FastQCFastQC Report
Thu 26 May 2016
SRR1547940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547940_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4135834
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438371.0599313221952333No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA207.0354174E-445.012
CGTTTTT231800.043.281921
CGACGGT6150.040.243928
ACGGGAT25300.038.9525685
GGCGATA14450.038.7716268
TACGGGA21900.038.7328764
GCGTAAG2450.038.571431
GGGCGAT62200.038.2717067
ATACGCG1000.038.2500041
CGGACGG2550.037.9411772
TAAGGGA62500.037.9079974
CGTAAGG6300.037.8571432
TCGATAG2200.037.8409081
CGGGATT22100.037.7714926
AGGGATT91400.037.614886
TTGCGAG3600.037.51
CGGTCTA6600.037.531
CTCACGA7400.037.3986524
GACGGTC6800.037.3897129
TCACGAC7300.037.29451825
Produced by FastQC (version 0.11.5)