FastQCFastQC Report
Thu 26 May 2016
SRR1547944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547944_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2803573
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98120.3499819694368579No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38440.1371107511735917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA351.2127566E-745.0000048
GTCGGTA207.035026E-445.020
GTACGTA253.8922473E-545.038
CGGTCTA7300.042.8424731
TTTCGCG1000.042.751
CGACGGT7300.042.5342528
CTCACGA8000.041.062524
ACGGGTA1050.040.7142875
TACGACG501.0822987E-940.51
GCGATAA1500.040.59
TCACGAC7950.040.471725
CACGACG7700.040.32467326
GACGGTC7700.040.32467329
TACGGGA14300.040.279724
CGTTTTT62750.039.7290841
CACGACC4100.039.51219627
CGTACTC403.460591E-739.37545
TTAGGGA44200.038.993214
ACGGGAT13400.038.9552235
CACAACG12650.038.77470412