##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547938_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1614809
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.317893942874978	31.0	31.0	33.0	30.0	34.0
2	31.70655786535745	31.0	31.0	34.0	30.0	34.0
3	31.820222701260644	31.0	31.0	34.0	30.0	34.0
4	35.59500101869633	37.0	35.0	37.0	33.0	37.0
5	35.479008353309894	37.0	35.0	37.0	33.0	37.0
6	35.53647583088774	37.0	35.0	37.0	33.0	37.0
7	35.89564276642005	37.0	35.0	37.0	35.0	37.0
8	35.90936946722491	37.0	35.0	37.0	35.0	37.0
9	37.68421280783053	39.0	37.0	39.0	35.0	39.0
10	37.11709744000684	39.0	37.0	39.0	33.0	39.0
11	36.83635030520637	39.0	37.0	39.0	32.0	39.0
12	36.02348884604928	37.0	35.0	39.0	32.0	39.0
13	35.690559069215	37.0	35.0	39.0	31.0	39.0
14	36.56719091855445	38.0	35.0	41.0	31.0	41.0
15	36.83500401595483	38.0	35.0	41.0	31.0	41.0
16	37.00775261965966	38.0	35.0	41.0	32.0	41.0
17	36.94954449721298	38.0	35.0	41.0	32.0	41.0
18	36.8969227939651	38.0	35.0	41.0	32.0	41.0
19	36.84086724807702	38.0	35.0	40.0	32.0	41.0
20	36.689899548491496	38.0	35.0	40.0	31.0	41.0
21	36.519340058174066	38.0	35.0	40.0	31.0	41.0
22	36.45568980603898	38.0	35.0	40.0	31.0	41.0
23	36.360347260883486	38.0	35.0	40.0	31.0	41.0
24	36.31358817048951	38.0	35.0	40.0	31.0	41.0
25	36.36603833642245	38.0	35.0	40.0	31.0	41.0
26	36.25510571219259	38.0	35.0	40.0	31.0	41.0
27	36.16663209085409	38.0	35.0	40.0	31.0	41.0
28	36.13574794294558	37.0	35.0	40.0	31.0	41.0
29	36.142384021887416	37.0	35.0	40.0	31.0	41.0
30	36.049930363281355	37.0	35.0	40.0	30.0	41.0
31	35.87988362710389	37.0	35.0	40.0	30.0	41.0
32	35.71880637276607	37.0	34.0	40.0	30.0	41.0
33	35.57556714137709	37.0	34.0	40.0	29.0	41.0
34	35.45622361530063	37.0	34.0	40.0	29.0	41.0
35	35.384550742533634	37.0	34.0	40.0	29.0	41.0
36	35.22755260838898	37.0	34.0	40.0	27.0	41.0
37	35.11575362782843	37.0	34.0	40.0	27.0	41.0
38	35.01311981788558	37.0	34.0	40.0	27.0	41.0
39	34.95197946010953	36.0	34.0	40.0	27.0	41.0
40	34.75168828016193	36.0	34.0	40.0	25.0	41.0
41	34.67914347764968	36.0	34.0	40.0	25.0	41.0
42	34.66275949663397	36.0	34.0	40.0	25.0	41.0
43	34.57290800336139	36.0	33.0	40.0	25.0	41.0
44	34.439526284532725	36.0	33.0	40.0	25.0	41.0
45	34.29752125483571	35.0	33.0	40.0	24.0	41.0
46	34.20031037726443	35.0	33.0	40.0	24.0	41.0
47	34.12171532360793	35.0	33.0	40.0	23.0	41.0
48	33.994004244464826	35.0	33.0	40.0	23.0	41.0
49	33.863590678526066	35.0	33.0	40.0	24.0	41.0
50	33.726866768763365	35.0	33.0	39.0	23.0	41.0
51	33.34470330546832	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	4.0
12	6.0
13	5.0
14	12.0
15	33.0
16	110.0
17	294.0
18	609.0
19	1294.0
20	2326.0
21	3772.0
22	6013.0
23	8845.0
24	13116.0
25	18428.0
26	23826.0
27	28390.0
28	31732.0
29	36711.0
30	43728.0
31	52823.0
32	66565.0
33	87288.0
34	155036.0
35	231854.0
36	114408.0
37	142575.0
38	212165.0
39	332772.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.53604605869796	25.53428919457348	29.55742753477346	12.372237211955097
2	29.478718535752524	27.226068222309884	30.519460815489634	12.775752426447958
3	29.246369075228092	25.903373092421454	31.64281348444305	13.2074443479074
4	26.40349415937117	27.904972043133274	30.4406279628117	15.250905834683854
5	23.36889378248449	32.03189974789588	30.326868378860905	14.272338090758721
6	21.235266833414972	41.403100924010204	26.356367842884204	11.005264399690613
7	88.76845496897775	3.4881524688059087	5.962562755099829	1.7808298071165074
8	89.20441984160355	2.546864675636561	5.6600501978871804	2.5886652848727003
9	84.86285374926695	4.848932598220594	7.437969444064282	2.850244208448182
10	45.55845304305339	31.303082903303114	12.322014554043234	10.816449499600262
11	40.4636709356958	22.296568820213412	21.36828566102864	15.871474583062145
12	37.34522163302285	22.494486964092967	23.988719408920808	16.171571993963376
13	23.44141009865563	37.52827733806289	24.235064332685784	14.795248230595693
14	16.616763964035375	40.883906393883116	26.02611206650446	16.473217575577053
15	14.113309995175898	27.47303241435984	42.95696890468161	15.456688685782652
16	18.095019287110738	23.045388030411026	41.601204848375254	17.25838783410298
17	17.610813415085	23.392673684627717	26.823605763901487	32.17290713638579
18	21.43213222121006	26.151823528355365	32.66528734977325	19.75075690066132
19	25.490940414624884	28.745504886336406	25.574603559925663	20.188951139113044
20	28.26470498987806	26.718515935940413	24.899043787841162	20.117735286340366
21	21.36710905128718	29.822350507087837	29.778754019825254	19.03178642179973
22	22.449837720745922	24.775747472301678	26.859399470773326	25.915015336179074
23	17.0306209588874	32.588869643406746	26.085376041377028	24.29513335632883
24	18.80302871732818	27.34273836720008	34.8410864690499	19.013146446421835
25	18.76358132757496	27.73306316722287	32.3322448661111	21.17111063909106
26	17.517118123567556	34.02934960109833	26.21938569824667	22.234146577087447
27	18.160661725318597	34.1593340141156	28.501575108882847	19.178429151682955
28	14.585378208816028	31.440312755254645	34.53473444847038	19.43957458745895
29	17.008265373799624	27.732381972109394	33.75365136062531	21.505701293465666
30	19.807296095079977	29.771384727233997	31.55784987574382	18.863469301942214
31	26.588717303408636	28.460146060617696	25.486110122002042	19.465026513971623
32	28.33381533048181	28.244950331587205	26.430370402939296	16.990863934991694
33	25.58946599876518	28.502008596682334	25.626745949520963	20.281779455031522
34	18.75881296178062	29.452585414126375	29.18592849061406	22.60267313347894
35	19.92724836188057	27.9255936770231	29.6975679476644	22.44959001343193
36	27.910483530869595	26.769481715794253	27.316543318745435	18.003491434590714
37	19.698242950095025	31.89188318866194	28.99463651738379	19.415237343859243
38	20.741400376143556	32.99374724812656	24.032377823011885	22.232474552718
39	19.739176583732192	29.952025285962613	28.449370792459046	21.85942733784615
40	23.313469270978796	26.483132060819575	27.509073828545667	22.69432483965596
41	17.997360678569414	25.468089414909134	28.686240911463834	27.848308995057618
42	22.49801679331735	27.454825926781435	25.58525497442732	24.4619023054739
43	21.491086561940143	27.260066051155274	27.06035202924928	24.188495357655302
44	20.122070164335224	29.672859143093707	27.470803048533917	22.73426764403716
45	19.62777021926432	32.69742737376371	24.35712211165531	23.31768029531666
46	22.633698474556432	29.844458384861618	26.81066305674541	20.711180083836542
47	21.32877634444693	28.91307888425195	27.384415122779227	22.373729648521902
48	21.669993169470818	27.306201538386272	29.23194012418806	21.79186516795485
49	21.665844071961452	24.19933255264245	30.709266544835952	23.425556830560147
50	20.212297553456786	29.655891192085253	28.3494208912633	21.782390363194658
51	18.099416091934092	30.68133754518336	26.093302675424773	25.125943687457774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1133.0
1	1744.5
2	2356.0
3	6502.5
4	10649.0
5	7579.0
6	4509.0
7	4743.5
8	4978.0
9	5891.5
10	6805.0
11	7726.0
12	8647.0
13	8723.0
14	8799.0
15	8363.0
16	7927.0
17	7725.0
18	7523.0
19	7487.5
20	7452.0
21	7094.5
22	6737.0
23	6835.5
24	6934.0
25	8112.5
26	12006.0
27	14721.0
28	16736.5
29	18752.0
30	21942.0
31	25132.0
32	30874.5
33	36617.0
34	38387.0
35	40157.0
36	43979.5
37	47802.0
38	56251.0
39	64700.0
40	83490.5
41	102281.0
42	125279.5
43	148278.0
44	151729.0
45	155180.0
46	158980.5
47	162781.0
48	166978.0
49	171175.0
50	157653.0
51	144131.0
52	124479.0
53	104827.0
54	93736.0
55	82645.0
56	71731.5
57	60818.0
58	53607.5
59	46397.0
60	40835.5
61	35274.0
62	30503.0
63	25732.0
64	20334.5
65	14937.0
66	11542.0
67	8147.0
68	6474.5
69	4802.0
70	3607.5
71	2413.0
72	2032.0
73	1651.0
74	1228.5
75	655.5
76	505.0
77	372.5
78	240.0
79	154.0
80	68.0
81	52.5
82	37.0
83	33.0
84	29.0
85	19.5
86	10.0
87	12.5
88	15.0
89	10.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1614809.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.823154563425543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.66070760552175	17.26816024058969
2	9.96305841201115	4.547768441235352
3	3.7917476176233915	2.5961892582755777
4	1.9412484379388113	1.7722165258034357
5	1.1354101124535827	1.2956820244702247
6	0.7236977354469067	0.9910239163983481
7	0.5043473667341524	0.8057558533241157
8	0.38444131740261367	0.7019330886117432
9	0.3216486917107095	0.6606934025433422
>10	3.3210961562332075	17.762364689010752
>50	1.458632888464076	24.52633157434063
>100	0.7824090937859919	24.298639406443844
>500	0.008528369163865129	1.2860336447227516
>1k	0.0027510868270532673	1.016357123675773
>5k	2.751086827053267E-4	0.47085081055443384
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7499	0.46438928690637715	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3728	0.23086321664048193	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	2127	0.1317183642152106	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	1639	0.10149807190819471	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.238536569959667E-4	0.0	0.0	0.16862675400000868	0.0
2	1.238536569959667E-4	0.0	0.0	0.5428505786133221	0.0
3	1.238536569959667E-4	0.0	0.0	0.8241841604796605	0.0
4	1.238536569959667E-4	0.0	0.0	1.2994725692016826	0.0
5	1.238536569959667E-4	0.0	0.0	2.1855835581793266	0.0
6	1.238536569959667E-4	0.0	0.0	2.9808478897504287	0.0
7	1.238536569959667E-4	0.0	0.0	3.410620079526433	0.0
8	1.238536569959667E-4	0.0	0.0	4.39612362824334	0.0
9	1.238536569959667E-4	0.0	0.0	4.711888526754557	0.0
10	1.238536569959667E-4	0.0	0.0	5.396427688971265	0.0
11	1.238536569959667E-4	0.0	0.0	6.420821285984906	0.0
12	1.238536569959667E-4	0.0	0.0	7.2573908121641635	0.0
13	1.238536569959667E-4	0.0	0.0	7.563618979086691	0.0
14	1.238536569959667E-4	0.0	0.0	7.6767592947525065	0.0
15	1.238536569959667E-4	0.0	0.0	7.875792121545024	0.0
16	1.238536569959667E-4	0.0	0.0	8.310580384429366	0.0
17	1.238536569959667E-4	0.0	0.0	8.86965579210916	0.0
18	1.238536569959667E-4	0.0	0.0	9.503786515928509	0.0
19	1.8578048549395006E-4	0.0	0.0	9.842402414155481	0.0
20	1.8578048549395006E-4	0.0	0.0	10.190245409828655	0.0
21	1.8578048549395006E-4	0.0	0.0	10.642930526148913	0.0
22	1.8578048549395006E-4	0.0	0.0	11.132090544454483	0.0
23	1.8578048549395006E-4	0.0	0.0	11.638837782053482	0.0
24	1.8578048549395006E-4	0.0	0.0	11.994111997146412	0.0
25	1.8578048549395006E-4	0.0	0.0	12.279161188722629	0.0
26	1.8578048549395006E-4	0.0	0.0	12.556469526736599	0.0
27	1.8578048549395006E-4	0.0	0.0	12.828018669700256	0.0
28	1.8578048549395006E-4	0.0	0.0	13.124648178205597	0.0
29	1.8578048549395006E-4	0.0	0.0	13.474968247018687	0.0
30	1.8578048549395006E-4	0.0	0.0	13.863187534872546	0.0
31	1.8578048549395006E-4	0.0	0.0	14.215798896340063	0.0
32	1.8578048549395006E-4	0.0	0.0	14.503820575684182	0.0
33	1.8578048549395006E-4	0.0	0.0	14.817232254712478	0.0
34	1.8578048549395006E-4	0.0	0.0	15.142781592126376	0.0
35	1.8578048549395006E-4	0.0	0.0	15.54369587982232	0.0
36	1.8578048549395006E-4	0.0	0.0	15.874323217173053	0.0
37	1.8578048549395006E-4	0.0	0.0	16.19498033513561	0.0
38	1.8578048549395006E-4	0.0	0.0	16.539665062555386	0.0
39	1.8578048549395006E-4	0.0	0.0	17.01154749571002	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTC	20	7.0341356E-4	45.000004	17
CAGCGAC	20	7.0341356E-4	45.000004	22
GGTACGA	20	7.0341356E-4	45.000004	8
GTACCGG	20	7.0341356E-4	45.000004	2
CGTGATT	20	7.0341356E-4	45.000004	40
TTCGTCC	20	7.0341356E-4	45.000004	16
ACCTCGC	40	6.8175723E-9	45.000004	19
ATATCCG	40	6.8175723E-9	45.000004	1
TCGTCCA	20	7.0341356E-4	45.000004	17
ATACGGT	20	7.0341356E-4	45.000004	28
AGTCGTC	20	7.0341356E-4	45.000004	20
GTACGTC	20	7.0341356E-4	45.000004	9
TGCCGGT	20	7.0341356E-4	45.000004	29
AGGTCGT	20	7.0341356E-4	45.000004	21
TCACGGC	35	1.2123382E-7	45.0	18
TCTACGA	25	3.8915103E-5	45.0	12
CTAGTCG	35	1.2123382E-7	45.0	1
CGTAACA	25	3.8915103E-5	45.0	37
GACGATT	35	1.2123382E-7	45.0	9
ACTCGTT	30	2.1660508E-6	44.999996	30
>>END_MODULE