##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547935_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2038923
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.274208981898777	31.0	31.0	33.0	30.0	34.0
2	31.62569405514578	31.0	31.0	34.0	30.0	34.0
3	31.48347632549145	31.0	31.0	34.0	30.0	34.0
4	35.43544263319409	37.0	35.0	37.0	33.0	37.0
5	35.44094406703931	37.0	35.0	37.0	33.0	37.0
6	35.52008683015494	37.0	35.0	37.0	33.0	37.0
7	35.87460291536267	37.0	35.0	37.0	35.0	37.0
8	35.88846366439537	37.0	35.0	37.0	35.0	37.0
9	37.631692319915956	39.0	37.0	39.0	35.0	39.0
10	37.08684633995497	39.0	37.0	39.0	33.0	39.0
11	36.90880577638293	39.0	37.0	39.0	33.0	39.0
12	36.59157800466227	39.0	35.0	39.0	32.0	39.0
13	36.520048084209165	39.0	35.0	39.0	32.0	39.0
14	37.62991343959531	40.0	36.0	41.0	32.0	41.0
15	37.7529921434012	40.0	36.0	41.0	33.0	41.0
16	37.78448769276721	40.0	36.0	41.0	33.0	41.0
17	37.70973548289955	40.0	36.0	41.0	32.0	41.0
18	37.53599081475858	39.0	36.0	41.0	32.0	41.0
19	37.39237234559618	39.0	36.0	41.0	32.0	41.0
20	37.16743300261952	39.0	35.0	41.0	32.0	41.0
21	37.06664695037527	39.0	35.0	41.0	32.0	41.0
22	37.00259646882202	39.0	35.0	41.0	32.0	41.0
23	36.858581712011684	38.0	35.0	41.0	31.0	41.0
24	36.82432293912031	38.0	35.0	41.0	31.0	41.0
25	36.82856831768537	38.0	35.0	41.0	31.0	41.0
26	36.731713752799884	38.0	35.0	41.0	31.0	41.0
27	36.64971065606695	38.0	35.0	41.0	31.0	41.0
28	36.55842177463298	38.0	35.0	40.0	31.0	41.0
29	36.51612003003547	38.0	35.0	40.0	31.0	41.0
30	36.38184080517018	38.0	35.0	40.0	30.0	41.0
31	36.208104965219384	38.0	35.0	40.0	30.0	41.0
32	35.92208239349892	38.0	35.0	40.0	29.0	41.0
33	35.63753756272307	38.0	35.0	40.0	27.0	41.0
34	35.35931911111896	38.0	34.0	40.0	25.0	41.0
35	35.145100624202094	38.0	34.0	40.0	24.0	41.0
36	35.01281902259183	38.0	34.0	40.0	24.0	41.0
37	34.90805439930787	38.0	34.0	40.0	23.0	41.0
38	34.776667387635534	38.0	34.0	40.0	23.0	41.0
39	34.71420205667404	38.0	34.0	40.0	23.0	41.0
40	34.56877724171045	38.0	34.0	40.0	22.0	41.0
41	34.44984239228259	38.0	34.0	40.0	21.0	41.0
42	34.42116450694803	38.0	33.0	40.0	22.0	41.0
43	34.34669087552595	38.0	33.0	40.0	21.0	41.0
44	34.19775930724211	38.0	33.0	40.0	21.0	41.0
45	34.091314385094485	37.0	33.0	40.0	20.0	41.0
46	34.03233864152791	37.0	33.0	40.0	20.0	41.0
47	33.95311446288065	37.0	33.0	40.0	20.0	41.0
48	33.79403243771344	37.0	33.0	40.0	20.0	41.0
49	33.64578848735337	37.0	33.0	40.0	19.0	41.0
50	33.55223125149895	37.0	33.0	40.0	19.0	41.0
51	33.2709405897133	36.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	1.0
13	12.0
14	27.0
15	44.0
16	113.0
17	362.0
18	849.0
19	1630.0
20	3105.0
21	5074.0
22	7982.0
23	12249.0
24	19598.0
25	30413.0
26	41931.0
27	46457.0
28	46750.0
29	48112.0
30	53076.0
31	61191.0
32	73591.0
33	92236.0
34	137782.0
35	188842.0
36	161377.0
37	196857.0
38	301114.0
39	508086.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.606060160192417	24.00536950144758	27.20911971663471	18.179450621725294
2	34.97807420878571	24.439471230644806	27.65847459663754	12.92397996393194
3	27.242029247794058	24.485868274574372	35.36298330049737	12.909119177134205
4	24.143236404709743	26.548673000402662	35.007207236369396	14.300883358518199
5	21.946880779705758	30.831277100704636	33.35148016869691	13.8703619508927
6	20.05426394228718	38.3883550286107	30.77590473009525	10.781476299006878
7	82.66064976460612	2.8771562241438247	12.332785495087357	2.1294085161627
8	82.70042566590303	2.4036709576575475	12.286731769664671	2.609171606774753
9	77.0934459025672	5.20009828718397	14.41054909871535	3.295906711533491
10	31.692810370965457	35.2852461814399	22.192059239117906	10.829884208476729
11	27.53394806964265	23.112986611068685	32.45384940971287	16.89921590957579
12	26.27965842751296	22.22408595125956	33.174573046652576	18.32168257457491
13	22.063363844539495	26.024916095409196	34.71832923558173	17.193390824469585
14	17.42091290352799	29.322343217473147	34.96046687393296	18.29627700506591
15	16.490961159396406	27.26993613785317	39.55701122602472	16.682091476725702
16	20.178447150775188	26.74034281824277	37.100322081804954	15.980887949177088
17	19.61290347894452	26.75083855545305	32.74110890896812	20.89514905663431
18	20.43387611989271	26.752996557496285	35.02476552572118	17.78836179688983
19	21.04787674669421	28.96087787523119	31.806644978746135	18.184600399328467
20	22.17209772021798	28.580873333617795	31.6111005663284	17.635928379835825
21	20.903290609797427	28.530699786112567	33.361779723903254	17.204229880186745
22	20.44309667407744	24.682835006520598	33.79622477160736	21.077843547794593
23	17.41061334832164	28.575331192006757	33.71981188107643	20.294243578595168
24	17.825587332135644	27.48235220260893	36.2343747164557	18.457685748799733
25	18.828764009234288	28.575968783519535	33.49925426315756	19.09601294408862
26	18.215302882943593	30.759572578268035	31.532676810257183	19.49244772853119
27	17.99150826195987	29.32101898894662	33.23617419588675	19.451298553206765
28	17.073670756570994	29.407142888672105	34.51263240446059	19.006553950296308
29	17.78968602541636	28.361198534716614	33.78415957836564	20.064955861501392
30	19.00900622534544	28.926006524032537	32.94641337608139	19.11857387454063
31	20.557863146376786	28.539920340297304	30.810334671785057	20.091881841540854
32	20.496212951641628	30.061360826279365	30.684827234770513	18.757598987308498
33	19.718988897569943	29.575614184547433	30.13801894431521	20.567377973567417
34	16.85272077464426	30.51409984584999	32.16139108735347	20.47178829215228
35	16.999317777081334	30.54749983201916	30.69444015296311	21.758742237936403
36	19.574353715172176	29.96542782635735	29.699257892524635	20.760960565945847
37	17.7325480167716	31.1656202809032	29.62309022949861	21.478741472826584
38	17.285792548320853	31.214616736384848	29.408221889693724	22.091368825600576
39	17.65481089771414	29.588120787298	29.6042077116203	23.15286060336756
40	20.21322041097187	28.140788053300685	29.89862785401901	21.747363681708432
41	17.516747812448042	27.89340254634432	30.127719389108858	24.46213025209878
42	19.145009399570263	29.17040025542897	29.06004787821806	22.624542466782707
43	19.141281941495585	28.733257705170818	30.25048027806837	21.874980075265228
44	19.39705422911998	30.151751684590344	29.437600144782316	21.013593941507356
45	18.206768965772614	30.66702371791382	28.93414807719566	22.192059239117906
46	19.785445551401402	29.8726827839992	28.951853503050383	21.390018161549012
47	18.856376626287506	29.15328337558603	30.049638951544516	21.940701046581946
48	18.921558097093417	28.548552348470245	30.7670274944174	21.762862060018943
49	19.562582794936347	27.215740859267367	30.640195828876323	22.581480516919964
50	17.751136261644014	29.12934917110651	31.494274182987787	21.62524038426169
51	17.495707292526497	28.751502631536358	30.80057461708951	22.952215458847636
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1199.0
1	2009.0
2	2819.0
3	30429.0
4	58039.0
5	40152.5
6	22266.0
7	20110.0
8	17954.0
9	18812.5
10	19671.0
11	20604.5
12	21538.0
13	21425.5
14	21313.0
15	20437.5
16	19562.0
17	18393.5
18	17225.0
19	16460.5
20	15696.0
21	15677.5
22	15659.0
23	16337.0
24	17015.0
25	18998.0
26	23517.0
27	26053.0
28	30408.0
29	34763.0
30	40503.5
31	46244.0
32	49678.5
33	53113.0
34	59888.5
35	66664.0
36	71656.0
37	76648.0
38	82561.5
39	88475.0
40	98945.5
41	109416.0
42	122015.0
43	134614.0
44	143366.5
45	152119.0
46	163927.0
47	175735.0
48	180021.5
49	184308.0
50	172428.5
51	160549.0
52	141416.5
53	122284.0
54	107707.0
55	93130.0
56	83082.0
57	73034.0
58	65758.0
59	58482.0
60	49885.5
61	41289.0
62	35035.5
63	28782.0
64	23610.5
65	18439.0
66	14601.0
67	10763.0
68	8035.5
69	5308.0
70	4185.0
71	3062.0
72	2444.5
73	1827.0
74	1638.5
75	1099.0
76	748.0
77	532.5
78	317.0
79	254.5
80	192.0
81	141.5
82	91.0
83	72.0
84	53.0
85	35.0
86	17.0
87	12.0
88	7.0
89	6.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2038923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.08808463172194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9612807724874	22.25353275353149
2	8.152898044460276	4.90610172711042
3	3.389148326143384	3.059189449993826
4	2.0337840803167317	2.4477067012487437
5	1.390363709363772	2.0916690478106004
6	1.053428651962849	1.9017390260243374
7	0.8225538288968418	1.7324348452596567
8	0.7302737363240459	1.757803038627335
9	0.6087668649937834	1.64849660594289
>10	7.610900803864153	48.885635996847114
>50	0.2064859010386394	3.8909669816470136
>100	0.03646857600135579	1.8712660004462625
>500	0.001989111352804355	0.4336027500033231
>1k	0.0014918335146032664	1.087348419090459
>5k	0.0	0.0
>10k+	1.6575927940036295E-4	2.032506656416543
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40753	1.9987513015449825	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT	3988	0.1955934579187149	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3228	0.1583188771719187	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC	3202	0.15704369414637043	No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	2590	0.12702784754500293	No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	2418	0.11859202137599115	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT	2400	0.11770920235830387	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14679318444100145	0.0
2	0.0	0.0	0.0	0.362004842752767	0.0
3	0.0	0.0	0.0	0.6474496584716539	0.0
4	0.0	0.0	0.0	0.916317094858413	0.0
5	0.0	0.0	0.0	1.6495473345486809	0.0
6	0.0	0.0	0.0	2.873624948073076	0.0
7	0.0	0.0	0.0	3.6780692551901177	0.0
8	0.0	0.0	0.0	5.010341243882187	0.0
9	0.0	0.0	0.0	5.814883642001194	0.0
10	0.0	0.0	0.0	6.732426874384172	0.0
11	0.0	0.0	0.0	7.4845886774537345	0.0
12	0.0	0.0	0.0	8.05660635541411	0.0
13	0.0	0.0	0.0	8.378393887361122	0.0
14	0.0	0.0	0.0	8.609496287991258	0.0
15	0.0	0.0	0.0	8.794054508188882	0.0
16	0.0	0.0	0.0	9.065668492630667	0.0
17	0.0	0.0	0.0	9.391919165167101	0.0
18	0.0	0.0	0.0	9.78702972108314	0.0
19	0.0	0.0	0.0	10.019701577744721	0.0
20	0.0	0.0	0.0	10.282487372009635	0.0
21	0.0	0.0	0.0	10.569501643759965	0.0
22	0.0	0.0	0.0	10.889180219164725	0.0
23	0.0	0.0	0.0	11.222493443842657	0.0
24	0.0	0.0	0.0	11.488025786162597	0.0
25	0.0	0.0	0.0	11.725994556930301	0.0
26	0.0	0.0	0.0	11.962246735163614	0.0
27	0.0	0.0	0.0	12.216400521255585	0.0
28	0.0	0.0	0.0	12.476537858467436	0.0
29	0.0	0.0	0.0	12.752713074500607	0.0
30	0.0	0.0	0.0	13.085830117174606	0.0
31	0.0	0.0	0.0	13.369313112854188	0.0
32	0.0	0.0	0.0	13.659073932659545	0.0
33	0.0	0.0	0.0	13.931178372111159	0.0
34	0.0	0.0	0.0	14.207255497142365	0.0
35	0.0	0.0	0.0	14.512612786260197	0.0
36	0.0	0.0	0.0	14.789033229798282	0.0
37	0.0	0.0	0.0	15.083796690703867	0.0
38	0.0	0.0	0.0	15.38709406878043	0.0
39	0.0	0.0	0.0	15.782253670197452	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAT	20	7.034572E-4	45.0	31
CGTTTTT	17080	0.0	43.48507	1
GCGCGAC	165	0.0	42.272728	9
CGCCGGT	205	0.0	41.70732	28
CGACGGT	265	0.0	41.603775	28
CGGTCTA	265	0.0	40.75472	31
ACGCTAG	50	1.0822987E-9	40.5	1
CGAGGGT	265	0.0	39.905663	4
GGGCGAT	2635	0.0	39.705883	7
GTAAGCG	125	0.0	39.6	1
CGTTAGG	290	0.0	39.568966	2
CACGACG	280	0.0	39.375	26
CGGTTTT	1180	0.0	39.279663	1
TACGGGA	870	0.0	39.051723	4
TATGGGA	2440	0.0	38.913937	4
GGCGATC	320	0.0	38.671875	8
TATAGCG	105	0.0	38.57143	1
GCACCGA	600	0.0	38.250004	9
ACGGGAT	940	0.0	38.05851	5
TCACGAC	290	0.0	38.01724	25
>>END_MODULE