##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547941_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2353829
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.339089628006114	31.0	31.0	33.0	30.0	34.0
2	31.721360387691714	31.0	31.0	34.0	30.0	34.0
3	31.68045682162978	31.0	31.0	34.0	30.0	34.0
4	35.55966172563937	37.0	35.0	37.0	33.0	37.0
5	35.53693152731146	37.0	35.0	37.0	33.0	37.0
6	35.601926478091656	37.0	35.0	37.0	33.0	37.0
7	35.90841008416499	37.0	35.0	37.0	35.0	37.0
8	35.92457523464959	37.0	35.0	37.0	35.0	37.0
9	37.71225649781696	39.0	37.0	39.0	35.0	39.0
10	37.10845010406448	39.0	37.0	39.0	33.0	39.0
11	36.9804875375399	39.0	37.0	39.0	33.0	39.0
12	36.57995759250141	39.0	35.0	39.0	32.0	39.0
13	36.42027054641607	39.0	35.0	39.0	32.0	39.0
14	37.42671493978535	40.0	36.0	41.0	32.0	41.0
15	37.58941707320285	40.0	36.0	41.0	32.0	41.0
16	37.670759005858116	40.0	36.0	41.0	33.0	41.0
17	37.607607434524766	40.0	36.0	41.0	32.0	41.0
18	37.480030197605686	39.0	36.0	41.0	32.0	41.0
19	37.36585410410017	39.0	36.0	41.0	32.0	41.0
20	37.149186708125356	39.0	35.0	41.0	32.0	41.0
21	37.05223574014935	39.0	35.0	41.0	32.0	41.0
22	36.99862224486146	39.0	35.0	41.0	32.0	41.0
23	36.88077978476771	39.0	35.0	41.0	31.0	41.0
24	36.830114676979505	38.0	35.0	41.0	31.0	41.0
25	36.84238234808051	38.0	35.0	41.0	31.0	41.0
26	36.753017317740586	38.0	35.0	41.0	31.0	41.0
27	36.681901276600804	38.0	35.0	41.0	31.0	41.0
28	36.6216726023853	38.0	35.0	41.0	31.0	41.0
29	36.6050426772718	38.0	35.0	41.0	31.0	41.0
30	36.513777338965575	38.0	35.0	41.0	31.0	41.0
31	36.34451907933839	38.0	35.0	41.0	30.0	41.0
32	36.14259829409868	38.0	35.0	41.0	30.0	41.0
33	35.9185828707183	38.0	35.0	41.0	29.0	41.0
34	35.67868906364906	38.0	35.0	41.0	27.0	41.0
35	35.531315996191736	38.0	35.0	41.0	27.0	41.0
36	35.41347481061708	38.0	35.0	41.0	25.0	41.0
37	35.30868300118658	38.0	34.0	40.0	25.0	41.0
38	35.17493369314423	38.0	34.0	40.0	24.0	41.0
39	35.10128093417151	38.0	34.0	40.0	24.0	41.0
40	34.95709204024591	38.0	34.0	40.0	23.0	41.0
41	34.83978955140751	38.0	34.0	40.0	23.0	41.0
42	34.8344144795565	38.0	34.0	40.0	23.0	41.0
43	34.79221302821913	38.0	34.0	40.0	23.0	41.0
44	34.6704722390624	38.0	34.0	40.0	23.0	41.0
45	34.58462658077541	38.0	34.0	40.0	23.0	41.0
46	34.54864775648529	38.0	34.0	40.0	23.0	41.0
47	34.49114103021078	38.0	34.0	40.0	23.0	41.0
48	34.357828032537626	37.0	33.0	40.0	23.0	41.0
49	34.23154060893973	37.0	33.0	40.0	23.0	41.0
50	34.1628168401358	37.0	33.0	40.0	22.0	41.0
51	33.885960704877036	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	8.0
13	10.0
14	18.0
15	67.0
16	208.0
17	489.0
18	1037.0
19	2066.0
20	3535.0
21	5760.0
22	8660.0
23	13300.0
24	20254.0
25	30982.0
26	42795.0
27	47905.0
28	48873.0
29	51515.0
30	57019.0
31	67656.0
32	82020.0
33	105476.0
34	165205.0
35	222337.0
36	170007.0
37	222485.0
38	357434.0
39	626617.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.78726534510366	24.868713912522956	29.87018173367734	16.473839008696046
2	33.739961569000975	24.597368797818365	28.61524775164211	13.047421881538549
3	27.88473589202954	24.67392491128285	34.12643824169045	13.314900954997155
4	24.39378561484288	26.70763254255088	34.25648167305271	14.642100169553524
5	22.69973732161512	31.138625618088657	32.40184397422243	13.759793086073795
6	21.721968758138335	38.86667213293744	29.15258500086455	10.258774108059676
7	86.7026874084736	2.53939432303706	9.422859519531793	1.3350587489575496
8	87.37512368145688	1.9074027892425491	9.12708612222893	1.5903874070716268
9	81.9854798288236	4.288119485315203	10.900664406802703	2.8257362790585043
10	32.534521411708326	30.44218590220445	21.03177418580534	15.991518500281881
11	31.015422105853908	19.83500075833886	29.64760821622981	19.50196891957742
12	31.35130037058767	20.769478156654543	29.638261742887867	18.240959729869928
13	25.269762586831924	25.29771703891829	31.611429717281926	17.821090656967858
14	19.26813715014982	28.69668952162625	33.0769567372991	18.958216590924827
15	18.198518244103543	26.16749135132586	38.72554038547405	16.908450019096545
16	22.454817236086395	24.157489775170585	36.26363682323567	17.12405616550735
17	21.936002997668904	23.513900117638112	32.15441733447927	22.395679550213714
18	22.220305723143017	24.348370251194968	34.360057591269374	19.07126643439264
19	23.70354855854015	25.572290935322826	31.172018018301245	19.552142487835777
20	23.93287702717572	26.865672909969245	30.933258108384255	18.26819195447078
21	22.841803716412702	25.63121620134683	33.43675347699429	18.090226605246176
22	21.74665194455502	22.697273251370426	34.080215682617556	21.475859121457
23	19.051766292283766	26.086771808827237	33.845321813946555	21.01614008494245
24	18.948190374067106	26.53799405139456	35.5767560005421	18.937059573996244
25	19.993083609726963	26.753812617654045	33.56785900759996	19.68524476501904
26	19.254074956167162	29.04934895440578	30.95959816962065	20.73697791980641
27	18.66397261653247	28.346451675121685	33.970734492607576	19.018841215738274
28	17.141856948826785	27.321525905237802	35.59306984492076	19.943547301014643
29	18.6248448804055	25.88896644573586	35.04753318953926	20.43865548431938
30	20.157326636726797	26.244472304487708	34.16284700375431	19.435354055031183
31	21.93485593048603	26.226416617349855	32.109936618165555	19.72879083399856
32	22.76486524722059	26.36368232356726	31.36200633096117	19.509446098250976
33	21.742658451399826	26.903568610973867	31.562870539873543	19.79090239775277
34	18.584315173277243	27.76913701037756	32.86819051001581	20.77835730632939
35	18.397428190408053	27.801934635013843	32.395428894792275	21.405208279785832
36	22.366747966823418	27.833330288648835	30.20155669761907	19.598365046908675
37	19.353784833137837	29.56569912257857	31.0853932040093	19.99512284027429
38	19.524697843386242	29.728370242698176	29.943466581472144	20.803465332443434
39	19.54313588625172	28.76542858465929	30.096281420612968	21.59515410847602
40	21.08594124721889	27.01878513689822	30.546611499815835	21.348662116067054
41	18.75688505834536	27.06857634942895	30.49614054376932	23.678398048456366
42	20.012158912138478	27.592531148184513	30.096238936643232	22.29907100303378
43	19.97787434856143	27.943958545841692	30.497839902558766	21.580327203038113
44	20.270333996224874	28.49935148220198	30.2268771435818	21.00343737799135
45	18.95056947637233	29.543225102588167	28.962809108053307	22.543396312986204
46	20.02932243591187	28.47645262251421	29.967597476282258	21.52662746529166
47	19.326297704718566	28.072005230626353	31.27737826324682	21.32431880140826
48	19.963812154578772	27.977138526205596	31.1698513358447	20.889197983370924
49	20.36715496325349	26.558598776716575	31.15273029604105	21.921515963988888
50	18.5180401804889	28.603182304237052	31.200439794054706	21.67833772121934
51	18.110703878658985	29.226082268508037	30.17963496923523	22.483578883597747
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1393.0
1	2611.0
2	3829.0
3	26952.5
4	50076.0
5	35351.0
6	20626.0
7	18558.0
8	16490.0
9	16645.5
10	16801.0
11	17026.0
12	17251.0
13	16563.0
14	15875.0
15	15107.0
16	14339.0
17	13655.5
18	12972.0
19	12915.5
20	12859.0
21	12785.0
22	12711.0
23	13539.0
24	14367.0
25	16304.5
26	22149.5
27	26057.0
28	30284.5
29	34512.0
30	41603.5
31	48695.0
32	56383.5
33	64072.0
34	68853.5
35	73635.0
36	81262.5
37	88890.0
38	98982.5
39	109075.0
40	123985.5
41	138896.0
42	155803.0
43	172710.0
44	181817.0
45	190924.0
46	194316.5
47	197709.0
48	192379.0
49	187049.0
50	176262.5
51	165476.0
52	154332.5
53	143189.0
54	132966.5
55	122744.0
56	116439.0
57	110134.0
58	97685.0
59	85236.0
60	73538.0
61	61840.0
62	52474.5
63	43109.0
64	35172.5
65	27236.0
66	21693.5
67	16151.0
68	12418.0
69	8685.0
70	6644.0
71	4603.0
72	3375.5
73	2148.0
74	1789.0
75	1236.5
76	1043.0
77	737.5
78	432.0
79	300.5
80	169.0
81	122.5
82	76.0
83	57.5
84	39.0
85	24.0
86	9.0
87	10.5
88	12.0
89	10.5
90	9.0
91	6.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2353829.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.320870093303576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.73456884996251	18.41930610572294
2	7.544689588181219	3.66986830736911
3	2.865488503538512	2.09073520945245
4	1.6565872287920467	1.6115857115870855
5	1.0995175593843267	1.3370611863546207
6	0.8252045286280741	1.2041815284701523
7	0.6628910153115356	1.1285460388586979
8	0.5477807745378294	1.0658004045715013
9	0.47905623231900935	1.0485957954256278
>10	7.213062375533149	43.46873110933435
>50	1.2992967459532172	20.224747873803036
>100	0.06829945483217413	2.522341627308762
>500	0.002667857269838267	0.4618053981940504
>1k	7.114286052902045E-4	0.3360157674383087
>5k	0.0	0.0
>10k+	1.7785715132255113E-4	1.4106779361094393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32612	1.3854872210343232	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2746	0.11666098089538365	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.043885940737411255	0.0
2	0.0	0.0	0.0	0.13730819018713764	0.0
3	0.0	0.0	0.0	0.23587099997493446	0.0
4	0.0	0.0	0.0	0.36774124203584885	0.0
5	0.0	0.0	0.0	0.6915965433342864	0.0
6	0.0	0.0	0.0	1.1396749721411368	0.0
7	0.0	0.0	0.0	1.4387196351136808	0.0
8	0.0	0.0	0.0	1.9798804416123685	0.0
9	0.0	0.0	0.0	2.255898792987936	0.0
10	0.0	0.0	0.0	2.6569474672969022	0.0
11	0.0	0.0	0.0	3.0774537997450113	0.0
12	0.0	0.0	0.0	3.380024632205653	0.0
13	0.0	0.0	0.0	3.5225583506703333	0.0
14	0.0	0.0	0.0	3.61598060012006	0.0
15	0.0	0.0	0.0	3.7030302541093683	0.0
16	0.0	0.0	0.0	3.874283136115665	0.0
17	0.0	0.0	0.0	4.090866413830401	0.0
18	0.0	0.0	0.0	4.345515328428701	0.0
19	0.0	0.0	0.0	4.499307298873452	0.0
20	0.0	0.0	0.0	4.675615773278348	0.0
21	0.0	0.0	0.0	4.902140299911336	0.0
22	0.0	0.0	0.0	5.15870099314776	0.0
23	0.0	0.0	0.0	5.418830339842019	0.0
24	0.0	0.0	0.0	5.616040927357085	0.0
25	0.0	0.0	0.0	5.802375618619704	0.0
26	0.0	0.0	0.0	5.982422682361378	0.0
27	0.0	0.0	0.0	6.181927404242194	0.0
28	0.0	0.0	0.0	6.387719753643957	0.0
29	0.0	0.0	0.0	6.622995978042585	0.0
30	0.0	0.0	0.0	6.910739905065321	0.0
31	0.0	0.0	0.0	7.156722089837452	0.0
32	0.0	0.0	0.0	7.382694324863871	0.0
33	0.0	0.0	0.0	7.609771143103428	0.0
34	0.0	0.0	0.0	7.859491917212337	0.0
35	0.0	0.0	0.0	8.141033184653601	0.0
36	0.0	0.0	0.0	8.38242710069423	0.0
37	0.0	0.0	0.0	8.636226335897808	0.0
38	0.0	0.0	0.0	8.930385342350698	0.0
39	0.0	0.0	0.0	9.396264554476982	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGCG	30	2.1664764E-6	45.000004	1
CGACGTA	20	7.0347945E-4	45.0	16
CGTTTTT	14535	0.0	43.281734	1
TAACGCG	75	0.0	42.000004	1
CGACGGT	270	0.0	41.666668	28
ACGTGCG	55	6.184564E-11	40.909092	1
CGGTCTA	270	0.0	40.833336	31
TTTCGCG	100	0.0	40.5	1
CGAACGG	130	0.0	39.80769	2
ACGGGAT	1000	0.0	39.149998	5
ATTGCGG	455	0.0	38.571426	2
TACGGGA	805	0.0	38.291924	4
CGGGATA	420	0.0	38.035713	6
GCACCGA	810	0.0	37.777775	9
AGGGCGA	1855	0.0	37.72237	6
GACCGAT	915	0.0	37.62295	9
GGGCGAT	3650	0.0	37.602737	7
CGTTCCG	30	1.1402854E-4	37.500004	16
GGCGATT	970	0.0	37.345364	8
GCGGGAT	1320	0.0	37.159092	5
>>END_MODULE