FastQCFastQC Report
Thu 26 May 2016
SRR1547936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547936_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2874165
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115860.403108381042842No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA207.035055E-445.00000412
CGACGGT7300.043.15068428
CGGTCTA7650.041.17646831
TACGGGA15150.040.99014
ACGGGAT15000.039.8999985
CGTTTTT64950.039.7690541
ATTGGGA30800.039.594164
GCGATCG403.4606455E-739.3750049
TAGGGTA16500.039.2727285
GCGTAAG1550.039.1935461
AGGGCGA20850.038.8489236
CGAGGGA20650.038.6803864
TTACGCA356.2515646E-638.57142615
CGTTTAG356.2515646E-638.57142630
TCACGAC8350.038.53293625
ACGGTCT8250.038.45454430
GACGGTC8200.038.41463529
CTAACGG2350.038.297872
GGGCGAT38800.038.2731977
CACGACG8300.038.22289326
Produced by FastQC (version 0.11.5)