FastQCFastQC Report
Thu 26 May 2016
SRR1547934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547934_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2662733
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123970.4655742802601687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG750.045.01
CGACGGT10350.042.60869628
CGGTCTA10500.041.57142631
CGTTTTT68950.041.508341
TACGGGA11900.041.029414
CTCACGA11300.040.2212424
ACGGTCT11100.039.7297330
CACGACG11250.039.626
CGTAAGG4100.039.5121962
TTAGGGA41750.039.3413164
TCTCACG11700.038.84615323
ATAGGGC11650.038.626614
GTAGGGT17500.038.5714264
TCACGAC11800.038.5169525
TAAGGGA37100.038.510784
ACGACGG11600.038.4051727
TTGCGAG1700.038.382351
GCGCGAC5250.038.1428539
TAGGGAT45750.038.016395
TAGGGCG9950.037.9899525
Produced by FastQC (version 0.11.5)