FastQCFastQC Report
Thu 26 May 2016
SRR1547943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547943_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3823893
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220750.5772912578882307No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48990.128115509508242No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA207.035348E-445.029
TAATACG302.166833E-644.9999961
CGACGGT9050.043.0110528
CGTTTTT106950.041.928471
CGGTCTA9550.040.5235631
ATAACGC2600.039.80769311
CGCGTCA1650.039.54545239
TCGTAAG2450.039.4897961
CGCATCG2250.039.021
TAGTACG1850.038.918921
GCGCGAC6050.038.6776859
TAACGCC2700.038.33333612
GACCGAT15800.038.306969
ATAGCGG6350.038.2677152
TAAGGGA47000.038.202134
TAGGGCG13200.038.1818165
CTCACGA10550.038.17535824
ACGGGAT18650.038.1233255
TCACGAC10600.037.99528525
TCGATAG1600.037.968751
Produced by FastQC (version 0.11.5)