##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547944_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2803573
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32827003256202	31.0	31.0	33.0	30.0	34.0
2	31.717223699900092	31.0	31.0	34.0	30.0	34.0
3	31.843796826406873	31.0	31.0	34.0	30.0	34.0
4	35.615615858763086	37.0	35.0	37.0	33.0	37.0
5	35.51912612940701	37.0	35.0	37.0	33.0	37.0
6	35.555373803357355	37.0	35.0	37.0	33.0	37.0
7	35.9039807417178	37.0	35.0	37.0	35.0	37.0
8	35.89893289741341	37.0	35.0	37.0	35.0	37.0
9	37.61018564524626	39.0	37.0	39.0	35.0	39.0
10	37.08766063876347	39.0	37.0	39.0	33.0	39.0
11	36.91177864817502	39.0	37.0	39.0	33.0	39.0
12	36.382665976594865	38.0	35.0	39.0	32.0	39.0
13	36.19060784220707	38.0	35.0	39.0	32.0	39.0
14	37.23150601036606	39.0	35.0	41.0	32.0	41.0
15	37.4074265232259	39.0	35.0	41.0	32.0	41.0
16	37.486594427896115	39.0	35.0	41.0	32.0	41.0
17	37.41127625355217	39.0	35.0	41.0	32.0	41.0
18	37.325328429115274	39.0	36.0	41.0	32.0	41.0
19	37.287254514150334	39.0	35.0	41.0	32.0	41.0
20	37.16082620284901	39.0	35.0	41.0	32.0	41.0
21	37.03777929092626	39.0	35.0	41.0	32.0	41.0
22	36.949997378345415	39.0	35.0	41.0	31.0	41.0
23	36.82602664528443	39.0	35.0	41.0	31.0	41.0
24	36.77968684960228	38.0	35.0	41.0	31.0	41.0
25	36.82185018902665	39.0	35.0	41.0	31.0	41.0
26	36.72180927694767	38.0	35.0	41.0	31.0	41.0
27	36.64410842877999	38.0	35.0	41.0	31.0	41.0
28	36.60010707764699	38.0	35.0	41.0	31.0	41.0
29	36.59370988378045	38.0	35.0	41.0	31.0	41.0
30	36.51897382375989	38.0	35.0	40.0	30.0	41.0
31	36.39082520769033	38.0	35.0	40.0	30.0	41.0
32	36.25316694090006	38.0	35.0	40.0	30.0	41.0
33	36.141790493773485	38.0	35.0	40.0	30.0	41.0
34	35.99912112151173	38.0	35.0	40.0	29.0	41.0
35	35.95312731289679	38.0	35.0	40.0	29.0	41.0
36	35.87000695184324	38.0	35.0	40.0	29.0	41.0
37	35.767347952059744	38.0	35.0	40.0	29.0	41.0
38	35.666591167770555	38.0	35.0	40.0	28.0	41.0
39	35.58840486764568	38.0	35.0	40.0	27.0	41.0
40	35.41842784190032	38.0	34.0	40.0	27.0	41.0
41	35.318048789883484	38.0	34.0	40.0	26.0	41.0
42	35.29331178464053	38.0	34.0	40.0	26.0	41.0
43	35.19584544436688	38.0	34.0	40.0	26.0	41.0
44	35.0518238690414	38.0	34.0	40.0	26.0	41.0
45	34.916941702605925	37.0	34.0	40.0	26.0	41.0
46	34.82106511940299	37.0	34.0	40.0	25.0	41.0
47	34.73914857933073	37.0	34.0	40.0	25.0	41.0
48	34.61351461153321	37.0	34.0	40.0	24.0	41.0
49	34.456707208979395	37.0	33.0	40.0	24.0	41.0
50	34.32896450351034	36.0	33.0	40.0	24.0	41.0
51	34.00008489167216	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	2.0
12	2.0
13	16.0
14	34.0
15	77.0
16	187.0
17	464.0
18	1110.0
19	2122.0
20	3667.0
21	5952.0
22	9350.0
23	13947.0
24	20619.0
25	29372.0
26	37900.0
27	44646.0
28	50573.0
29	59118.0
30	70077.0
31	84188.0
32	104544.0
33	133399.0
34	213840.0
35	296300.0
36	208116.0
37	272750.0
38	426752.0
39	714343.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.298028622761024	22.563992448208054	29.44346375143433	12.694515177596589
2	31.21284874693828	25.519827734109295	28.960508608122566	14.30681491082986
3	29.169028236468247	26.13946560335686	30.621638887234255	14.069867272940636
4	25.86074983601283	28.504091029554075	30.051544939261436	15.583614195171661
5	24.196088348689333	32.43860602167305	28.631000512560224	14.734305117077387
6	22.633617886889336	41.11788778105653	25.701952472790975	10.546541859263161
7	88.65993501863515	3.4672897762961763	6.02563229136534	1.847142913703335
8	88.25074289130335	3.0612365007082034	6.135848790097493	2.552171817890956
9	82.23984180187212	6.679226829477955	7.825549753831986	3.2553816148179484
10	34.9500797732037	41.02814515619889	13.230509781625091	10.791265288972323
11	34.65902261150325	24.442880567047833	25.448668538325915	15.449428283123002
12	32.78691155892856	22.876843228266218	26.980428189314136	17.355817023491095
13	24.76279376352961	31.280548072049484	27.32270570447069	16.633952459950212
14	18.631153888270433	34.31321388813489	28.92294939350607	18.132682830088605
15	17.259118988519294	27.894369078315425	38.03232517933366	16.814186753831628
16	21.190067103656656	26.194502515183306	35.98607919251612	16.62935118864392
17	20.852212515957316	25.430477465719637	28.18963515485418	25.527674863468867
18	22.005526519195325	25.79269382320346	32.086020232039616	20.115759425561595
19	24.941458631539113	27.000224356562143	28.068111656090284	19.990205355808463
20	27.16415802263754	27.085508385192753	26.764132769148514	18.986200823021196
21	23.539390627602703	27.901681176127745	30.10740223279365	18.45152596347589
22	23.513174081787778	24.296103579254044	28.60193046515999	23.588791873798183
23	20.430322306570936	29.486087931364725	28.470919073624977	21.61267068843936
24	19.793813109200293	26.654344295654152	33.75182311999723	19.80001947514832
25	19.768167263702423	28.025451807390066	31.207427093926217	20.99895383498129
26	20.268314754065617	31.174290806766937	28.030053078696366	20.527341360471084
27	19.97964739994286	30.893006887996137	29.063127658883857	20.06421805317714
28	17.170446426756143	29.56687769499849	32.768542142473194	20.494133735772174
29	19.44522222178627	27.14903446423546	32.32193347560416	21.083809838374105
30	21.28936895882504	28.08184413246953	30.127376743890743	20.501410164814686
31	24.49902321073858	28.039077277459867	26.82391362736051	20.637985884441033
32	24.778452353478936	28.28494210780315	27.670725891567656	19.26587964715026
33	24.06579033255064	27.47247886892904	27.3677910295184	21.093939769001913
34	19.52661835450691	28.59247824115869	29.933195961011183	21.947707443323218
35	19.875565929619096	27.953935923908528	30.259743548678774	21.910754597793602
36	24.854640845806404	27.657243096577115	27.524020241313497	19.964095816302983
37	20.21395554886568	31.15410228305095	28.645945727113226	19.985996440970148
38	20.263535138910242	30.572380316118043	26.997905886524087	22.16617865844763
39	20.122964517064474	29.11698750130637	28.041788103965903	22.718259877663254
40	22.57850963752326	25.98480581743368	29.215433305999166	22.22125123904389
41	19.048335820041068	26.320841297872395	29.400126196107614	25.230696685978927
42	22.183121324110342	27.426929849873716	27.221905760970017	23.168043065045925
43	21.753205641515308	27.12659880802105	28.049635233325475	23.070560317138167
44	20.443270070014226	28.793471759073153	28.216386732216353	22.546871438696265
45	19.920116223119567	30.341567706637207	26.47396732669347	23.26434874354975
46	21.334275940023677	28.92230735564938	28.09186705678789	21.65154964753905
47	21.20098174722042	28.244886079299526	28.391805742172576	22.162326431307477
48	20.687850824644123	27.395434326125983	29.75124956617859	22.165465283051304
49	21.64833945825559	25.554676122219753	30.112324523028295	22.684659896496363
50	19.870750645693906	29.12833017010793	29.014939150862133	21.985980033336034
51	19.532182682598243	28.41138789680169	27.564539963824735	24.491889456775336
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2135.0
1	3415.0
2	4695.0
3	11202.0
4	17709.0
5	13557.0
6	9405.0
7	9254.0
8	9103.0
9	9758.0
10	10413.0
11	10883.0
12	11353.0
13	11434.5
14	11516.0
15	11425.0
16	11334.0
17	11425.0
18	11516.0
19	11418.0
20	11320.0
21	11695.0
22	12070.0
23	14682.0
24	17294.0
25	20026.5
26	27390.0
27	32021.0
28	37796.5
29	43572.0
30	50708.5
31	57845.0
32	66719.0
33	75593.0
34	85972.5
35	96352.0
36	100875.0
37	105398.0
38	115989.0
39	126580.0
40	148694.0
41	170808.0
42	189964.0
43	209120.0
44	223573.5
45	238027.0
46	243555.0
47	249083.0
48	251884.5
49	254686.0
50	240991.5
51	227297.0
52	206498.0
53	185699.0
54	163020.0
55	140341.0
56	130857.5
57	121374.0
58	108990.0
59	96606.0
60	84030.0
61	71454.0
62	61782.5
63	52111.0
64	44052.5
65	35994.0
66	28692.0
67	21390.0
68	16855.0
69	12320.0
70	9932.5
71	7545.0
72	5798.0
73	4051.0
74	3320.0
75	2115.0
76	1641.0
77	1155.5
78	670.0
79	568.0
80	466.0
81	313.5
82	161.0
83	118.5
84	76.0
85	52.5
86	29.0
87	27.5
88	26.0
89	18.5
90	11.0
91	7.0
92	3.0
93	3.0
94	3.0
95	2.5
96	2.0
97	3.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2803573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.516172227113792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.78242787769149	15.54765342127548
2	9.897561750469917	4.061201630022694
3	3.614056086469543	2.22439791325374
4	1.769038431171385	1.4517558852118129
5	1.03469837564474	1.0614025038921187
6	0.6738835330706369	0.8295306375295871
7	0.4705431610951364	0.6757621173322857
8	0.35761834394699527	0.5869567628793418
9	0.2671041097141178	0.4931958525718264
>10	3.190069746078002	16.488133446070677
>50	2.0350933161604834	30.65337973889295
>100	0.9001258200518564	24.110693573724497
>500	0.005657780680598142	0.809412477487686
>1k	0.0019448621089556116	0.6506059960661081
>5k	1.7680564626869195E-4	0.35591804378906405
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9812	0.3499819694368579	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3844	0.1371107511735917	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1208457921373904	0.0
2	0.0	0.0	0.0	0.357258398479369	0.0
3	0.0	0.0	0.0	0.5387054305345358	0.0
4	0.0	0.0	0.0	0.8431740496858829	0.0
5	0.0	0.0	0.0	1.4176552563460985	0.0
6	0.0	0.0	0.0	1.9481925385927172	0.0
7	0.0	0.0	0.0	2.2449210346939426	0.0
8	0.0	0.0	0.0	3.0046658317796613	0.0
9	0.0	0.0	0.0	3.2455013655788525	0.0
10	0.0	0.0	0.0	3.7428666918963764	0.0
11	0.0	0.0	0.0	4.406020460319742	0.0
12	0.0	0.0	0.0	4.941052007563206	0.0
13	0.0	0.0	0.0	5.169831497164511	0.0
14	0.0	0.0	0.0	5.261036541584613	0.0
15	0.0	0.0	0.0	5.428465747102002	0.0
16	0.0	0.0	0.0	5.777270647134924	0.0
17	0.0	0.0	0.0	6.198447481124979	0.0
18	0.0	0.0	0.0	6.6864675897506505	0.0
19	0.0	0.0	0.0	6.947420309726196	0.0
20	0.0	0.0	0.0	7.240296578687268	0.0
21	0.0	0.0	0.0	7.603511661725948	0.0
22	3.566876981623093E-5	0.0	0.0	7.9980082558934615	0.0
23	3.566876981623093E-5	0.0	0.0	8.396927777518188	0.0
24	3.566876981623093E-5	0.0	0.0	8.696866462902875	0.0
25	3.566876981623093E-5	0.0	0.0	8.970196246004653	0.0
26	3.566876981623093E-5	0.0	0.0	9.237676350856567	0.0
27	3.566876981623093E-5	0.0	0.0	9.502766648130796	0.0
28	3.566876981623093E-5	0.0	0.0	9.786618718328361	0.0
29	3.566876981623093E-5	0.0	0.0	10.089446574068162	0.0
30	3.566876981623093E-5	0.0	0.0	10.453268026193717	0.0
31	3.566876981623093E-5	0.0	0.0	10.790658919885447	0.0
32	3.566876981623093E-5	0.0	0.0	11.098302059550438	0.0
33	3.566876981623093E-5	0.0	0.0	11.416681498930116	0.0
34	3.566876981623093E-5	0.0	0.0	11.741838004574877	0.0
35	3.566876981623093E-5	0.0	0.0	12.107835251659221	0.0
36	3.566876981623093E-5	0.0	0.0	12.438805766784029	0.0
37	3.566876981623093E-5	0.0	0.0	12.77512659738127	0.0
38	3.566876981623093E-5	0.0	0.0	13.107702207147808	0.0
39	3.566876981623093E-5	0.0	0.0	13.517750384955198	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	35	1.2127566E-7	45.000004	8
GTCGGTA	20	7.035026E-4	45.0	20
GTACGTA	25	3.8922473E-5	45.0	38
CGGTCTA	730	0.0	42.84247	31
TTTCGCG	100	0.0	42.75	1
CGACGGT	730	0.0	42.53425	28
CTCACGA	800	0.0	41.0625	24
ACGGGTA	105	0.0	40.714287	5
TACGACG	50	1.0822987E-9	40.5	1
GCGATAA	150	0.0	40.5	9
TCACGAC	795	0.0	40.4717	25
CACGACG	770	0.0	40.324673	26
GACGGTC	770	0.0	40.324673	29
TACGGGA	1430	0.0	40.27972	4
CGTTTTT	6275	0.0	39.729084	1
CACGACC	410	0.0	39.512196	27
CGTACTC	40	3.460591E-7	39.375	45
TTAGGGA	4420	0.0	38.99321	4
ACGGGAT	1340	0.0	38.955223	5
CACAACG	1265	0.0	38.774704	12
>>END_MODULE