FastQCFastQC Report
Thu 26 May 2016
SRR1547941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547941_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2353829
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326121.3854872210343232No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27460.11666098089538365No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG302.1664764E-645.0000041
CGACGTA207.0347945E-445.016
CGTTTTT145350.043.2817341
TAACGCG750.042.0000041
CGACGGT2700.041.66666828
ACGTGCG556.184564E-1140.9090921
CGGTCTA2700.040.83333631
TTTCGCG1000.040.51
CGAACGG1300.039.807692
ACGGGAT10000.039.1499985
ATTGCGG4550.038.5714262
TACGGGA8050.038.2919244
CGGGATA4200.038.0357136
GCACCGA8100.037.7777759
AGGGCGA18550.037.722376
GACCGAT9150.037.622959
GGGCGAT36500.037.6027377
CGTTCCG301.1402854E-437.50000416
GGCGATT9700.037.3453648
GCGGGAT13200.037.1590925
Produced by FastQC (version 0.11.5)