##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547936_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2874165
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26075503668022	31.0	31.0	33.0	30.0	34.0
2	31.65137352935548	31.0	31.0	34.0	30.0	34.0
3	31.797158479071314	31.0	31.0	34.0	30.0	34.0
4	35.58110338132988	37.0	35.0	37.0	33.0	37.0
5	35.48104579938869	37.0	35.0	37.0	33.0	37.0
6	35.52054179213789	37.0	35.0	37.0	33.0	37.0
7	35.86143036325333	37.0	35.0	37.0	35.0	37.0
8	35.859330970908076	37.0	35.0	37.0	35.0	37.0
9	37.590861693744095	39.0	37.0	39.0	35.0	39.0
10	36.97339818695169	39.0	37.0	39.0	33.0	39.0
11	36.82693060419287	39.0	37.0	39.0	32.0	39.0
12	36.39936712053762	38.0	35.0	39.0	32.0	39.0
13	36.24099903798147	38.0	35.0	39.0	32.0	39.0
14	37.22479815876959	39.0	35.0	41.0	32.0	41.0
15	37.40357216791659	39.0	35.0	41.0	32.0	41.0
16	37.49108140973117	39.0	35.0	41.0	32.0	41.0
17	37.42684292655432	39.0	35.0	41.0	32.0	41.0
18	37.355227692216694	39.0	36.0	41.0	32.0	41.0
19	37.31844135601123	39.0	36.0	41.0	32.0	41.0
20	37.1784532203266	39.0	35.0	41.0	32.0	41.0
21	37.06499626848145	39.0	35.0	41.0	31.0	41.0
22	37.004193913710594	39.0	35.0	41.0	31.0	41.0
23	36.861104703453	39.0	35.0	41.0	31.0	41.0
24	36.83422907174779	39.0	35.0	41.0	31.0	41.0
25	36.859278085983235	39.0	35.0	41.0	31.0	41.0
26	36.75813531930143	39.0	35.0	41.0	31.0	41.0
27	36.69531742262535	39.0	35.0	41.0	31.0	41.0
28	36.632386797556855	38.0	35.0	41.0	31.0	41.0
29	36.62886125187663	38.0	35.0	41.0	31.0	41.0
30	36.536468156838595	38.0	35.0	41.0	30.0	41.0
31	36.41881137652153	38.0	35.0	40.0	30.0	41.0
32	36.325260379971226	38.0	35.0	41.0	30.0	41.0
33	36.24103557033086	38.0	35.0	41.0	30.0	41.0
34	36.09844493966074	38.0	35.0	41.0	30.0	41.0
35	36.055197596519335	38.0	35.0	41.0	29.0	41.0
36	35.94848242880976	38.0	35.0	40.0	29.0	41.0
37	35.853620790734006	38.0	35.0	40.0	29.0	41.0
38	35.70174746404608	38.0	35.0	40.0	27.0	41.0
39	35.584062849558045	38.0	35.0	40.0	27.0	41.0
40	35.371312015837646	38.0	34.0	40.0	26.0	41.0
41	35.24182780042204	38.0	34.0	40.0	25.0	41.0
42	35.167019290820114	38.0	34.0	40.0	25.0	41.0
43	35.05862467881976	38.0	34.0	40.0	24.0	41.0
44	34.90265068289399	38.0	34.0	40.0	24.0	41.0
45	34.755854308990614	38.0	34.0	40.0	24.0	41.0
46	34.61420969220626	38.0	34.0	40.0	23.0	41.0
47	34.51662865562694	37.0	33.0	40.0	23.0	41.0
48	34.393988514925205	37.0	33.0	40.0	23.0	41.0
49	34.221962552602236	37.0	33.0	40.0	23.0	41.0
50	34.11248797476832	37.0	33.0	40.0	23.0	41.0
51	33.78779715152053	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	4.0
13	15.0
14	34.0
15	80.0
16	199.0
17	507.0
18	990.0
19	2093.0
20	3683.0
21	6047.0
22	9639.0
23	14635.0
24	21243.0
25	30804.0
26	40293.0
27	48103.0
28	54460.0
29	63755.0
30	75926.0
31	90593.0
32	110217.0
33	138480.0
34	211037.0
35	278209.0
36	215983.0
37	281056.0
38	435098.0
39	740857.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.52434533160066	24.45131020661653	29.435436030986388	12.588908430796423
2	29.57846887704777	26.384080245914898	30.035923476905467	14.001527400131863
3	28.615789281408688	25.685303383765373	31.330316805054686	14.368590529771256
4	25.576019470002592	28.72879601553843	30.15842862187801	15.536755892580976
5	23.078702858047468	32.646838299123395	29.532090189672477	14.742368653156657
6	21.914434279173257	40.85896251606989	26.15163012561909	11.074973079137768
7	88.9741542326206	3.0978040578742	6.044746909102296	1.8832948004028995
8	88.52240563781133	2.6663744078715035	5.981389377436577	2.82983057688059
9	82.68944197706116	5.860902209859211	8.187108255789074	3.2625475572905525
10	32.866554286201385	39.47014176291201	16.672737995209044	10.990565955677562
11	32.600877124312625	23.219613348572544	27.345820438283813	16.833689088831015
12	31.036283581492363	22.287794889994135	28.40257953179445	18.273341996719047
13	24.56421256260514	28.343501503915054	29.73712365156489	17.35516228191492
14	18.514629466297166	31.83578534983204	30.456497800230675	19.19308738364012
15	17.185165082728375	27.772309522939704	37.230221646982685	17.81230374734923
16	21.35267112361329	26.712558256050016	34.24636372650839	17.688406893828297
17	20.930565920884845	25.868869741298777	28.470425323528747	24.730139014287627
18	21.619705201336735	26.208029114542832	32.207336739540004	19.964928944580425
19	23.795432760471304	27.973411408182898	27.74764148891939	20.48351434242641
20	24.728260207747294	28.048146157231752	27.619117204475042	19.604476430545915
21	23.055252569007	27.71431006918531	30.705718008534653	18.524719353273035
22	22.341793181671896	23.65375683024461	30.224813119636483	23.77963686844701
23	18.928454003162656	28.40271870264929	30.283299671382817	22.38552762280523
24	19.53297740387208	26.977957076229096	33.11156457614646	20.37750094375236
25	19.866048052216907	28.604586027594102	30.023746027107002	21.505619893081988
26	18.935029826053828	31.001490867782465	27.619569509753266	22.443909796410434
27	19.251608728100162	30.026529444203792	29.831759832855802	20.89010199484024
28	16.943877613150253	28.88926001116846	32.59687596223599	21.569986413445296
29	18.3681173488648	26.840317100792753	31.51085619649533	23.28070935384712
30	20.138161866141992	28.006777620630686	30.55148190865869	21.30357860456863
31	22.300146303361153	28.142886716663796	27.744649315540336	21.812317664434712
32	22.829204308033813	27.637696513596122	27.71298794606433	21.82011123230573
33	21.96902404698408	26.786527565397257	28.086661691308606	23.15778669631006
34	18.068621669250025	27.703037229943305	29.607346829426984	24.620994271379686
35	18.145130846698084	27.26753683243655	30.028790970594937	24.558541350270428
36	22.132967313985105	26.873996447663927	28.721489545659345	22.271546692691615
37	18.732292683266273	29.718370378875257	28.77079082098627	22.778546116872207
38	19.044418117957736	29.726894593734183	27.885420635210572	23.34326665309751
39	19.27885142293501	27.646638241019566	27.642463115374376	25.432047220671045
40	21.37650413250457	25.75172267423756	28.94611130537043	23.92566188788744
41	18.15041933918199	25.579116021522776	29.562951326733156	26.70751331256208
42	19.930901670572148	26.55240739484337	28.792153547204148	24.724537387380334
43	20.392635774216163	26.197904434853253	29.30635506312268	24.103104727807903
44	19.945584195757725	27.983953600436994	28.14139062997427	23.92907157383101
45	18.836218519117725	28.89604459034189	26.879424111002674	25.38831277953771
46	20.62310270983051	27.84471316017	28.450871818423785	23.08131231157571
47	19.479466210186263	27.205605802032938	29.5149373818135	23.7999906059673
48	19.406923402101132	26.694570423062004	30.443589703444307	23.45491647139256
49	20.325868556606874	25.151548362741877	30.390983120314946	24.131599960336306
50	18.890390774364032	27.21642633599671	29.850756654541406	24.042426235097846
51	17.935017648604028	27.654153467180905	28.508140625190272	25.9026882590248
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1553.0
1	2724.5
2	3896.0
3	11585.5
4	19275.0
5	14341.5
6	9408.0
7	9002.5
8	8597.0
9	9208.5
10	9820.0
11	10385.0
12	10950.0
13	10965.5
14	10981.0
15	10531.0
16	10081.0
17	10062.0
18	10043.0
19	9674.0
20	9305.0
21	11412.0
22	13519.0
23	14854.5
24	16190.0
25	19408.0
26	28996.5
27	35367.0
28	42829.5
29	50292.0
30	56695.0
31	63098.0
32	70548.5
33	77999.0
34	87511.5
35	97024.0
36	105325.5
37	113627.0
38	123491.5
39	133356.0
40	152784.0
41	172212.0
42	190874.5
43	209537.0
44	217349.0
45	225161.0
46	228325.0
47	231489.0
48	243530.0
49	255571.0
50	257955.5
51	260340.0
52	237329.5
53	214319.0
54	189069.0
55	163819.0
56	146350.5
57	128882.0
58	111913.0
59	94944.0
60	81239.0
61	67534.0
62	56923.0
63	46312.0
64	39111.5
65	31911.0
66	25588.0
67	19265.0
68	15494.0
69	11723.0
70	8790.5
71	5858.0
72	4818.0
73	3778.0
74	2877.5
75	1673.5
76	1370.0
77	959.5
78	549.0
79	431.0
80	313.0
81	218.0
82	123.0
83	98.5
84	74.0
85	59.0
86	44.0
87	29.0
88	14.0
89	13.0
90	12.0
91	8.5
92	5.0
93	4.5
94	4.0
95	11.0
96	18.0
97	9.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2874165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.215901007221458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.68630911768393	18.812439712102396
2	8.981461449713828	4.349883627328916
3	3.220582582243507	2.3396792699157123
4	1.5697914541405227	1.5205565782179649
5	0.9174236369871459	1.1108119987482898
6	0.6372343219229795	0.9258721954854456
7	0.44470149717050056	0.753819320327081
8	0.3490118855536169	0.6761309816728073
9	0.27085898313836415	0.5903184890335762
>10	3.5892412976692163	22.243582151263027
>50	1.9867666830886075	34.37080545485877
>100	0.3418318836871414	10.806005557304774
>500	0.003190138000382715	0.5408346912674268
>1k	0.0014500627274466886	0.5524225011699233
>5k	0.0	0.0
>10k+	1.4500627274466885E-4	0.40683747130401293
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11586	0.403108381042842	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4792713709894876E-5	0.0	0.0	0.151243926496913	0.0
2	3.4792713709894876E-5	0.0	0.0	0.4832360007167299	0.0
3	3.4792713709894876E-5	0.0	0.0	0.7337087467142631	0.0
4	3.4792713709894876E-5	0.0	0.0	1.1381044581643711	0.0
5	3.4792713709894876E-5	0.0	0.0	1.93941544761696	0.0
6	3.4792713709894876E-5	0.0	0.0	2.736620896851781	0.0
7	3.4792713709894876E-5	0.0	0.0	3.195154070834486	0.0
8	6.958542741978975E-5	0.0	0.0	4.290637454704235	0.0
9	6.958542741978975E-5	0.0	0.0	4.634598222440257	0.0
10	6.958542741978975E-5	0.0	0.0	5.294337659807283	0.0
11	6.958542741978975E-5	0.0	0.0	6.102572399288141	0.0
12	6.958542741978975E-5	0.0	0.0	6.776437678421385	0.0
13	6.958542741978975E-5	0.0	0.0	7.045420148112583	0.0
14	6.958542741978975E-5	0.0	0.0	7.157800613395542	0.0
15	6.958542741978975E-5	0.0	0.0	7.342619508622504	0.0
16	6.958542741978975E-5	0.0	0.0	7.728923008943467	0.0
17	6.958542741978975E-5	0.0	0.0	8.19114421057942	0.0
18	6.958542741978975E-5	0.0	0.0	8.748071178933708	0.0
19	6.958542741978975E-5	0.0	0.0	9.045896808290408	0.0
20	6.958542741978975E-5	0.0	0.0	9.390449052159497	0.0
21	6.958542741978975E-5	0.0	0.0	9.813424072730689	0.0
22	6.958542741978975E-5	0.0	0.0	10.265311838394803	0.0
23	6.958542741978975E-5	0.0	0.0	10.730733969692068	0.0
24	6.958542741978975E-5	0.0	0.0	11.072572381891785	0.0
25	6.958542741978975E-5	0.0	0.0	11.366918739877494	0.0
26	6.958542741978975E-5	0.0	0.0	11.655315543818814	0.0
27	6.958542741978975E-5	0.0	0.0	11.966362404385274	0.0
28	6.958542741978975E-5	0.0	0.0	12.283845916988064	0.0
29	6.958542741978975E-5	0.0	0.0	12.638070535268504	0.0
30	6.958542741978975E-5	0.0	0.0	13.05975822543243	0.0
31	6.958542741978975E-5	0.0	0.0	13.417914420362088	0.0
32	6.958542741978975E-5	0.0	0.0	13.7417997922875	0.0
33	6.958542741978975E-5	0.0	0.0	14.070590936846006	0.0
34	6.958542741978975E-5	0.0	0.0	14.417265536251398	0.0
35	6.958542741978975E-5	0.0	0.0	14.804438854415109	0.0
36	6.958542741978975E-5	0.0	0.0	15.142658824388997	0.0
37	6.958542741978975E-5	0.0	0.0	15.498240358504122	0.0
38	6.958542741978975E-5	0.0	0.0	15.886979348784777	0.0
39	6.958542741978975E-5	0.0	0.0	16.393839602110525	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCGA	20	7.035055E-4	45.000004	12
CGACGGT	730	0.0	43.150684	28
CGGTCTA	765	0.0	41.176468	31
TACGGGA	1515	0.0	40.9901	4
ACGGGAT	1500	0.0	39.899998	5
CGTTTTT	6495	0.0	39.769054	1
ATTGGGA	3080	0.0	39.59416	4
GCGATCG	40	3.4606455E-7	39.375004	9
TAGGGTA	1650	0.0	39.272728	5
GCGTAAG	155	0.0	39.193546	1
AGGGCGA	2085	0.0	38.848923	6
CGAGGGA	2065	0.0	38.680386	4
TTACGCA	35	6.2515646E-6	38.571426	15
CGTTTAG	35	6.2515646E-6	38.571426	30
TCACGAC	835	0.0	38.532936	25
ACGGTCT	825	0.0	38.454544	30
GACGGTC	820	0.0	38.414635	29
CTAACGG	235	0.0	38.29787	2
GGGCGAT	3880	0.0	38.273197	7
CACGACG	830	0.0	38.222893	26
>>END_MODULE