	18.832559552101195	29.83560610290723	30.271710930403266	21.060123414588308
35	18.21453328666586	30.04765257608546	30.421219355743705	21.31659478150497
36	23.863113583095117	28.306311140406905	28.194309201073715	19.636266075424263
37	19.348035403725863	31.774884818044512	29.325659529902364	19.551420248327254
38	19.465212529944584	32.00752308746202	27.408470691418586	21.118793691174808
39	19.005764885812226	30.39893881431021	28.480633224972184	22.114663074905383
40	21.381774898436955	28.869698327461315	28.397231002637984	21.351295771463747
41	17.903313890065412	27.71753829978768	29.78475308230045	24.594394727846456
42	20.565348310505765	28.023528033851168	28.54570439060132	22.865419265041748
43	20.343877549630722	28.416297480637308	28.757369534219563	22.482455435512406
44	20.14485075714346	30.187518811123383	28.34591493899408	21.321715492739077
45	18.56413077969638	32.204534280846495	26.825336080722018	22.405998858735103
46	21.088586942458733	30.634110201519054	27.66148285446966	20.615820001552557
47	19.61714512177351	29.982554172630614	29.2061399506723	21.194160754923576
48	20.458821174140954	29.30811837014298	29.410968400036495	20.822092055679565
49	21.01673355822698	26.966863823128456	29.912716387501654	22.103686231142913
50	18.935327868517568	30.14655312125054	29.454603396829242	21.463515613402645
51	18.092671254424445	30.91272592074065	27.44861924652003	23.54598357831488
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1758.0
1	3341.5
2	4925.0
3	27165.0
4	49405.0
5	34757.0
6	20109.0
7	19237.5
8	18366.0
9	20488.0
10	22610.0
11	25014.0
12	27418.0
13	27609.5
14	27801.0
15	25952.5
16	24104.0
17	22305.5
18	20507.0
19	19928.5
20	19350.0
21	18544.0
22	17738.0
23	20604.5
24	23471.0
25	27537.5
26	37957.5
27	44311.0
28	55031.0
29	65751.0
30	74721.5
31	83692.0
32	97180.5
33	110669.0
34	120604.0
35	130539.0
36	138800.5
37	147062.0
38	167833.5
39	188605.0
40	212152.0
41	235699.0
42	258921.5
43	282144.0
44	295633.0
45	309122.0
46	314345.5
47	319569.0
48	318280.5
49	316992.0
50	294788.0
51	272584.0
52	248337.5
53	224091.0
54	198006.5
55	171922.0
56	154522.5
57	137123.0
58	122134.5
59	107146.0
60	94098.5
61	81051.0
62	68374.5
63	55698.0
64	45045.0
65	34392.0
66	26817.0
67	19242.0
68	14889.5
69	10537.0
70	8312.0
71	6087.0
72	4558.0
73	3029.0
74	2845.0
75	2026.5
76	1392.0
77	955.0
78	518.0
79	435.0
80	352.0
81	227.0
82	102.0
83	73.0
84	44.0
85	36.5
86	29.0
87	24.5
88	20.0
89	13.0
90	6.0
91	7.5
92	9.0
93	7.0
94	5.0
95	4.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3671365.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.929673797619113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.18247239034606	14.983621500381023
2	9.454617897658506	3.7685490116293097
3	3.683824047044456	2.202522347562633
4	1.9543210492508882	1.557959240295637
5	1.1930079440297585	1.1888129581240663
6	0.8388760978777259	1.0031116192393608
7	0.5851091367718184	0.8162723962308194
8	0.426048778909178	0.6792810548427091
9	0.36362599069012774	0.6522252640911297
>10	3.80537531106166	17.643048872784224
>50	1.437589090765771	20.68968787293555
>100	1.066503954278059	32.22686297030845
>500	0.005844985085056906	0.7752319397764212
>1k	0.0026441599194305053	0.8565521089089912
>5k	0.0	0.0
>10k+	1.3916631154897396E-4	0.9562608428897517
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34478	0.9391057549440059	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4291	0.1168775101358759	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.171347713997382E-5	0.0	0.0	0.06463536041771929	0.0
2	8.171347713997382E-5	0.0	0.0	0.19741976077017676	0.0
3	8.171347713997382E-5	0.0	0.0	0.321542532545797	0.0
4	8.171347713997382E-5	0.0	0.0	0.486930610277104	0.0
5	8.171347713997382E-5	0.0	0.0	0.9166617865562263	0.0
6	8.171347713997382E-5	0.0	0.0	1.429822422995262	0.0
7	8.171347713997382E-5	0.0	0.0	1.7899881924025534	0.0
8	8.171347713997382E-5	0.0	0.0	2.476326924726907	0.0
9	8.171347713997382E-5	0.0	2.723782571332461E-5	2.812087602295059	0.0
10	8.171347713997382E-5	0.0	2.723782571332461E-5	3.3041661616319815	0.0
11	8.171347713997382E-5	0.0	2.723782571332461E-5	3.8788570463574175	0.0
12	8.171347713997382E-5	0.0	2.723782571332461E-5	4.298564702774036	0.0
13	8.171347713997382E-5	0.0	2.723782571332461E-5	4.472832311687887	0.0
14	8.171347713997382E-5	0.0	2.723782571332461E-5	4.558658700510573	0.0
15	8.171347713997382E-5	0.0	2.723782571332461E-5	4.66308852429546	0.0
16	8.171347713997382E-5	0.0	2.723782571332461E-5	4.875679753987958	0.0
17	8.171347713997382E-5	0.0	2.723782571332461E-5	5.1621944426664195	0.0
18	8.171347713997382E-5	0.0	2.723782571332461E-5	5.514951523479687	0.0
19	8.171347713997382E-5	0.0	2.723782571332461E-5	5.719235216329621	0.0
20	8.171347713997382E-5	0.0	2.723782571332461E-5	5.926569545659448	0.0
21	8.171347713997382E-5	0.0	2.723782571332461E-5	6.192655865052917	0.0
22	8.171347713997382E-5	0.0	2.723782571332461E-5	6.496003530022213	0.0
23	8.171347713997382E-5	0.0	2.723782571332461E-5	6.809592617459718	0.0
24	8.171347713997382E-5	0.0	2.723782571332461E-5	7.028094455332009	0.0
25	8.171347713997382E-5	0.0	2.723782571332461E-5	7.225704880882179	0.0
26	8.171347713997382E-5	0.0	2.723782571332461E-5	7.41767707650969	0.0
27	8.171347713997382E-5	0.0	2.723782571332461E-5	7.6396925938989995	0.0
28	8.171347713997382E-5	0.0	2.723782571332461E-5	7.858793663936982	0.0
29	8.171347713997382E-5	0.0	2.723782571332461E-5	8.114121042173688	0.0
30	8.171347713997382E-5	0.0	2.723782571332461E-5	8.420056300585749	0.0
31	8.171347713997382E-5	0.0	2.723782571332461E-5	8.684290447830712	0.0
32	8.171347713997382E-5	0.0	2.723782571332461E-5	8.924882162356507	0.0
33	8.171347713997382E-5	0.0	2.723782571332461E-5	9.174516835019128	0.0
34	8.171347713997382E-5	0.0	2.723782571332461E-5	9.436680907509878	0.0
35	8.171347713997382E-5	0.0	2.723782571332461E-5	9.735888422970747	0.0
36	8.171347713997382E-5	0.0	2.723782571332461E-5	9.998433825021484	0.0
37	8.171347713997382E-5	0.0	2.723782571332461E-5	10.273154535166077	0.0
38	8.171347713997382E-5	0.0	2.723782571332461E-5	10.585109353060783	0.0
39	8.171347713997382E-5	0.0	2.723782571332461E-5	11.047062877158767	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGA	30	2.1668093E-6	45.000004	23
TACGTCG	20	7.0353114E-4	45.0	2
CGTTTTT	14585	0.0	42.84025	1
CGTAAGG	620	0.0	41.73387	2
CGACGGT	465	0.0	41.612904	28
GCGTAAG	250	0.0	41.399998	1
ACGGGAT	2050	0.0	41.268295	5
CGTCGGT	60	3.6379788E-12	41.250004	12
TAGGGCG	785	0.0	40.12739	5
TATAGCG	320	0.0	40.078125	1
AATCGCG	90	0.0	40.000004	1
CGGTCTA	480	0.0	39.843754	31
TTCGCGG	235	0.0	39.255318	2
TACGGGA	1845	0.0	39.146343	4
GACCGAT	1520	0.0	38.782894	9
GGCGATA	1150	0.0	38.739132	8
GGGCGAT	4645	0.0	38.654465	7
CGGGATC	1505	0.0	38.571426	6
TCACGAC	550	0.0	38.454544	25
CTATGCG	170	0.0	38.38235	1
>>END_MODULE