FastQCFastQC Report
Thu 26 May 2016
SRR1547935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547935_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2038923
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407531.9987513015449825No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT39880.1955934579187149No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32280.1583188771719187No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC32020.15704369414637043No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG25900.12702784754500293No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT24180.11859202137599115TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT24000.11770920235830387No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAT207.034572E-445.031
CGTTTTT170800.043.485071
GCGCGAC1650.042.2727289
CGCCGGT2050.041.7073228
CGACGGT2650.041.60377528
CGGTCTA2650.040.7547231
ACGCTAG501.0822987E-940.51
CGAGGGT2650.039.9056634
GGGCGAT26350.039.7058837
GTAAGCG1250.039.61
CGTTAGG2900.039.5689662
CACGACG2800.039.37526
CGGTTTT11800.039.2796631
TACGGGA8700.039.0517234
TATGGGA24400.038.9139374
GGCGATC3200.038.6718758
TATAGCG1050.038.571431
GCACCGA6000.038.2500049
ACGGGAT9400.038.058515
TCACGAC2900.038.0172425
Produced by FastQC (version 0.11.5)