FastQCFastQC Report
Thu 26 May 2016
SRR1547933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547933_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2590567
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105990.4091382311285522No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC34730.13406331509665645No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT30940.1194333132476404TruSeq Adapter, Index 10 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACT207.0349267E-445.033
CCGTCGC207.0349267E-445.044
CGTCAAT207.0349267E-445.014
GCGATCG302.166562E-644.9999969
CGGTCTA8550.043.4210531
CGACGGT8750.042.68571528
CGTAAGG3300.040.909092
TAGGGTA13650.040.549455
TAACGCG451.9299478E-840.01
TACGGGA16250.039.8769264
CACGACG9400.039.73404326
CGTTTTT55750.039.4304961
TCTCACG9800.039.03061323
TTAGGGA46800.038.8942344
TCACGAC9800.038.8010225
TAGGGAC34850.038.737455
GACGTAG1050.038.571431
CGAGGGA21050.038.479814
TAAGGGA43450.038.4752584
GTAGGGT14800.038.4628374
Produced by FastQC (version 0.11.5)