FastQCFastQC Report
Thu 26 May 2016
SRR1547939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547939_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3648670
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT462261.2669274009433575No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA41900.11483636503164167No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38170.10461346189159337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT211500.042.6489371
CGACGGT5500.042.13636428
CGGTCTA5550.041.75675631
GCGATAA3000.039.7500049
CACGACC9000.039.027
CGTAAGG6700.038.6194042
TAGGGAC39600.038.5795445
CCGTCGA356.252025E-638.5714320
TACGGGA15700.038.4076464
ACGGGAT16200.038.3333365
TAAGGGA58350.038.2904854
GCGCGAC3500.037.928579
TTAGGGA57200.037.8015754
CGGGTAT2450.037.653066
GGCGATA9700.037.577328
GGGCGAT47850.037.523517
ATAGGGA55450.037.493244
ACGGGAG21900.037.397265
CACGACG6400.037.2656326
TAGGGAT61800.036.9538845
Produced by FastQC (version 0.11.5)