FastQCFastQC Report
Thu 26 May 2016
SRR1547937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547937_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1319299
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53890.4084745004733574No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25150.19063153993143328No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC900.045.06
AGCCGTA207.0336665E-445.022
ACTATCG207.0336665E-445.024
CACGCTA253.8911203E-545.044
TAGTTCG253.8911203E-545.01
ATATCGT207.0336665E-445.020
CCGGTAA207.0336665E-445.029
GCGTGAT207.0336665E-445.044
ATCGGTT207.0336665E-445.028
CGATACG453.8562575E-1045.010
AACGTAC302.165747E-644.9999961
ACACCGT302.165747E-644.99999613
TTCGCAA302.165747E-644.99999614
TAATGCG302.165747E-644.9999961
GCGTAAG1050.042.8571471
TTCGTAG1000.042.751
TCTAGCG650.041.538461
GTCGGAT556.184564E-1140.9090929
CGTAAGG2150.040.8139532
TTGTACG501.0822987E-940.51
Produced by FastQC (version 0.11.5)