##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547939_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3648670
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.338757410234415	31.0	31.0	33.0	30.0	34.0
2	31.717113633186887	31.0	31.0	34.0	30.0	34.0
3	31.68409255975465	31.0	31.0	34.0	30.0	34.0
4	35.550430430814515	37.0	35.0	37.0	33.0	37.0
5	35.519362397805224	37.0	35.0	37.0	33.0	37.0
6	35.592403807414755	37.0	35.0	37.0	33.0	37.0
7	35.918302833635266	37.0	35.0	37.0	35.0	37.0
8	35.92791948847114	37.0	35.0	37.0	35.0	37.0
9	37.67433777239378	39.0	37.0	39.0	35.0	39.0
10	37.15095418330515	39.0	37.0	39.0	33.0	39.0
11	36.97116209468108	39.0	37.0	39.0	33.0	39.0
12	36.57195087525044	39.0	35.0	39.0	32.0	39.0
13	36.45989826429905	39.0	35.0	39.0	32.0	39.0
14	37.52236129877462	40.0	36.0	41.0	32.0	41.0
15	37.669352120087595	40.0	36.0	41.0	32.0	41.0
16	37.730752575596036	40.0	36.0	41.0	33.0	41.0
17	37.64919737877089	40.0	36.0	41.0	32.0	41.0
18	37.51447075235634	39.0	36.0	41.0	32.0	41.0
19	37.40778119150266	39.0	36.0	41.0	32.0	41.0
20	37.21836312957873	39.0	35.0	41.0	32.0	41.0
21	37.11191283399157	39.0	35.0	41.0	32.0	41.0
22	37.03519172739656	39.0	35.0	41.0	32.0	41.0
23	36.89209986104526	39.0	35.0	41.0	31.0	41.0
24	36.86301117941606	38.0	35.0	41.0	31.0	41.0
25	36.87583941545824	39.0	35.0	41.0	31.0	41.0
26	36.77691186103429	39.0	35.0	41.0	31.0	41.0
27	36.70435555969709	38.0	35.0	41.0	31.0	41.0
28	36.62967382635316	38.0	35.0	41.0	31.0	41.0
29	36.613428180679534	38.0	35.0	41.0	31.0	41.0
30	36.50937903400417	38.0	35.0	41.0	31.0	41.0
31	36.35724606500451	38.0	35.0	41.0	30.0	41.0
32	36.15287625353896	38.0	35.0	41.0	30.0	41.0
33	35.94822661408129	38.0	35.0	41.0	29.0	41.0
34	35.721542644306005	38.0	35.0	41.0	27.0	41.0
35	35.554645391334375	38.0	35.0	41.0	27.0	41.0
36	35.43938065103175	38.0	35.0	40.0	26.0	41.0
37	35.341286002844875	38.0	34.0	40.0	25.0	41.0
38	35.213635105394566	38.0	34.0	40.0	25.0	41.0
39	35.12126884590823	38.0	34.0	40.0	24.0	41.0
40	34.95629530760524	38.0	34.0	40.0	24.0	41.0
41	34.82150262972535	38.0	34.0	40.0	23.0	41.0
42	34.79717677948403	38.0	34.0	40.0	23.0	41.0
43	34.7274870021131	38.0	34.0	40.0	23.0	41.0
44	34.58855555586008	38.0	34.0	40.0	23.0	41.0
45	34.46333376271354	37.0	33.0	40.0	23.0	41.0
46	34.385488410845596	37.0	33.0	40.0	23.0	41.0
47	34.30886980735446	37.0	33.0	40.0	23.0	41.0
48	34.17310554256757	37.0	33.0	40.0	22.0	41.0
49	34.03684438439212	37.0	33.0	40.0	22.0	41.0
50	33.95216805027585	37.0	33.0	40.0	22.0	41.0
51	33.65667160910688	36.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	10.0
12	7.0
13	17.0
14	52.0
15	127.0
16	273.0
17	752.0
18	1650.0
19	3127.0
20	5368.0
21	9009.0
22	13495.0
23	20685.0
24	31978.0
25	48499.0
26	64533.0
27	72337.0
28	74660.0
29	79874.0
30	90077.0
31	106860.0
32	128830.0
33	163560.0
34	253266.0
35	336447.0
36	283870.0
37	358129.0
38	558085.0
39	942966.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.180547980497	24.089298292254437	27.8766235367956	15.853530190452961
2	33.848224147429065	25.06754516029128	28.00845239498228	13.075778297297372
3	28.46152159554029	25.020815804114925	33.22695667188318	13.290705928461605
4	24.81326072240022	26.976953245977302	33.10343220954485	15.106353822077633
5	22.722005552708247	31.640625214119115	31.45962775477092	14.177741478401721
6	21.38962964587096	39.40690717439506	28.7089542216753	10.49450895805869
7	85.93906820841568	2.929999150375342	9.2564687954789	1.874463845730088
8	86.22215766292923	2.5085579128833246	9.343185325063654	1.9260990991237903
9	80.48773936804369	5.8441295047236395	10.95818476321509	2.709946364017574
10	33.14517344676279	40.09214316449556	16.38388782761938	10.378795561122272
11	31.689136041352057	24.094670112671192	28.109009584314283	16.107184261662468
12	29.115102215327777	21.924070962843995	30.68646931621659	18.274357505611633
13	24.371921823568588	26.350916909449197	31.646983695428744	17.63017757155347
14	19.22530675561232	29.54443673996278	32.28993030337082	18.940326201054084
15	17.588052632877186	26.883439719130532	37.713824489471506	17.814683158520776
16	22.713317455401558	25.49624931824473	35.1039419843395	16.686491242014213
17	21.73945574688859	25.23459233090414	30.78688946931348	22.239062452893794
18	22.571457544803998	25.629393724288573	32.83407378579044	18.96507494511699
19	23.45635532947622	27.997982826619015	29.81971512907443	18.725946714830336
20	25.33399841586112	27.324860839703234	29.522922051048738	17.818218693386907
21	23.37333877823974	27.455045263068463	31.499395670203118	17.67222028848868
22	23.174773273548993	23.797164446222872	31.67578871205124	21.352273568176898
23	19.408195315005194	28.52181753899366	31.617986828077083	20.452000317924064
24	19.67656707786673	27.18343944505806	34.168560050648594	18.971433426426614
25	20.74764229157474	28.298338846757858	31.517429638745075	19.436589222922326
26	20.287474614037443	29.435438118547307	29.377581420079096	20.89950584733615
27	19.71518388892391	28.364911049779785	31.715172926025097	20.204732135271207
28	17.797690665365735	28.366418448366115	33.3787105986565	20.45718028761165
29	19.02789235529659	27.299728394181987	32.591711500354926	21.080667750166498
30	20.25982070178997	27.07959338608315	32.63660457098066	20.02398134114623
31	22.897137861193258	27.251107937960956	30.109354915626792	19.742399285218998
32	22.564660547541983	27.99765393965472	29.659793842687883	19.77789167011541
33	22.294233241153627	27.7741478401719	29.431820361940105	20.499798556734373
34	19.287987129556797	28.94287507502734	31.414816905886255	20.35432088952961
35	19.65708052523248	28.861475551365288	30.91016726642859	20.571276656973637
36	22.24204984281944	29.4496624797529	29.053819610981535	19.25446806644613
37	20.30411081298117	30.730129060726235	28.888279838955018	20.07748028733758
38	19.907939057245517	31.190269331016506	28.548868491806605	20.352923119931372
39	20.305508582579407	29.027590875579325	28.651837518876743	22.015063022964533
40	22.49468984588905	27.492922078455983	29.273872397339307	20.73851567831566
41	19.48405857476834	27.53110037356078	29.21146609586507	23.773374955805814
42	20.98085055650415	27.62088651481225	28.835959404385708	22.562303524297896
43	20.55096788692866	27.625216859842077	28.96967936261706	22.854135890612195
44	20.664269446126944	28.606423710557543	29.025891626263817	21.703415217051695
45	19.831719503271056	29.44963507250587	27.89180715164704	22.826838272576033
46	21.051314588603518	28.355455549556414	28.826777976632584	21.766451885207484
47	20.564178179994354	28.51003242277323	29.692189208670555	21.23360018856186
48	20.042042716935214	28.477034097356025	30.1726382490058	21.30828493670296
49	21.0732129789759	26.76583522214944	30.104997163349935	22.055954635524724
50	19.24873995181806	28.77766418996511	30.343166139990736	21.630429718226093
51	19.232706712308868	28.59409044939663	29.231418571698732	22.941784266595775
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2268.0
1	3778.0
2	5288.0
3	36862.5
4	68437.0
5	48209.5
6	27982.0
7	26103.5
8	24225.0
9	25477.5
10	26730.0
11	27723.0
12	28716.0
13	28693.0
14	28670.0
15	27339.0
16	26008.0
17	24164.0
18	22320.0
19	21203.5
20	20087.0
21	20542.5
22	20998.0
23	22560.5
24	24123.0
25	27624.0
26	37166.0
27	43207.0
28	48468.5
29	53730.0
30	67197.0
31	80664.0
32	89085.5
33	97507.0
34	110253.5
35	123000.0
36	131934.0
37	140868.0
38	150080.0
39	159292.0
40	179530.5
41	199769.0
42	223531.5
43	247294.0
44	268249.0
45	289204.0
46	296354.0
47	303504.0
48	293029.0
49	282554.0
50	265325.0
51	248096.0
52	232784.0
53	217472.0
54	201330.0
55	185188.0
56	171239.5
57	157291.0
58	144128.5
59	130966.0
60	118902.5
61	106839.0
62	93871.5
63	80904.0
64	68331.0
65	55758.0
66	45684.0
67	35610.0
68	29122.0
69	22634.0
70	17516.0
71	12398.0
72	10049.5
73	7701.0
74	6254.0
75	3829.0
76	2851.0
77	2041.5
78	1232.0
79	978.5
80	725.0
81	498.5
82	272.0
83	231.0
84	190.0
85	131.0
86	72.0
87	57.0
88	42.0
89	30.5
90	19.0
91	20.5
92	22.0
93	14.0
94	6.0
95	5.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3648670.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.485500824461447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.74854622270702	17.25729499366896
2	8.033258146373797	3.6126366534679932
3	3.046863885291225	2.0553078121421295
4	1.6286290412600823	1.4648215859998175
5	1.0624406540821276	1.194475510165252
6	0.7445866945612863	1.0045442840664496
7	0.5708501436407988	0.8985095962835381
8	0.4404569529931983	0.7923116143734671
9	0.3760888491004369	0.7610891513866768
>10	5.050938843697203	28.551867223608777
>50	2.0187105984863116	31.60124845536036
>100	0.27339872838349283	8.137691792937474
>500	0.0031138329898759832	0.49840256538814026
>1k	0.001992853113520417	0.8751731718258483
>5k	0.0	0.0
>10k+	1.2455331959502607E-4	1.2946255893251517
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46226	1.2669274009433575	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	4190	0.11483636503164167	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3817	0.10461346189159337	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.481449404851631E-5	0.0	0.0	0.06690108998621415	0.0
2	5.481449404851631E-5	0.0	0.0	0.18280633765180188	0.0
3	5.481449404851631E-5	0.0	0.0	0.3057278405555997	0.0
4	5.481449404851631E-5	0.0	0.0	0.47315871262679277	0.0
5	5.481449404851631E-5	0.0	0.0	0.8971214168450422	0.0
6	5.481449404851631E-5	0.0	0.0	1.5327776970786615	0.0
7	5.481449404851631E-5	0.0	0.0	1.9533693099129272	0.0
8	5.481449404851631E-5	0.0	0.0	2.695091636130426	0.0
9	5.481449404851631E-5	0.0	0.0	3.0940589310625515	0.0
10	5.481449404851631E-5	0.0	0.0	3.650206787678798	0.0
11	5.481449404851631E-5	0.0	0.0	4.149484606719708	0.0
12	5.481449404851631E-5	0.0	0.0	4.543847484151759	0.0
13	5.481449404851631E-5	0.0	0.0	4.7461129671907845	0.0
14	5.481449404851631E-5	0.0	0.0	4.851110130540717	0.0
15	5.481449404851631E-5	0.0	0.0	4.976114584218359	0.0
16	5.481449404851631E-5	0.0	0.0	5.160839429161859	0.0
17	5.481449404851631E-5	0.0	0.0	5.381111473495822	0.0
18	5.481449404851631E-5	0.0	0.0	5.656472084348543	0.0
19	5.481449404851631E-5	0.0	0.0	5.823711105690567	0.0
20	5.481449404851631E-5	0.0	0.0	6.022797348074778	0.0
21	8.222174107277446E-5	0.0	0.0	6.257047088391112	0.0
22	8.222174107277446E-5	0.0	0.0	6.5102352364012095	0.0
23	8.222174107277446E-5	0.0	0.0	6.795133569218373	0.0
24	8.222174107277446E-5	0.0	0.0	7.009376019206999	0.0
25	8.222174107277446E-5	0.0	0.0	7.215533331323469	0.0
26	8.222174107277446E-5	0.0	0.0	7.400422619749114	0.0
27	8.222174107277446E-5	0.0	0.0	7.596494064960657	0.0
28	8.222174107277446E-5	0.0	0.0	7.812161691794544	0.0
29	8.222174107277446E-5	0.0	0.0	8.043889965384647	0.0
30	8.222174107277446E-5	0.0	0.0	8.33437937659476	0.0
31	8.222174107277446E-5	0.0	0.0	8.574521675021309	0.0
32	8.222174107277446E-5	0.0	0.0	8.82220096637953	0.0
33	8.222174107277446E-5	0.0	0.0	9.052778135594615	0.0
34	1.0962898809703262E-4	0.0	0.0	9.308926266283331	0.0
35	1.0962898809703262E-4	0.0	0.0	9.590590543951631	0.0
36	1.0962898809703262E-4	0.0	0.0	9.840791302036084	0.0
37	1.0962898809703262E-4	0.0	0.0	10.097980908111722	0.0
38	1.0962898809703262E-4	0.0	0.0	10.388854020780174	0.0
39	1.0962898809703262E-4	0.0	0.0	10.828356634061178	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	21150	0.0	42.648937	1
CGACGGT	550	0.0	42.136364	28
CGGTCTA	555	0.0	41.756756	31
GCGATAA	300	0.0	39.750004	9
CACGACC	900	0.0	39.0	27
CGTAAGG	670	0.0	38.619404	2
TAGGGAC	3960	0.0	38.579544	5
CCGTCGA	35	6.252025E-6	38.57143	20
TACGGGA	1570	0.0	38.407646	4
ACGGGAT	1620	0.0	38.333336	5
TAAGGGA	5835	0.0	38.290485	4
GCGCGAC	350	0.0	37.92857	9
TTAGGGA	5720	0.0	37.801575	4
CGGGTAT	245	0.0	37.65306	6
GGCGATA	970	0.0	37.57732	8
GGGCGAT	4785	0.0	37.52351	7
ATAGGGA	5545	0.0	37.49324	4
ACGGGAG	2190	0.0	37.39726	5
CACGACG	640	0.0	37.26563	26
TAGGGAT	6180	0.0	36.953884	5
>>END_MODULE