##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547933_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2590567
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.373774544337206	31.0	31.0	33.0	30.0	34.0
2	31.778035078807072	31.0	31.0	34.0	30.0	34.0
3	31.912319581002922	31.0	31.0	34.0	30.0	34.0
4	35.67916984968927	37.0	35.0	37.0	33.0	37.0
5	35.58302371642964	37.0	35.0	37.0	33.0	37.0
6	35.634605474400004	37.0	35.0	37.0	33.0	37.0
7	35.94100596510339	37.0	35.0	37.0	35.0	37.0
8	35.93954180687085	37.0	35.0	37.0	35.0	37.0
9	37.67927445999273	39.0	37.0	39.0	35.0	39.0
10	37.15915010111686	39.0	37.0	39.0	33.0	39.0
11	36.9784332927888	39.0	37.0	39.0	33.0	39.0
12	36.47759158516263	39.0	35.0	39.0	32.0	39.0
13	36.319538541176506	39.0	35.0	39.0	32.0	39.0
14	37.30468078995834	39.0	35.0	41.0	32.0	41.0
15	37.48373193976454	39.0	35.0	41.0	32.0	41.0
16	37.57226352377684	40.0	35.0	41.0	33.0	41.0
17	37.50302385539536	39.0	35.0	41.0	32.0	41.0
18	37.43359426720097	39.0	36.0	41.0	32.0	41.0
19	37.39160577587841	39.0	36.0	41.0	32.0	41.0
20	37.25921583962121	39.0	35.0	41.0	32.0	41.0
21	37.161861862673305	39.0	35.0	41.0	32.0	41.0
22	37.092966134440836	39.0	35.0	41.0	32.0	41.0
23	36.97103105227543	39.0	35.0	41.0	31.0	41.0
24	36.94736827883626	39.0	35.0	41.0	31.0	41.0
25	36.9814476907951	39.0	35.0	41.0	32.0	41.0
26	36.890698831568535	39.0	35.0	41.0	31.0	41.0
27	36.82883399657295	39.0	35.0	41.0	31.0	41.0
28	36.785028914519486	39.0	35.0	41.0	31.0	41.0
29	36.79140473880815	39.0	35.0	41.0	31.0	41.0
30	36.712667921732965	38.0	35.0	41.0	31.0	41.0
31	36.57633020107181	38.0	35.0	41.0	31.0	41.0
32	36.49121485759681	38.0	35.0	41.0	30.0	41.0
33	36.406143133916245	38.0	35.0	41.0	30.0	41.0
34	36.27775000607975	38.0	35.0	41.0	30.0	41.0
35	36.23640230111786	38.0	35.0	41.0	30.0	41.0
36	36.1619830716596	38.0	35.0	41.0	30.0	41.0
37	36.045657572261206	38.0	35.0	41.0	30.0	41.0
38	35.92953704729505	38.0	35.0	40.0	29.0	41.0
39	35.866286029274676	38.0	35.0	40.0	29.0	41.0
40	35.70856920511996	38.0	35.0	40.0	28.0	41.0
41	35.60309808624907	38.0	35.0	40.0	28.0	41.0
42	35.60021261754666	38.0	35.0	40.0	28.0	41.0
43	35.54459081737704	38.0	34.0	40.0	28.0	41.0
44	35.4253555302758	38.0	34.0	40.0	27.0	41.0
45	35.319605707939616	38.0	34.0	40.0	27.0	41.0
46	35.26022797325837	38.0	34.0	40.0	27.0	41.0
47	35.189755756172296	38.0	34.0	40.0	27.0	41.0
48	35.050079384165706	37.0	34.0	40.0	26.0	41.0
49	34.9217422286318	37.0	34.0	40.0	26.0	41.0
50	34.82688809052227	37.0	34.0	40.0	26.0	41.0
51	34.49692287441321	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	0.0
11	2.0
12	6.0
13	11.0
14	20.0
15	48.0
16	173.0
17	411.0
18	797.0
19	1682.0
20	2802.0
21	4690.0
22	7107.0
23	10785.0
24	15960.0
25	23173.0
26	30908.0
27	36916.0
28	42318.0
29	50174.0
30	60395.0
31	74287.0
32	92380.0
33	120847.0
34	194602.0
35	268602.0
36	195660.0
37	255855.0
38	399794.0
39	700029.0
40	130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.78981010720819	24.599093557510766	30.208444714998684	12.402651620282356
2	30.20948695787447	25.85912659274977	30.456151105144162	13.47523534423159
3	29.217966568708704	25.51456881833205	31.603274495506195	13.664190117453051
4	26.43239877602085	27.920451391529344	30.287423564030576	15.35972626841923
5	23.282625000627274	32.990422560003275	29.246724751762837	14.480227687606611
6	22.314805986488672	41.352491558797745	25.634658358575557	10.698044096138027
7	89.443971146085	3.006330274414829	5.785413000320007	1.7642855791801564
8	89.13878699141925	2.5575482124183626	5.657101321834178	2.646563474328207
9	83.15832016697503	6.079634303995998	7.577916340322408	3.184129188706565
10	35.09054195471493	41.60158760611094	12.394892701095937	10.912977738078188
11	33.654138263939906	23.594603034779645	26.317211637452342	16.43404706382811
12	31.850710674535733	21.814992625166614	28.48851236042148	17.845784339876175
13	24.612488308544037	28.872289348239207	28.944358513020507	17.570863830196245
14	18.593381294519695	32.03622990642589	30.41851455685184	18.951874242202578
15	17.38638684118187	27.377327048480122	37.88259481418547	17.35369129615254
16	21.79009460091169	24.94284069858066	35.23456447951356	18.03250022099409
17	20.91414736619435	24.983372365972393	28.332793554461244	25.769686713372014
18	21.892852028146734	25.365142071214525	32.52315033735858	20.21885556328016
19	24.208484088618437	26.94977585987932	28.458055707495696	20.383684344006543
20	25.281878445915506	27.62677051008524	27.65062629146438	19.44072475253487
21	23.496439196515666	26.945182270908262	30.921377443625275	18.637001088950797
22	22.720470074697932	23.4698041008011	29.86442736281285	23.945298461688118
23	18.86864921849155	28.09751687564923	30.209293949934512	22.82453995592471
24	19.9592984856211	27.1165733215933	33.05125866267887	19.87286953010673
25	20.301462961583315	27.831088715327574	30.33501932202487	21.532429001064244
26	19.55764896256302	30.172583839754	27.964650209780327	22.305116987902647
27	19.35016542710534	30.077932745997305	30.033849732510298	20.53805209438706
28	17.462161758410417	28.497081912955736	33.72362112232573	20.31713520630812
29	18.602491269285835	25.959490721529306	33.40272612134718	22.035291887837683
30	21.083067915247895	27.42445958741851	31.54564232463395	19.946830172699645
31	23.359133348027672	28.02942367443112	27.988004170515566	20.623438807025643
32	23.567350313657204	27.61901159089883	29.402597964075046	19.411040131368924
33	24.2292517429582	26.58271335966219	28.347770970602188	20.840263926777418
34	19.009969632130726	28.31646508274057	30.74388734203748	21.92967794309122
35	19.11766806262876	27.388482907409845	30.55732586727153	22.936523162689866
36	24.535979961143642	25.91540770804229	29.16145384388823	20.387158486925834
37	20.098611616684686	30.22735949311483	29.033566782870313	20.640462107330173
38	20.262127943419337	29.492385257744736	27.62267874175808	22.622808057077854
39	19.52136346985042	27.888991097315763	29.25656815670083	23.333077276132986
40	21.671085905131964	26.040322446784813	29.554997033467963	22.733594614615257
41	18.619012748946467	26.041943713480485	29.092704415674252	26.2463391218988
42	20.988995845311084	26.320840186723604	28.564789098293925	24.12537486967139
43	21.089398575678604	25.675267229143273	29.753486398923478	23.481847796254645
44	20.32767343982997	27.894742733926588	29.040823881412837	22.736759944830613
45	19.28029655283959	28.932121809627002	28.076980830837417	23.710600806695986
46	21.58986816399653	27.953687358790564	28.939919330401416	21.51652514681149
47	20.34967634498548	27.405776418830317	29.843157887829193	22.401389348355014
48	20.37021238979729	26.816561779718494	30.337682831596325	22.47554299888789
49	21.24588941339869	24.581414030210375	30.874437912626853	23.298258643764086
50	19.852372086882912	27.290820889789764	30.591758483760508	22.26504853956682
51	18.90705779854372	27.911380018351196	28.672834943083892	24.5087272400212
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1884.0
1	3053.0
2	4222.0
3	10987.5
4	17753.0
5	12994.0
6	8235.0
7	8030.0
8	7825.0
9	8463.0
10	9101.0
11	9609.5
12	10118.0
13	10007.5
14	9897.0
15	9699.5
16	9502.0
17	9283.0
18	9064.0
19	9254.0
20	9444.0
21	10151.0
22	10858.0
23	12380.5
24	13903.0
25	17248.5
26	24914.5
27	29235.0
28	35988.5
29	42742.0
30	48759.5
31	54777.0
32	62186.5
33	69596.0
34	75924.5
35	82253.0
36	90632.0
37	99011.0
38	109978.5
39	120946.0
40	141270.0
41	161594.0
42	185487.5
43	209381.0
44	225867.5
45	242354.0
46	247019.5
47	251685.0
48	239809.0
49	227933.0
50	203704.5
51	179476.0
52	165413.5
53	151351.0
54	138510.0
55	125669.0
56	118128.0
57	110587.0
58	99200.5
59	87814.0
60	77410.0
61	67006.0
62	59106.5
63	51207.0
64	42609.5
65	34012.0
66	27249.5
67	20487.0
68	16362.0
69	12237.0
70	9748.5
71	7260.0
72	5813.0
73	4366.0
74	3384.0
75	1877.5
76	1353.0
77	965.0
78	577.0
79	511.5
80	446.0
81	345.0
82	244.0
83	150.5
84	57.0
85	58.0
86	59.0
87	38.5
88	18.0
89	18.5
90	19.0
91	11.5
92	4.0
93	4.5
94	5.0
95	4.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2590567.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.145376119373385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.53426688384201	15.76055107040595
2	10.071788873959248	4.2594352786957685
3	3.5784053003853096	2.2700017795261997
4	1.7986258544941847	1.5213048076523552
5	1.0714248211306812	1.13278404132203
6	0.7129521328584942	0.9045384602641393
7	0.5188329272958412	0.7679642173550247
8	0.39609910754445904	0.6700531687660571
9	0.31641318784551486	0.6021608279511019
>10	4.2045593282049	22.779209105105664
>50	2.339761106459278	35.30030724848357
>100	0.4500100808579116	12.232209600381465
>500	0.004820818193966812	0.7022745020143685
>1k	0.0018541608438333895	0.6816516576608872
>5k	0.0	0.0
>10k+	1.8541608438333895E-4	0.415554234415492
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10599	0.4091382311285522	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3473	0.13406331509665645	No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	3094	0.1194333132476404	TruSeq Adapter, Index 10 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.860158799212682E-5	0.0	0.0	0.14267146921890073	0.0
2	3.860158799212682E-5	0.0	0.0	0.42164514563800126	0.0
3	3.860158799212682E-5	0.0	0.0	0.6335292621267854	0.0
4	3.860158799212682E-5	0.0	0.0	0.987698831954549	0.0
5	3.860158799212682E-5	0.0	0.0	1.643385405588815	0.0
6	3.860158799212682E-5	0.0	0.0	2.304476201541979	0.0
7	3.860158799212682E-5	0.0	0.0	2.67130709223116	0.0
8	3.860158799212682E-5	0.0	0.0	3.6058129359325584	0.0
9	3.860158799212682E-5	0.0	0.0	3.9071369317991005	0.0
10	3.860158799212682E-5	0.0	0.0	4.488592651724507	0.0
11	3.860158799212682E-5	0.0	0.0	5.242481665210743	0.0
12	3.860158799212682E-5	0.0	0.0	5.887745810087135	0.0
13	3.860158799212682E-5	0.0	0.0	6.1578797228560385	0.0
14	3.860158799212682E-5	0.0	0.0	6.26044414215112	0.0
15	3.860158799212682E-5	0.0	0.0	6.427859229272974	0.0
16	3.860158799212682E-5	0.0	0.0	6.766433757551918	0.0
17	3.860158799212682E-5	0.0	0.0	7.206762071778109	0.0
18	3.860158799212682E-5	0.0	0.0	7.733403536754695	0.0
19	3.860158799212682E-5	0.0	0.0	8.023571673691512	0.0
20	3.860158799212682E-5	0.0	0.0	8.345354511193882	0.0
21	3.860158799212682E-5	0.0	0.0	8.730945773647237	0.0
22	3.860158799212682E-5	0.0	0.0	9.163939786154923	0.0
23	3.860158799212682E-5	0.0	0.0	9.600176332053948	0.0
24	3.860158799212682E-5	0.0	0.0	9.920337902860648	0.0
25	3.860158799212682E-5	0.0	0.0	10.19282651249707	0.0
26	3.860158799212682E-5	0.0	0.0	10.452924012388022	0.0
27	3.860158799212682E-5	0.0	0.0	10.745022228724446	0.0
28	3.860158799212682E-5	0.0	0.0	11.03407091960949	0.0
29	3.860158799212682E-5	0.0	0.0	11.372105025656545	0.0
30	3.860158799212682E-5	0.0	0.0	11.763447924720728	0.0
31	3.860158799212682E-5	0.0	0.0	12.103296305403411	0.0
32	3.860158799212682E-5	0.0	0.0	12.4063573727296	0.0
33	3.860158799212682E-5	0.0	0.0	12.712853981387086	0.0
34	3.860158799212682E-5	0.0	0.0	13.03780214910481	0.0
35	3.860158799212682E-5	0.0	0.0	13.41042327799281	0.0
36	3.860158799212682E-5	0.0	0.0	13.740775668029432	0.0
37	3.860158799212682E-5	0.0	0.0	14.082438323347747	0.0
38	3.860158799212682E-5	0.0	0.0	14.456719320519408	0.0
39	3.860158799212682E-5	0.0	0.0	14.983669598199931	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGACT	20	7.0349267E-4	45.0	33
CCGTCGC	20	7.0349267E-4	45.0	44
CGTCAAT	20	7.0349267E-4	45.0	14
GCGATCG	30	2.166562E-6	44.999996	9
CGGTCTA	855	0.0	43.42105	31
CGACGGT	875	0.0	42.685715	28
CGTAAGG	330	0.0	40.90909	2
TAGGGTA	1365	0.0	40.54945	5
TAACGCG	45	1.9299478E-8	40.0	1
TACGGGA	1625	0.0	39.876926	4
CACGACG	940	0.0	39.734043	26
CGTTTTT	5575	0.0	39.430496	1
TCTCACG	980	0.0	39.030613	23
TTAGGGA	4680	0.0	38.894234	4
TCACGAC	980	0.0	38.80102	25
TAGGGAC	3485	0.0	38.73745	5
GACGTAG	105	0.0	38.57143	1
CGAGGGA	2105	0.0	38.47981	4
TAAGGGA	4345	0.0	38.475258	4
GTAGGGT	1480	0.0	38.462837	4
>>END_MODULE