FastQCFastQC Report
Thu 26 May 2016
SRR1547942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547942_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3232631
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194460.6015533477220258No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT34090.1054558964509095No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG207.0351857E-445.00000438
CGACGGT7000.042.10714328
TCACGAC7400.041.65540325
CGTTTTT93400.041.458781
TAGTACG1100.040.9090921
CGGTCTA6900.040.7608731
CGTAAGG4350.040.344832
CTCACGA7800.040.09615324
CACGACG7250.040.0344826
TTAGGGA56850.038.7467044
GGGCGAT42450.038.268557
TAAGGGA52050.038.0403444
TATGGGA46650.037.8617364
TAGGGAC42650.037.7725685
TCTCACG8250.037.63636423
GACGGTC7850.037.5477729
TCGATTG1200.037.5000041
ACGGACG301.1403795E-437.5000041
TACGGGA15600.037.54
ATGGGAC29350.037.33395
Produced by FastQC (version 0.11.5)