FastQCFastQC Report
Thu 26 May 2016
SRR1547932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547932_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2090578
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153130.7324768556829738No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGCG207.0346124E-445.0000041
GTCGTTA253.891906E-545.040
CGGTCTA4000.042.7531
CGTTTTT78750.042.3142851
CGCATCG700.041.78571721
TCACGAC4100.041.70731725
CACGACG4100.041.70731726
CGCGACC1300.041.53846410
TACGGGA10100.040.5445564
CTACGCG501.0822987E-940.51
ATAGCGG3900.040.3846172
GACCGAT9450.040.23819
GCGCGAC1350.040.0000049
CTCACGA4300.039.7674424
CGCGAGC403.460027E-739.37500424
GCGATAC1600.039.3750049
GACGGTC4350.039.31034529
TCTCACG4350.039.31034523
GGGCGAT29350.039.2504237
CGACGGT4400.038.86363628
Produced by FastQC (version 0.11.5)