FastQCFastQC Report
Thu 26 May 2016
SRR1547938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547938_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1614809
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74990.46438928690637715No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37280.23086321664048193No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT21270.1317183642152106Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC16390.10149807190819471No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC207.0341356E-445.00000417
CAGCGAC207.0341356E-445.00000422
GGTACGA207.0341356E-445.0000048
GTACCGG207.0341356E-445.0000042
CGTGATT207.0341356E-445.00000440
TTCGTCC207.0341356E-445.00000416
ACCTCGC406.8175723E-945.00000419
ATATCCG406.8175723E-945.0000041
TCGTCCA207.0341356E-445.00000417
ATACGGT207.0341356E-445.00000428
AGTCGTC207.0341356E-445.00000420
GTACGTC207.0341356E-445.0000049
TGCCGGT207.0341356E-445.00000429
AGGTCGT207.0341356E-445.00000421
TCACGGC351.2123382E-745.018
TCTACGA253.8915103E-545.012
CTAGTCG351.2123382E-745.01
CGTAACA253.8915103E-545.037
GACGATT351.2123382E-745.09
ACTCGTT302.1660508E-644.99999630