##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547937_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1319299
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38425178826028	31.0	31.0	33.0	30.0	34.0
2	31.787604629428206	31.0	31.0	34.0	30.0	34.0
3	31.902692263088202	31.0	31.0	34.0	30.0	34.0
4	35.675850584287566	37.0	35.0	37.0	33.0	37.0
5	35.579548684566575	37.0	35.0	37.0	33.0	37.0
6	35.631656660089945	37.0	35.0	37.0	33.0	37.0
7	35.93182970653355	37.0	35.0	37.0	35.0	37.0
8	35.92178952610439	37.0	35.0	37.0	35.0	37.0
9	37.675869533744816	39.0	37.0	39.0	35.0	39.0
10	37.19824164196289	39.0	37.0	39.0	33.0	39.0
11	36.991174100791405	39.0	37.0	39.0	33.0	39.0
12	36.28627020864868	38.0	35.0	39.0	32.0	39.0
13	36.00268779101629	38.0	35.0	39.0	32.0	39.0
14	36.9932638469369	39.0	35.0	41.0	31.0	41.0
15	37.243740046797576	39.0	35.0	41.0	32.0	41.0
16	37.36329065662901	39.0	35.0	41.0	32.0	41.0
17	37.31747920676056	39.0	35.0	41.0	32.0	41.0
18	37.223153356441564	39.0	35.0	41.0	32.0	41.0
19	37.16479812385214	39.0	35.0	41.0	32.0	41.0
20	37.015631786274376	38.0	35.0	41.0	32.0	41.0
21	36.87914339357492	38.0	35.0	41.0	32.0	41.0
22	36.805785496691804	38.0	35.0	41.0	31.0	41.0
23	36.70247229778845	38.0	35.0	40.0	31.0	41.0
24	36.65760832078248	38.0	35.0	40.0	31.0	41.0
25	36.65868692388913	38.0	35.0	40.0	31.0	41.0
26	36.558307100968015	38.0	35.0	40.0	31.0	41.0
27	36.483868327043375	38.0	35.0	40.0	31.0	41.0
28	36.4458549578223	38.0	35.0	40.0	31.0	41.0
29	36.48289963078877	38.0	35.0	40.0	31.0	41.0
30	36.41070219866763	38.0	35.0	40.0	31.0	41.0
31	36.247416999482304	38.0	35.0	40.0	30.0	41.0
32	36.06973248672212	38.0	35.0	40.0	30.0	41.0
33	35.9422556979123	38.0	35.0	40.0	30.0	41.0
34	35.80031592535126	38.0	35.0	40.0	29.0	41.0
35	35.729354755821085	38.0	35.0	40.0	29.0	41.0
36	35.60407155618249	38.0	35.0	40.0	29.0	41.0
37	35.484447422456924	38.0	34.0	40.0	28.0	41.0
38	35.390859085014085	38.0	34.0	40.0	27.0	41.0
39	35.34963643571321	38.0	34.0	40.0	27.0	41.0
40	35.169286113307145	38.0	34.0	40.0	26.0	41.0
41	35.105164939865794	37.0	34.0	40.0	26.0	41.0
42	35.09566216604424	37.0	34.0	40.0	26.0	41.0
43	35.00994467516461	37.0	34.0	40.0	26.0	41.0
44	34.86230035799315	37.0	34.0	40.0	26.0	41.0
45	34.7334031178679	37.0	34.0	40.0	25.0	41.0
46	34.671565733014276	37.0	34.0	40.0	25.0	41.0
47	34.61183173791536	37.0	34.0	40.0	25.0	41.0
48	34.503576520561296	36.0	34.0	40.0	24.0	41.0
49	34.386899406427204	36.0	34.0	40.0	24.0	41.0
50	34.25986755087361	36.0	33.0	40.0	24.0	41.0
51	33.93963006111579	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	7.0
13	6.0
14	15.0
15	34.0
16	79.0
17	238.0
18	531.0
19	1015.0
20	1815.0
21	2901.0
22	4513.0
23	6649.0
24	9941.0
25	14174.0
26	18273.0
27	21409.0
28	23987.0
29	27725.0
30	32539.0
31	39478.0
32	49110.0
33	64721.0
34	111694.0
35	164542.0
36	93476.0
37	120474.0
38	187203.0
39	322699.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.693119603668315	24.134331944464446	29.996990826188757	13.175557625678486
2	29.639376668973448	26.9642438901265	30.151163610371874	13.245215830528181
3	28.876850509247713	26.071042273207212	31.410468741354308	13.641638476190765
4	26.4617042838659	28.478153928715173	30.509308352390168	14.550833435028753
5	23.693491771008695	33.126986376856195	29.188000597286894	13.99152125484822
6	22.332693346997157	41.54789778511164	25.78899855150349	10.330410316387718
7	88.86984678984825	3.843707908518084	5.744869055460514	1.541576246173157
8	88.80087076545954	3.5920591162427926	5.463128525072785	2.143941593224887
9	83.6506356784929	6.923449498559463	7.048061129433131	2.3778536935145103
10	38.58731038225603	39.722761860654785	11.807710003570078	9.882217753519104
11	39.341043993817934	22.77717181624484	23.72206755254116	14.159716637396071
12	36.377652071289376	22.839553429510673	25.00517320182915	15.7776212973708
13	24.372791914493984	34.40607474120726	26.031703199956947	15.189430144341806
14	17.8085483275588	38.25379993466227	28.145325661582397	15.792326076196526
15	16.858119349745586	27.55091908657552	40.64294750469757	14.948014058981324
16	19.803092399827484	25.19823027228854	38.36522274329019	16.63345458459379
17	20.025407432280325	23.96196768132167	27.545764834203617	28.466860052194388
18	22.253029828719644	26.216574104884486	32.2450786364577	19.28531742993817
19	26.038146015421827	27.36597238381898	27.089007116658166	19.50687448410103
20	27.22695916543558	27.7765692235043	26.448288068133152	18.548183542926964
21	23.301541197257027	27.749130409406813	30.25371807300695	18.695610320329205
22	23.15972345920068	25.254017474431496	28.03367545946749	23.55258360690033
23	19.130841454439064	29.909368535866392	28.086279152792503	22.873510856902037
24	19.305707045938792	27.71350542977748	34.519544091218144	18.46124343306559
25	18.985233824932788	28.07536426541671	32.721619587371784	20.217782322278723
26	18.627847061204474	33.51999812021384	27.295253009363307	20.55690180921838
27	18.81923657942589	33.89375721500585	29.758985643133208	17.52802056243505
28	16.301535891408996	31.07703409158955	34.52932201115896	18.092108005842498
29	18.270763488792156	27.688188954891952	33.60132919072932	20.43971836558657
30	20.022148125633386	30.20566224942185	30.838574121560008	18.93361550338475
31	25.640359008837272	29.221579035533264	26.70069483869843	18.437367116931036
32	26.481184325918537	29.383028411300245	27.01002577884164	17.125761483939577
33	25.82265278757886	28.83963377520941	26.044437235228706	19.293276201983023
34	20.21429562214479	30.17420615038744	28.580556795692257	21.03094143177551
35	20.43782341986161	29.245758542983815	29.130697438563963	21.185720598590617
36	27.672271410802253	27.133879431425324	27.04390740840401	18.149941749368416
37	21.124703346246758	31.065209630265773	28.969930243257974	18.8401567802295
38	21.105526495510116	32.54046277606516	25.303058669793582	21.050952058631136
39	20.795816566221912	30.58033091816184	27.96621539165875	20.657637123957496
40	22.433201268249274	28.542278892047975	26.714490043576173	22.310029796126578
41	18.525595789885386	27.158816917165858	28.161319003501102	26.154268289447653
42	22.437521744502195	26.79642749672364	26.872073730064223	23.89397702870994
43	22.787404523159648	26.426230899894566	27.584194333505902	23.202170243439888
44	20.20717062621892	30.93695970360017	28.191410741613538	20.664458928567367
45	19.531660374183563	34.03868266405114	25.28395761688594	21.145699344879365
46	22.43835552062118	31.36877993540509	26.89193276126185	19.30093178271188
47	21.75473490088297	29.227188074879155	27.566988226323218	21.45108879791465
48	22.74139524095751	27.388181147715567	29.185499268929938	20.684924342396986
49	22.587980435064377	25.824017148500833	29.836147833053765	21.75185458338102
50	20.6574855282995	31.140173683145367	27.68720358311497	20.515137205440162
51	19.73608711899274	31.706838252738766	26.023896023570092	22.533178604698403
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1505.0
1	2099.0
2	2693.0
3	5982.0
4	9271.0
5	7178.5
6	5086.0
7	5247.0
8	5408.0
9	5824.5
10	6241.0
11	6683.0
12	7125.0
13	7237.5
14	7350.0
15	7088.0
16	6826.0
17	6584.5
18	6343.0
19	6185.5
20	6028.0
21	6312.0
22	6596.0
23	7854.0
24	9112.0
25	9398.5
26	11972.5
27	14260.0
28	17826.0
29	21392.0
30	24307.5
31	27223.0
32	31572.0
33	35921.0
34	39042.0
35	42163.0
36	44764.0
37	47365.0
38	52586.5
39	57808.0
40	72134.0
41	86460.0
42	100524.5
43	114589.0
44	115603.5
45	116618.0
46	115467.0
47	114316.0
48	113990.5
49	113665.0
50	109573.5
51	105482.0
52	95532.5
53	85583.0
54	76542.0
55	67501.0
56	59031.5
57	50562.0
58	45377.0
59	40192.0
60	34939.5
61	29687.0
62	25819.5
63	21952.0
64	18255.5
65	14559.0
66	12539.5
67	10520.0
68	7975.5
69	5431.0
70	4201.0
71	2971.0
72	2327.5
73	1684.0
74	1291.5
75	689.5
76	480.0
77	434.0
78	388.0
79	275.0
80	162.0
81	137.5
82	113.0
83	73.5
84	34.0
85	24.0
86	14.0
87	14.5
88	15.0
89	11.5
90	8.0
91	8.5
92	9.0
93	6.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1319299.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.610115705756368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.72620519332496	16.796722875971138
2	8.27281369420098	3.575529222876979
3	2.716889203420231	1.7613687013689432
4	1.4091061394806614	1.2180378686347506
5	0.8439399460935146	0.9118819941895321
6	0.5775265538746431	0.7488249391426666
7	0.435959506597535	0.6594794766418033
8	0.34182942568290187	0.5909578752511805
9	0.2817042098545194	0.5478894512779353
>10	4.644670452986592	27.110498100819115
>50	2.469898969175683	37.031480761572475
>100	0.27447276406666266	7.796813621730974
>500	0.00355995802939892	0.4786653348305768
>1k	0.001067987408819676	0.3572680512621663
>5k	3.55995802939892E-4	0.4145817244297618
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5389	0.4084745004733574	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2515	0.19063153993143328	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.579782899858182E-5	0.0	0.0	0.07314490498363146	0.0
2	7.579782899858182E-5	0.0	0.0	0.2418708723344746	0.0
3	7.579782899858182E-5	0.0	0.0	0.37914074065090625	0.0
4	7.579782899858182E-5	0.0	0.0	0.6173733171934489	0.0
5	7.579782899858182E-5	0.0	0.0	0.9921177837624374	0.0
6	7.579782899858182E-5	0.0	0.0	1.3889194185700133	0.0
7	7.579782899858182E-5	0.0	0.0	1.586524358769316	0.0
8	7.579782899858182E-5	0.0	0.0	2.0800440233790822	0.0
9	7.579782899858182E-5	0.0	0.0	2.2283045769003085	0.0
10	7.579782899858182E-5	0.0	0.0	2.547640830471334	0.0
11	7.579782899858182E-5	0.0	0.0	3.0225142291474487	0.0
12	7.579782899858182E-5	0.0	0.0	3.4146163985571127	0.0
13	7.579782899858182E-5	0.0	0.0	3.579628272287025	0.0
14	7.579782899858182E-5	0.0	0.0	3.6448144052258056	0.0
15	7.579782899858182E-5	0.0	0.0	3.762376838002606	0.0
16	1.5159565799716365E-4	0.0	0.0	4.0192556804788	0.0
17	1.5159565799716365E-4	0.0	0.0	4.322522794302126	0.0
18	1.5159565799716365E-4	0.0	0.0	4.742291171296272	0.0
19	1.5159565799716365E-4	0.0	0.0	4.943079620313515	0.0
20	1.5159565799716365E-4	0.0	0.0	5.1586486459854815	0.0
21	1.5159565799716365E-4	0.0	0.0	5.421212325636569	0.0
22	1.5159565799716365E-4	0.0	0.0	5.703862429972281	0.0
23	1.5159565799716365E-4	0.0	0.0	5.997654815170784	0.0
24	1.5159565799716365E-4	0.0	0.0	6.215800967028702	0.0
25	1.5159565799716365E-4	0.0	0.0	6.413026918083013	0.0
26	1.5159565799716365E-4	0.0	0.0	6.604264840646434	0.0
27	1.5159565799716365E-4	0.0	0.0	6.806114459269658	0.0
28	1.5159565799716365E-4	0.0	0.0	7.005765940851922	0.0
29	1.5159565799716365E-4	0.0	0.0	7.239071658509557	0.0
30	1.5159565799716365E-4	0.0	0.0	7.5277855891651555	0.0
31	2.2739348699574546E-4	0.0	0.0	7.800202986586058	0.0
32	2.2739348699574546E-4	0.0	0.0	8.043893006816498	0.0
33	2.2739348699574546E-4	0.0	0.0	8.279700052831087	0.0
34	2.2739348699574546E-4	0.0	0.0	8.5355177257013	0.0
35	2.2739348699574546E-4	0.0	0.0	8.820214371419974	0.0
36	2.2739348699574546E-4	0.0	0.0	9.087856505613967	0.0
37	2.2739348699574546E-4	0.0	0.0	9.353982683227986	0.0
38	2.2739348699574546E-4	0.0	0.0	9.616470565050076	0.0
39	2.2739348699574546E-4	0.0	0.0	9.997809442741941	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	90	0.0	45.0	6
AGCCGTA	20	7.0336665E-4	45.0	22
ACTATCG	20	7.0336665E-4	45.0	24
CACGCTA	25	3.8911203E-5	45.0	44
TAGTTCG	25	3.8911203E-5	45.0	1
ATATCGT	20	7.0336665E-4	45.0	20
CCGGTAA	20	7.0336665E-4	45.0	29
GCGTGAT	20	7.0336665E-4	45.0	44
ATCGGTT	20	7.0336665E-4	45.0	28
CGATACG	45	3.8562575E-10	45.0	10
AACGTAC	30	2.165747E-6	44.999996	1
ACACCGT	30	2.165747E-6	44.999996	13
TTCGCAA	30	2.165747E-6	44.999996	14
TAATGCG	30	2.165747E-6	44.999996	1
GCGTAAG	105	0.0	42.857147	1
TTCGTAG	100	0.0	42.75	1
TCTAGCG	65	0.0	41.53846	1
GTCGGAT	55	6.184564E-11	40.909092	9
CGTAAGG	215	0.0	40.813953	2
TTGTACG	50	1.0822987E-9	40.5	1
>>END_MODULE