##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547934_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2662733
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.326287314574913	31.0	31.0	33.0	30.0	34.0
2	31.718017540624615	31.0	31.0	34.0	30.0	34.0
3	31.84325052493059	31.0	31.0	34.0	30.0	34.0
4	35.63065091392941	37.0	35.0	37.0	33.0	37.0
5	35.54106588982072	37.0	35.0	37.0	33.0	37.0
6	35.59351425771942	37.0	35.0	37.0	33.0	37.0
7	35.90228047648788	37.0	35.0	37.0	35.0	37.0
8	35.91011791268595	37.0	35.0	37.0	35.0	37.0
9	37.67838795703512	39.0	37.0	39.0	35.0	39.0
10	37.09465913405512	39.0	37.0	39.0	33.0	39.0
11	36.91267768867551	39.0	37.0	39.0	33.0	39.0
12	36.41191137076079	38.0	35.0	39.0	32.0	39.0
13	36.24528707910256	39.0	35.0	39.0	32.0	39.0
14	37.27342884172014	39.0	35.0	41.0	32.0	41.0
15	37.4315761287369	39.0	35.0	41.0	32.0	41.0
16	37.49488439133777	39.0	35.0	41.0	32.0	41.0
17	37.42761666303005	39.0	35.0	41.0	32.0	41.0
18	37.348182487692156	39.0	35.0	41.0	32.0	41.0
19	37.309053517570106	39.0	35.0	41.0	32.0	41.0
20	37.18646706222516	39.0	35.0	41.0	32.0	41.0
21	37.086093874226215	39.0	35.0	41.0	32.0	41.0
22	37.00686550247434	39.0	35.0	41.0	31.0	41.0
23	36.888900238965	39.0	35.0	41.0	31.0	41.0
24	36.84291891075823	38.0	35.0	41.0	31.0	41.0
25	36.871439607350794	39.0	35.0	41.0	31.0	41.0
26	36.77908074147877	39.0	35.0	41.0	31.0	41.0
27	36.71242554172724	39.0	35.0	41.0	31.0	41.0
28	36.65291488106393	38.0	35.0	41.0	31.0	41.0
29	36.63334401158509	38.0	35.0	41.0	31.0	41.0
30	36.545422691648014	38.0	35.0	41.0	30.0	41.0
31	36.40349332809561	38.0	35.0	40.0	30.0	41.0
32	36.29358933096184	38.0	35.0	41.0	30.0	41.0
33	36.1522623560079	38.0	35.0	41.0	30.0	41.0
34	36.0103604078967	38.0	35.0	41.0	29.0	41.0
35	35.924044205708945	38.0	35.0	41.0	29.0	41.0
36	35.83861318427345	38.0	35.0	40.0	29.0	41.0
37	35.76868127596721	38.0	35.0	40.0	29.0	41.0
38	35.664452650716385	38.0	35.0	40.0	28.0	41.0
39	35.6024618315092	38.0	35.0	40.0	27.0	41.0
40	35.44742751150791	38.0	34.0	40.0	27.0	41.0
41	35.334682072892775	38.0	34.0	40.0	26.0	41.0
42	35.315185563103775	38.0	34.0	40.0	26.0	41.0
43	35.2358572939908	38.0	34.0	40.0	26.0	41.0
44	35.10931287515496	38.0	34.0	40.0	26.0	41.0
45	34.96959665126019	38.0	34.0	40.0	25.0	41.0
46	34.88512667248275	37.0	34.0	40.0	25.0	41.0
47	34.82132680971017	37.0	34.0	40.0	25.0	41.0
48	34.67554276001387	37.0	34.0	40.0	24.0	41.0
49	34.53595084448948	37.0	34.0	40.0	24.0	41.0
50	34.4361665251454	37.0	33.0	40.0	24.0	41.0
51	34.12863400123106	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	5.0
13	8.0
14	20.0
15	57.0
16	177.0
17	412.0
18	973.0
19	1923.0
20	3437.0
21	5558.0
22	8849.0
23	13144.0
24	19548.0
25	28037.0
26	36612.0
27	42910.0
28	48016.0
29	55189.0
30	65559.0
31	78951.0
32	97982.0
33	125519.0
34	202414.0
35	272004.0
36	195935.0
37	257388.0
38	405884.0
39	696094.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.09843683163126	24.791520591812997	29.112457013151527	12.997585563404215
2	29.907842806620117	25.819411859919867	30.534792635987163	13.73795269747286
3	28.850545661168432	25.978646751289	31.243650790372147	13.927156797170426
4	25.962347708163	28.071534021623645	31.219803112065687	14.746315158147663
5	22.76796058786217	32.586744521512294	30.43827526079408	14.207019629831455
6	21.29004297464297	42.06749230959319	26.56935562071	10.073109095053841
7	89.6204388498584	3.1096621403648057	5.651186206052203	1.6187128037245941
8	90.26646682187062	2.328622509279	5.431074013053506	1.9738366557968825
9	84.97093024347541	5.250582765902552	7.079380471117457	2.6991065195045842
10	36.47688296197929	36.612495507435405	14.347439266347772	12.563182264237533
11	34.54822545106851	23.2405577277181	24.639008116848366	17.57220870436503
12	33.29304890877155	22.551829267147703	26.136229205106183	18.018892618974565
13	25.860159467734846	27.808758895465672	27.55323196129691	18.777849675502576
14	20.062882759931245	31.743212706643888	28.26937586307001	19.924528670354857
15	18.402370797222254	27.29162105250508	36.12138355591792	18.184624594354748
16	22.346664122914316	24.92258893400127	34.60189211610778	18.128854826976646
17	22.571658517771027	24.806467640578308	27.896525862713233	24.72534797893743
18	22.937635880127676	26.512909856151555	31.082538129057625	19.466916134663144
19	24.77728709562694	28.208836560030615	26.68975822960845	20.324118114733995
20	26.241684765239327	27.84146964791438	26.251974944540063	19.66487064230623
21	23.849293188614855	28.017304025600765	29.432767010436265	18.70063577534811
22	22.99216631934182	24.450254681937693	29.044031076341486	23.513547922379
23	21.272129049363944	28.144654383297162	28.46868236507378	22.114534202265116
24	21.296953168042005	27.295601924789302	31.518631421175158	19.888813485993527
25	21.321439288129902	27.61606214366968	29.682773300965586	21.379725267234832
26	20.457815334845815	29.933718476467597	27.07511417780153	22.533352010885057
27	19.620292383802656	30.39651365720859	29.485983010688642	20.49721094830011
28	17.731330929537435	28.88648617792321	31.94413409080069	21.438048801738667
29	20.082449122762213	27.11556134242524	30.969571489142922	21.83241804566962
30	21.702964585634383	28.151414355100567	30.26450643004762	19.881114629217425
31	24.13182245459834	27.61178082819419	27.186278158568662	21.07011855863881
32	25.505373614252726	27.3224915904073	27.023625725898913	20.148509069441058
33	23.85590293882263	28.12377358150442	26.865217053305756	21.155106426367194
34	19.690934089148254	27.847929176526527	29.812301871798635	22.648834862526584
35	20.40414866980655	27.386110436157136	29.185802707218482	23.02393818681783
36	23.833031700887773	27.929537058353205	27.449691726508064	20.787739514250962
37	20.162367011638043	30.208623996472795	28.728190171526773	20.90081882036239
38	20.498900941250962	30.547411250020183	26.170404618112293	22.783283190616558
39	20.001179239525705	29.419697731616353	27.460657902989148	23.1184651258688
40	22.21356778918502	26.89567448181999	28.107324316782794	22.78343341221219
41	19.00971670835942	25.842433319450354	28.78726481400877	26.36058515818146
42	21.079019188179966	27.29424993042862	26.72911628766384	24.89761459372757
43	20.40854265147876	27.157510723005274	27.897840301675007	24.536106323840954
44	21.263904417003133	27.90948247533643	27.61024105683897	23.21637205082147
45	20.21772366962816	30.26938863190564	26.0127846088962	23.50010308957
46	21.27994057233677	29.33850295917766	27.728728340393122	21.65282812809245
47	20.873328268361867	27.439251325611693	28.425568767127608	23.261851638898833
48	20.49086408588469	27.60671084934164	29.121470308889403	22.780954755884274
49	21.166936377023156	25.617326258396915	29.67150668129324	23.54423068328668
50	20.192373775365386	28.205681906522358	28.74148478274014	22.860459535372115
51	19.41249836164572	28.63847032353601	27.790131417607398	24.158899897210873
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1656.0
1	2633.5
2	3611.0
3	11898.5
4	20186.0
5	14841.0
6	9496.0
7	9088.0
8	8680.0
9	9297.0
10	9914.0
11	10272.0
12	10630.0
13	10817.0
14	11004.0
15	10551.0
16	10098.0
17	9894.5
18	9691.0
19	9537.0
20	9383.0
21	9535.0
22	9687.0
23	11160.5
24	12634.0
25	15461.0
26	21954.0
27	25620.0
28	30502.0
29	35384.0
30	43542.5
31	51701.0
32	58208.5
33	64716.0
34	75083.0
35	85450.0
36	90424.0
37	95398.0
38	103132.0
39	110866.0
40	129695.0
41	148524.0
42	168769.0
43	189014.0
44	200496.0
45	211978.0
46	218464.5
47	224951.0
48	235910.0
49	246869.0
50	234839.0
51	222809.0
52	204165.0
53	185521.0
54	167759.0
55	149997.0
56	139406.0
57	128815.0
58	115864.0
59	102913.0
60	94252.0
61	85591.0
62	73936.5
63	62282.0
64	50263.0
65	38244.0
66	30506.5
67	22769.0
68	18035.0
69	13301.0
70	10129.0
71	6957.0
72	5365.5
73	3774.0
74	2893.0
75	1542.5
76	1073.0
77	829.5
78	586.0
79	488.0
80	390.0
81	264.5
82	139.0
83	99.5
84	60.0
85	49.0
86	38.0
87	27.5
88	17.0
89	12.5
90	8.0
91	6.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2662733.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.27895306710059
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35261283984399	17.010562780093863
2	9.212453274681408	4.104876282789685
3	3.2834282243553448	2.1945402992881844
4	1.6210140909888773	1.444579874169997
5	0.9428666553819601	1.0503040981894398
6	0.6662727106848357	0.8906315070742408
7	0.47226982477953067	0.7365174082889688
8	0.39678466730917505	0.7071957584580986
9	0.3101595166440603	0.6219026383164861
>10	4.2077268384179565	23.9126754182376
>50	2.0976226702221323	33.28872010213026
>100	0.43011380548656497	12.237843601303101
>500	0.004449920802763444	0.6707554977039145
>1k	0.0020538096012754354	0.6561896060477894
>5k	0.0	0.0
>10k+	1.7115080010628628E-4	0.47270512790832975
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12397	0.4655742802601687	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1266619672344166E-4	0.0	0.0	0.10451667516044605	0.0
2	1.1266619672344166E-4	0.0	0.0	0.30010519267234076	0.0
3	1.1266619672344166E-4	0.0	0.0	0.4731604708395472	0.0
4	1.1266619672344166E-4	0.0	0.0	0.7047270605051277	0.0
5	1.1266619672344166E-4	0.0	0.0	1.1796901904922499	0.0
6	1.1266619672344166E-4	0.0	0.0	1.6656194969604539	0.0
7	1.1266619672344166E-4	0.0	0.0	1.9466841023865329	0.0
8	1.1266619672344166E-4	0.0	0.0	2.6205030695905296	0.0
9	1.1266619672344166E-4	0.0	0.0	2.8562383085348775	0.0
10	1.1266619672344166E-4	0.0	0.0	3.3243288005218696	0.0
11	1.1266619672344166E-4	0.0	0.0	3.9465466496265305	0.0
12	1.1266619672344166E-4	0.0	0.0	4.443930352761617	0.0
13	1.1266619672344166E-4	0.0	0.0	4.658259014328511	0.0
14	1.1266619672344166E-4	0.0	0.0	4.757893487630942	0.0
15	1.1266619672344166E-4	0.0	0.0	4.902481773426025	0.0
16	1.1266619672344166E-4	0.0	0.0	5.213553142579448	0.0
17	1.1266619672344166E-4	0.0	0.0	5.585276480968989	0.0
18	1.1266619672344166E-4	0.0	0.0	6.022571545851575	0.0
19	1.1266619672344166E-4	0.0	0.0	6.271188286621302	0.0
20	1.1266619672344166E-4	0.0	0.0	6.5536048864080625	0.0
21	1.1266619672344166E-4	0.0	0.0	6.909292069463968	0.0
22	1.1266619672344166E-4	0.0	0.0	7.302947760815673	0.0
23	1.1266619672344166E-4	0.0	0.0	7.708020293435354	0.0
24	1.1266619672344166E-4	0.0	0.0	8.006961268741552	0.0
25	1.1266619672344166E-4	0.0	0.0	8.274355708965187	0.0
26	1.1266619672344166E-4	0.0	0.0	8.522183786357852	0.0
27	1.1266619672344166E-4	0.0	0.0	8.802422173007958	0.0
28	1.1266619672344166E-4	0.0	0.0	9.080632568117043	0.0
29	1.1266619672344166E-4	0.0	0.0	9.398651686068412	0.0
30	1.1266619672344166E-4	0.0	0.0	9.729927859834238	0.0
31	1.1266619672344166E-4	0.0	0.0	10.040060344014964	0.0
32	1.1266619672344166E-4	0.0	0.0	10.327847365845543	0.0
33	1.1266619672344166E-4	0.0	0.0	10.621455474506831	0.0
34	1.1266619672344166E-4	0.0	0.0	10.940901697616697	0.0
35	1.1266619672344166E-4	0.0	0.0	11.29441066753595	0.0
36	1.1266619672344166E-4	0.0	0.0	11.60413004232869	0.0
37	1.1266619672344166E-4	0.0	0.0	11.930937123624487	0.0
38	1.1266619672344166E-4	0.0	0.0	12.267621274833038	0.0
39	1.5022159563125555E-4	0.0	0.0	12.697818369322047	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	75	0.0	45.0	1
CGACGGT	1035	0.0	42.608696	28
CGGTCTA	1050	0.0	41.571426	31
CGTTTTT	6895	0.0	41.50834	1
TACGGGA	1190	0.0	41.02941	4
CTCACGA	1130	0.0	40.22124	24
ACGGTCT	1110	0.0	39.72973	30
CACGACG	1125	0.0	39.6	26
CGTAAGG	410	0.0	39.512196	2
TTAGGGA	4175	0.0	39.341316	4
TCTCACG	1170	0.0	38.846153	23
ATAGGGC	1165	0.0	38.62661	4
GTAGGGT	1750	0.0	38.571426	4
TCACGAC	1180	0.0	38.51695	25
TAAGGGA	3710	0.0	38.51078	4
ACGACGG	1160	0.0	38.40517	27
TTGCGAG	170	0.0	38.38235	1
GCGCGAC	525	0.0	38.142853	9
TAGGGAT	4575	0.0	38.01639	5
TAGGGCG	995	0.0	37.989952	5
>>END_MODULE