##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547943_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3823893
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303143681060114	31.0	31.0	33.0	30.0	34.0
2	31.69487299984597	31.0	31.0	34.0	30.0	34.0
3	31.802202624393516	31.0	31.0	34.0	30.0	34.0
4	35.600463977417775	37.0	35.0	37.0	33.0	37.0
5	35.50191310269403	37.0	35.0	37.0	33.0	37.0
6	35.552423145731325	37.0	35.0	37.0	33.0	37.0
7	35.89047653791568	37.0	35.0	37.0	35.0	37.0
8	35.89977857644029	37.0	35.0	37.0	35.0	37.0
9	37.67797765261737	39.0	37.0	39.0	35.0	39.0
10	37.079699405814964	39.0	37.0	39.0	33.0	39.0
11	36.846594033881175	39.0	37.0	39.0	32.0	39.0
12	36.31332179012331	38.0	35.0	39.0	32.0	39.0
13	36.10123321965337	38.0	35.0	39.0	32.0	39.0
14	36.96802081020572	39.0	35.0	41.0	31.0	41.0
15	37.19560223050174	39.0	35.0	41.0	31.0	41.0
16	37.308158465731125	39.0	35.0	41.0	32.0	41.0
17	37.263064369217446	39.0	35.0	41.0	32.0	41.0
18	37.18387256128767	39.0	35.0	41.0	32.0	41.0
19	37.1358570964198	39.0	35.0	41.0	32.0	41.0
20	36.97419671523236	39.0	35.0	41.0	31.0	41.0
21	36.88088238870701	39.0	35.0	41.0	31.0	41.0
22	36.8403953771719	39.0	35.0	41.0	31.0	41.0
23	36.713967153369616	38.0	35.0	41.0	31.0	41.0
24	36.66789211936631	38.0	35.0	40.0	31.0	41.0
25	36.67729065640697	38.0	35.0	41.0	31.0	41.0
26	36.589120563781464	38.0	35.0	41.0	31.0	41.0
27	36.52643287874425	38.0	35.0	40.0	31.0	41.0
28	36.47374782714893	38.0	35.0	40.0	31.0	41.0
29	36.43869585262977	38.0	35.0	40.0	30.0	41.0
30	36.33930107353945	38.0	35.0	40.0	30.0	41.0
31	36.187107222927004	38.0	35.0	40.0	30.0	41.0
32	36.08770721356481	38.0	35.0	40.0	30.0	41.0
33	35.967484445825235	38.0	35.0	40.0	30.0	41.0
34	35.78916329510266	38.0	35.0	40.0	29.0	41.0
35	35.74365731467905	38.0	34.0	40.0	29.0	41.0
36	35.66021094209488	38.0	34.0	40.0	28.0	41.0
37	35.565509547469034	38.0	34.0	40.0	27.0	41.0
38	35.428438243434115	38.0	34.0	40.0	27.0	41.0
39	35.364862719746604	38.0	34.0	40.0	27.0	41.0
40	35.19981207633163	38.0	34.0	40.0	26.0	41.0
41	35.08227845287512	38.0	34.0	40.0	25.0	41.0
42	35.089057669762205	38.0	34.0	40.0	26.0	41.0
43	35.03811168356437	38.0	34.0	40.0	25.0	41.0
44	34.91410925985638	38.0	34.0	40.0	25.0	41.0
45	34.81643340961685	37.0	34.0	40.0	25.0	41.0
46	34.7669566067879	37.0	34.0	40.0	24.0	41.0
47	34.68411119244184	37.0	34.0	40.0	24.0	41.0
48	34.53987676956442	37.0	33.0	40.0	24.0	41.0
49	34.396027556210385	37.0	33.0	40.0	24.0	41.0
50	34.30750415871992	36.0	33.0	40.0	24.0	41.0
51	33.99679750453269	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	7.0
13	17.0
14	43.0
15	116.0
16	309.0
17	779.0
18	1780.0
19	3396.0
20	5715.0
21	9416.0
22	14152.0
23	20759.0
24	30148.0
25	43148.0
26	56361.0
27	66160.0
28	72951.0
29	82154.0
30	97641.0
31	118719.0
32	147106.0
33	188084.0
34	301660.0
35	387204.0
36	285657.0
37	369425.0
38	571704.0
39	949133.0
40	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.298602497507126	24.63973233560667	30.150085266507197	12.91157990037901
2	30.69497499014748	25.752420373687237	30.26936161655151	13.28324301961378
3	28.700750779375888	25.60150087881643	31.88598634951344	13.811761992294242
4	26.214854861263113	27.43311593708297	31.369444699420196	14.982584502233717
5	23.188018074773538	32.3895307740044	30.289602768696717	14.132848382525347
6	21.514958708311138	41.276442620125614	26.707284958025763	10.501313713537487
7	89.58446274516572	3.01757920527588	5.86480845567593	1.5331495938824649
8	90.44818984213208	2.2914082585469835	5.520604263769933	1.7397976355509948
9	85.74447559071345	4.911068379789916	7.00118962533732	2.3432664041593214
10	39.72522243692488	35.086415859439576	13.460235419767239	11.728126283868297
11	35.38679037305699	22.920803484825544	25.050674796601264	16.641731345516206
12	34.081236059691	21.985290906413958	26.276650523432533	17.656822510462504
13	27.04089784939066	26.515543191192847	28.182404685486755	18.261154273929737
14	19.748565140290275	30.940954676294552	29.908315949217197	19.402164234197976
15	19.033273159055444	26.58711946176318	37.00838386429746	17.371223514883916
16	24.389777642836762	24.260066900407516	33.757717593039345	17.592437863716377
17	24.043899763931677	23.792768259990538	27.546011355443262	24.61732062063452
18	24.20541055934358	26.25473568428824	30.646987245720524	18.892866510647657
19	26.228244357255814	27.056301000054134	26.85985198853629	19.855602654153763
20	26.166108727414706	28.873114388922495	26.007474581532485	18.953302302130314
21	24.20038949834632	26.96568653986919	30.721466317179903	18.112457644604596
22	23.33577325516169	23.50583554508455	30.58453779956709	22.573853400186668
23	21.604160995090606	27.09592030948565	29.943044954448254	21.356873740975495
24	22.17271769895235	26.818454386668243	31.977751469510263	19.031076444869143
25	22.4227769971597	26.3195649041435	29.97382510441584	21.28383299428096
26	20.14823636540039	29.337222563497463	27.15575461970301	23.358786451399137
27	19.583759273598922	29.281180200387407	30.682788456685373	20.452272069328302
28	18.307049909607827	28.92588783211246	31.60067501888782	21.1663872393919
29	20.330223675191746	27.595620484150572	30.53260119987667	21.541554640781005
30	22.12789426900805	27.357852324842774	30.42658358902825	20.08766981712093
31	24.46757793693495	26.674491153387397	28.321399160489065	20.536531749188587
32	26.37003702770972	26.44485606684078	27.50898103058846	19.67612587486104
33	25.484604302473947	26.69332013212713	27.098090872312586	20.72398469308634
34	20.147634884135098	27.981536094236947	30.18104847599031	21.689780545637653
35	20.4281605159977	28.011348643908185	29.062476382053575	22.498014458040537
36	24.91141880800535	27.530843567014035	27.87039281695382	19.687344808026793
37	21.459988550934874	30.476872653078946	28.093490063660255	19.969648732325933
38	21.84470119849065	29.788098150235896	26.803521960473265	21.563678690800188
39	20.895380702336595	27.97871174742599	28.02934077914837	23.096566771089044
40	22.702230423288515	25.904621285166712	28.8251527958549	22.567995495689864
41	20.027940112341007	25.8322343224562	28.677789885857162	25.46203567934563
42	21.32086331913576	27.295324424611255	27.962942477731463	23.42086977852152
43	21.238277326274556	26.590153019449026	28.480164063168083	23.691405591108328
44	21.472802717021633	27.94963143581685	27.614815581921352	22.96275026524016
45	20.646838182972168	29.383641226362766	26.153111501812422	23.816409088852645
46	21.84132767313311	28.468945130002332	27.467818790954663	22.221908405909893
47	20.80790440527494	28.101518530984	28.63492780786492	22.455649255876146
48	20.986936611458532	27.772926700616363	29.414918252158206	21.8252184357669
49	22.46548216699578	25.16995114664558	29.403097837727156	22.961468848631487
50	20.51035423846849	27.88642360024195	28.762363382029783	22.840858779259776
51	19.470314676692052	28.449148550966253	28.04283488057851	24.037701891763184
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2195.0
1	3950.5
2	5706.0
3	20380.0
4	35054.0
5	25094.5
6	15135.0
7	14352.0
8	13569.0
9	14465.0
10	15361.0
11	16391.0
12	17421.0
13	17327.5
14	17234.0
15	16524.5
16	15815.0
17	14662.5
18	13510.0
19	13626.0
20	13742.0
21	14209.0
22	14676.0
23	16736.0
24	18796.0
25	22596.0
26	32487.5
27	38579.0
28	44148.0
29	49717.0
30	59385.0
31	69053.0
32	79252.5
33	89452.0
34	97187.5
35	104923.0
36	118874.5
37	132826.0
38	143988.0
39	155150.0
40	179359.0
41	203568.0
42	230726.5
43	257885.0
44	276226.0
45	294567.0
46	305548.5
47	316530.0
48	328220.5
49	339911.0
50	321848.5
51	303786.0
52	285169.5
53	266553.0
54	249556.0
55	232559.0
56	219496.0
57	206433.0
58	191799.5
59	177166.0
60	155751.0
61	134336.0
62	114200.5
63	94065.0
64	77557.0
65	61049.0
66	47052.0
67	33055.0
68	25149.5
69	17244.0
70	13264.5
71	9285.0
72	7523.0
73	5761.0
74	4403.5
75	2245.0
76	1444.0
77	1084.0
78	724.0
79	508.0
80	292.0
81	227.5
82	163.0
83	120.5
84	78.0
85	63.5
86	49.0
87	31.0
88	13.0
89	11.0
90	9.0
91	6.0
92	3.0
93	3.5
94	4.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3823893.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.590430420708024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.02700500348905	15.654287566191622
2	9.595279267878528	3.9514186026503015
3	3.4787220333682916	2.1488515194316125
4	1.727349562701401	1.4226748393217452
5	1.0250401094439798	1.0553008525970604
6	0.6825897025258926	0.8432889465450708
7	0.49272277200806064	0.7101761767611249
8	0.3881070613928123	0.6393033154715327
9	0.3097974797994044	0.5740977107088303
>10	3.496974975671304	17.715207602528686
>50	1.8184049194193954	27.3407565983093
>100	0.9506234290729354	25.892294546504807
>500	0.004922455485971408	0.6472880842829648
>1k	0.002331689440723292	0.8162583337445
>5k	0.0	0.0
>10k+	1.2953830226240513E-4	0.5887953049508164
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22075	0.5772912578882307	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4899	0.128115509508242	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.2302718721470503E-5	0.0	0.0	0.0591282235146224	0.0
2	5.2302718721470503E-5	0.0	0.0	0.19469687044067394	0.0
3	5.2302718721470503E-5	0.0	0.0	0.29974688099274743	0.0
4	5.2302718721470503E-5	0.0	0.0	0.4728165772420933	0.0
5	5.2302718721470503E-5	0.0	0.0	0.8634394320133958	0.0
6	5.2302718721470503E-5	0.0	0.0	1.2724728437746557	0.0
7	5.2302718721470503E-5	0.0	0.0	1.534431010491141	0.0
8	5.2302718721470503E-5	0.0	0.0	2.13549385403828	0.0
9	5.2302718721470503E-5	0.0	0.0	2.368188649630102	0.0
10	5.2302718721470503E-5	0.0	0.0	2.7970447917867993	0.0
11	5.2302718721470503E-5	0.0	0.0	3.329721830605616	0.0
12	5.2302718721470503E-5	0.0	0.0	3.7403243239285198	0.0
13	5.2302718721470503E-5	0.0	0.0	3.9150415558175924	0.0
14	5.2302718721470503E-5	0.0	0.0	3.9985951489751415	0.0
15	5.2302718721470503E-5	0.0	0.0	4.11128135646055	0.0
16	5.2302718721470503E-5	0.0	0.0	4.35493357162452	0.0
17	5.2302718721470503E-5	0.0	0.0	4.6465212284967174	0.0
18	5.2302718721470503E-5	0.0	0.0	5.014941579170756	0.0
19	5.2302718721470503E-5	0.0	0.0	5.22459702716577	0.0
20	5.2302718721470503E-5	0.0	0.0	5.468903026313759	0.0
21	5.2302718721470503E-5	0.0	0.0	5.779241207847605	0.0
22	5.2302718721470503E-5	0.0	0.0	6.123314642956799	0.0
23	5.2302718721470503E-5	0.0	0.0	6.469663246330376	0.0
24	5.2302718721470503E-5	0.0	0.0	6.737113198512615	0.0
25	5.2302718721470503E-5	0.0	0.0	6.9726846436341186	0.0
26	5.2302718721470503E-5	0.0	0.0	7.205222531069777	0.0
27	5.2302718721470503E-5	0.0	0.0	7.473692386267085	0.0
28	5.2302718721470503E-5	0.0	0.0	7.738474899794529	0.0
29	5.2302718721470503E-5	0.0	0.0	8.052683482513762	0.0
30	5.2302718721470503E-5	0.0	0.0	8.393200332749897	0.0
31	5.2302718721470503E-5	0.0	0.0	8.709317964702464	0.0
32	5.2302718721470503E-5	0.0	0.0	9.009143299773294	0.0
33	5.2302718721470503E-5	0.0	0.0	9.309622418828141	0.0
34	5.2302718721470503E-5	0.0	0.0	9.638789579101717	0.0
35	5.2302718721470503E-5	0.0	0.0	10.00208426334105	0.0
36	5.2302718721470503E-5	0.0	0.0	10.322438415510057	0.0
37	5.2302718721470503E-5	0.0	0.0	10.652259359767651	0.0
38	5.2302718721470503E-5	0.0	0.0	11.057788489374571	0.0
39	5.2302718721470503E-5	0.0	0.0	11.73707004876967	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCGA	20	7.035348E-4	45.0	29
TAATACG	30	2.166833E-6	44.999996	1
CGACGGT	905	0.0	43.01105	28
CGTTTTT	10695	0.0	41.92847	1
CGGTCTA	955	0.0	40.52356	31
ATAACGC	260	0.0	39.807693	11
CGCGTCA	165	0.0	39.545452	39
TCGTAAG	245	0.0	39.489796	1
CGCATCG	225	0.0	39.0	21
TAGTACG	185	0.0	38.91892	1
GCGCGAC	605	0.0	38.677685	9
TAACGCC	270	0.0	38.333336	12
GACCGAT	1580	0.0	38.30696	9
ATAGCGG	635	0.0	38.267715	2
TAAGGGA	4700	0.0	38.20213	4
TAGGGCG	1320	0.0	38.181816	5
CTCACGA	1055	0.0	38.175358	24
ACGGGAT	1865	0.0	38.123325	5
TCACGAC	1060	0.0	37.995285	25
TCGATAG	160	0.0	37.96875	1
>>END_MODULE