##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547940_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4135834
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.373119182249578	31.0	31.0	33.0	30.0	34.0
2	31.756909972692327	31.0	31.0	34.0	30.0	34.0
3	31.742371913379504	31.0	31.0	34.0	30.0	34.0
4	35.593247456256705	37.0	35.0	37.0	33.0	37.0
5	35.56050146113214	37.0	35.0	37.0	33.0	37.0
6	35.63198716389488	37.0	35.0	37.0	33.0	37.0
7	35.95463526824336	37.0	35.0	37.0	35.0	37.0
8	35.973724525694216	37.0	35.0	37.0	35.0	37.0
9	37.76322792452502	39.0	38.0	39.0	35.0	39.0
10	37.20396925988809	39.0	37.0	39.0	33.0	39.0
11	36.96868902378577	39.0	37.0	39.0	33.0	39.0
12	36.59090016668947	39.0	35.0	39.0	32.0	39.0
13	36.45861536028767	39.0	35.0	39.0	32.0	39.0
14	37.491915052683446	40.0	36.0	41.0	32.0	41.0
15	37.66588939498055	40.0	36.0	41.0	32.0	41.0
16	37.74115402117203	40.0	36.0	41.0	33.0	41.0
17	37.67829511532619	40.0	36.0	41.0	33.0	41.0
18	37.5441867347674	39.0	36.0	41.0	32.0	41.0
19	37.456215602463736	39.0	36.0	41.0	32.0	41.0
20	37.26908333361542	39.0	35.0	41.0	32.0	41.0
21	37.17050079862973	39.0	35.0	41.0	32.0	41.0
22	37.114247331977055	39.0	35.0	41.0	32.0	41.0
23	36.97254241828855	39.0	35.0	41.0	32.0	41.0
24	36.93514028851255	39.0	35.0	41.0	32.0	41.0
25	36.94651985548743	39.0	35.0	41.0	32.0	41.0
26	36.85277673136785	39.0	35.0	41.0	31.0	41.0
27	36.80422400899069	39.0	35.0	41.0	31.0	41.0
28	36.740665606985196	39.0	35.0	41.0	31.0	41.0
29	36.72988156681337	39.0	35.0	41.0	31.0	41.0
30	36.64062048912021	38.0	35.0	41.0	31.0	41.0
31	36.4612888234876	38.0	35.0	41.0	30.0	41.0
32	36.26057162835839	38.0	35.0	41.0	30.0	41.0
33	36.06915122802317	38.0	35.0	41.0	30.0	41.0
34	35.841445764022446	38.0	35.0	41.0	28.0	41.0
35	35.70586875585432	38.0	35.0	41.0	27.0	41.0
36	35.5829999463228	38.0	35.0	41.0	27.0	41.0
37	35.471500790408896	38.0	35.0	41.0	26.0	41.0
38	35.34499256981784	38.0	34.0	40.0	25.0	41.0
39	35.26924001301793	38.0	34.0	40.0	25.0	41.0
40	35.13400320225618	38.0	34.0	40.0	24.0	41.0
41	35.020043841218	38.0	34.0	40.0	24.0	41.0
42	35.01200966963374	38.0	34.0	40.0	24.0	41.0
43	34.96614878643582	38.0	34.0	40.0	24.0	41.0
44	34.84845547476035	38.0	34.0	40.0	23.0	41.0
45	34.758394074810546	38.0	34.0	40.0	23.0	41.0
46	34.703607059664385	38.0	34.0	40.0	23.0	41.0
47	34.64901879524178	38.0	34.0	40.0	23.0	41.0
48	34.503401732274554	38.0	34.0	40.0	23.0	41.0
49	34.360675742788516	37.0	33.0	40.0	23.0	41.0
50	34.28703618181968	37.0	33.0	40.0	23.0	41.0
51	33.99886431612101	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	6.0
12	8.0
13	8.0
14	42.0
15	93.0
16	277.0
17	757.0
18	1602.0
19	3221.0
20	5728.0
21	9215.0
22	14621.0
23	22152.0
24	34613.0
25	52749.0
26	70620.0
27	77941.0
28	81353.0
29	86416.0
30	97641.0
31	116217.0
32	141698.0
33	181732.0
34	286133.0
35	386002.0
36	299847.0
37	394666.0
38	633897.0
39	1136410.0
40	165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.195159186756527	24.4135040236141	29.472822168394575	14.918514621234799
2	32.971487733792024	25.04936126546665	29.045943333315606	12.933207667425723
3	28.239431273112025	24.407459293579	34.11365156338479	13.239457869924179
4	24.831315763640415	26.926153225685557	33.731286120284324	14.511244890389701
5	22.827197609962102	31.48228386342392	31.93747621398731	13.753042312626668
6	21.16487267138865	40.528270718795774	28.224029300982583	10.082827308832995
7	87.86979844935749	2.5533423246677693	8.222863877031815	1.3539953489429217
8	88.87547227475764	1.8586819490337378	8.000345274979605	1.2655005012290146
9	84.03763303846334	4.85839131841365	9.160570757917267	1.943404885205741
10	34.872918013634006	40.09276000922667	15.909463484269438	9.124858492869878
11	29.99985009069513	23.880334655597878	30.249811767106706	15.870003486600284
12	29.704141897377895	22.339339538289014	30.65739582391363	17.299122740419463
13	24.270606605584266	26.712387392724175	31.59911640554239	17.41788959614917
14	18.606694562692798	30.32399269409749	32.783786776742005	18.285525966467706
15	17.326178952056587	27.37203669199489	38.73973181708937	16.562052538859152
16	22.25853358717976	25.976501958250743	35.681654534490505	16.083309920078996
17	22.106593253017408	24.378662199691767	31.41528407571484	22.099460471575988
18	22.126492504292965	25.589252373281905	33.84981118681262	18.434443935612503
19	23.74369957788441	26.770126654019478	30.56225177316111	18.923921994935
20	24.45047359250879	27.95073496663551	30.30474627366572	17.294045167189978
21	23.43128375075015	26.168579299846172	33.30827591242782	17.091861036975857
22	22.698227249933144	23.45360089403975	32.909444624711725	20.938727231315376
23	19.390865300686634	27.51793229612214	32.70967838651164	20.381524016679585
24	19.382741183519457	27.001954140325747	35.51885786518511	18.096446810969685
25	20.394919138437377	27.863110560046657	32.635062238958334	19.106908062557636
26	19.43893299392577	30.129884323210266	29.92598348966617	20.505199193197793
27	18.39677317803374	29.618669414681538	33.33160857036332	18.652948836921404
28	17.43437961968493	28.40201033213615	35.08109851604296	19.082511532135964
29	18.732255694981955	26.985391580029567	34.150451879838506	20.131900845149975
30	20.785988992788397	27.36263109206027	32.787099288801244	19.06428062635009
31	23.012819179880044	26.939596705283623	31.009392543317745	19.03819157151859
32	23.718722753379367	27.70831711330774	30.54994470280964	18.023015430503257
33	22.963929403356133	27.350565810910204	30.192072505811403	19.49343227992226
34	19.78590049794068	28.129852407035678	32.19570708108691	19.88854001393673
35	20.17049040169407	28.206281973599523	31.15490128472274	20.468326339983665
36	23.51237984890109	27.80116416664692	29.811762270922866	18.874693713529123
37	20.185408795420706	30.264077329989547	30.311588908065456	19.238924966524284
38	20.674475813100816	29.960873671428782	29.08796629651964	20.27668421895076
39	19.830462247759463	28.701369542394595	30.121784384963224	21.34638382488272
40	21.822224973246023	27.527047749015072	29.772810030576665	20.87791724716224
41	19.489080074296986	27.235498329961988	30.426898178215083	22.848523417525946
42	20.63368597482394	27.73667898663244	29.87419224272541	21.755442795818208
43	20.597054910811217	27.984488739151526	29.791669588286183	21.626786761751077
44	20.444171598763393	29.233426680084357	29.759874308301544	20.56252741285071
45	19.794169688628703	30.08022082124186	28.298040975532384	21.827568514597058
46	20.84902343759445	29.216380541385366	29.490013380614403	20.44458264040578
47	20.869962382436043	28.29177863521602	30.000357848018076	20.837901134329858
48	20.634048658626046	28.079536074223483	30.659426853205424	20.626988413945046
49	21.168378614809008	26.607741026356475	31.136839631377853	21.087040727456664
50	19.59568493319606	29.132842372300242	30.250778923912325	21.02069377059137
51	18.99034148856071	29.766571869180435	29.428284597495935	21.814802044762917
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1866.0
1	3452.5
2	5039.0
3	36537.5
4	68036.0
5	48900.0
6	29764.0
7	27301.0
8	24838.0
9	25517.5
10	26197.0
11	26822.5
12	27448.0
13	27457.0
14	27466.0
15	26396.0
16	25326.0
17	24087.5
18	22849.0
19	22675.0
20	22501.0
21	23248.5
22	23996.0
23	26694.5
24	29393.0
25	33285.0
26	44153.0
27	51129.0
28	62135.5
29	73142.0
30	85751.5
31	98361.0
32	113234.5
33	128108.0
34	139682.0
35	151256.0
36	162829.0
37	174402.0
38	191435.0
39	208468.0
40	231386.5
41	254305.0
42	276816.0
43	299327.0
44	311006.5
45	322686.0
46	332296.5
47	341907.0
48	339791.0
49	337675.0
50	321463.5
51	305252.0
52	279956.0
53	254660.0
54	225747.5
55	196835.0
56	182598.5
57	168362.0
58	153035.0
59	137708.0
60	118737.5
61	99767.0
62	82515.0
63	65263.0
64	54219.5
65	43176.0
66	33766.0
67	24356.0
68	18305.5
69	12255.0
70	9747.0
71	7239.0
72	5460.0
73	3681.0
74	2902.5
75	1697.0
76	1270.0
77	894.5
78	519.0
79	437.0
80	355.0
81	280.0
82	205.0
83	123.0
84	41.0
85	44.5
86	48.0
87	36.0
88	24.0
89	20.0
90	16.0
91	12.5
92	9.0
93	6.0
94	3.0
95	2.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4135834.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.652303499325882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.08125061912257	15.9190538189372
2	8.268538930334701	3.415287509705272
3	3.079401298570433	1.9079019064288445
4	1.603735575995532	1.3248333539250372
5	1.0076894343808334	1.04055540159485
6	0.7362245228274561	0.9122839373447399
7	0.5333098847194684	0.7709854318891871
8	0.44185698095600956	0.7300291579199492
9	0.341922842215852	0.6355344879714502
>10	4.214631254221224	21.2030628498826
>50	1.8568235786058536	27.89480139129733
>100	0.8300589123121378	22.148385171258315
>500	0.0025178810656443906	0.3602364948958134
>1k	0.0019183855738241406	0.6515605506044088
>5k	0.0	0.0
>10k+	1.1989909836400879E-4	1.0854885363449802
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43837	1.0599313221952333	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.67156805616473E-5	0.0	0.0	0.035470475845984145	0.0
2	9.67156805616473E-5	0.0	0.0	0.10288130519745231	0.0
3	9.67156805616473E-5	0.0	0.0	0.1725649530421192	0.0
4	9.67156805616473E-5	0.0	0.0	0.27733221401052366	0.0
5	9.67156805616473E-5	0.0	0.0	0.5318637063286389	0.0
6	9.67156805616473E-5	0.0	0.0	0.9101670908455223	0.0
7	9.67156805616473E-5	0.0	0.0	1.159427578573028	0.0
8	9.67156805616473E-5	0.0	0.0	1.631351741873586	0.0
9	9.67156805616473E-5	0.0	0.0	1.8861492023132456	0.0
10	9.67156805616473E-5	0.0	0.0	2.241917833259265	0.0
11	9.67156805616473E-5	0.0	0.0	2.607865789584398	0.0
12	9.67156805616473E-5	0.0	0.0	2.888534694574299	0.0
13	9.67156805616473E-5	0.0	0.0	3.0275151275413856	0.0
14	9.67156805616473E-5	0.0	0.0	3.109844350619488	0.0
15	9.67156805616473E-5	0.0	0.0	3.1936243089060152	0.0
16	9.67156805616473E-5	0.0	0.0	3.3434127191758662	0.0
17	9.67156805616473E-5	0.0	0.0	3.537617805743654	0.0
18	9.67156805616473E-5	0.0	0.0	3.7738942133557587	0.0
19	9.67156805616473E-5	0.0	0.0	3.922812182500555	0.0
20	9.67156805616473E-5	0.0	0.0	4.099753520088089	0.0
21	9.67156805616473E-5	0.0	0.0	4.302566302225863	0.0
22	9.67156805616473E-5	0.0	0.0	4.540100013685269	0.0
23	9.67156805616473E-5	0.0	0.0	4.786845893718171	0.0
24	9.67156805616473E-5	0.0	0.0	4.9724674636361135	0.0
25	9.67156805616473E-5	0.0	0.0	5.148199855216626	0.0
26	9.67156805616473E-5	0.0	0.0	5.3206922714983245	0.0
27	9.67156805616473E-5	0.0	0.0	5.510738583801961	0.0
28	1.2089460070205912E-4	0.0	0.0	5.703734724362728	0.0
29	1.2089460070205912E-4	0.0	0.0	5.927631524862941	0.0
30	1.2089460070205912E-4	0.0	0.0	6.2120481624746064	0.0
31	1.2089460070205912E-4	0.0	0.0	6.452725133552265	0.0
32	1.2089460070205912E-4	0.0	0.0	6.688711394122685	0.0
33	1.2089460070205912E-4	0.0	0.0	6.924165718450015	0.0
34	1.2089460070205912E-4	0.0	0.0	7.18101838710161	0.0
35	1.2089460070205912E-4	0.0	0.0	7.463258921900637	0.0
36	1.2089460070205912E-4	0.0	0.0	7.718709213184088	0.0
37	1.2089460070205912E-4	0.0	0.0	7.995920532593909	0.0
38	1.2089460070205912E-4	0.0	0.0	8.314888847086223	0.0
39	1.2089460070205912E-4	0.0	0.0	8.814135190145446	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCGA	20	7.0354174E-4	45.0	12
CGTTTTT	23180	0.0	43.28192	1
CGACGGT	615	0.0	40.2439	28
ACGGGAT	2530	0.0	38.952568	5
GGCGATA	1445	0.0	38.771626	8
TACGGGA	2190	0.0	38.732876	4
GCGTAAG	245	0.0	38.57143	1
GGGCGAT	6220	0.0	38.271706	7
ATACGCG	100	0.0	38.250004	1
CGGACGG	255	0.0	37.941177	2
TAAGGGA	6250	0.0	37.907997	4
CGTAAGG	630	0.0	37.857143	2
TCGATAG	220	0.0	37.840908	1
CGGGATT	2210	0.0	37.771492	6
AGGGATT	9140	0.0	37.61488	6
TTGCGAG	360	0.0	37.5	1
CGGTCTA	660	0.0	37.5	31
CTCACGA	740	0.0	37.39865	24
GACGGTC	680	0.0	37.38971	29
TCACGAC	730	0.0	37.294518	25
>>END_MODULE