FastQCFastQC Report
Thu 26 May 2016
SRR1547931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547931_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1580438
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388192.4562178332841906No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30320.1918455516761809No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC19740.12490208410579852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC351.21232E-745.00000440
CGTATCG207.034092E-445.015
ATAATCG207.034092E-445.018
TCTAGCG253.891473E-545.01
TATAGCG253.891473E-545.01
CGATCGA207.034092E-445.010
ACGCGCT406.8175723E-945.041
CGTTTTT109900.043.0959971
AAATGCG556.184564E-1140.9090921
CGGTCTA4500.040.00000431
TCGTTGA451.9290383E-840.024
ATTCACG451.9290383E-840.01
CACGACG4550.039.5604426
ACGGGAT6900.039.456525
ATACGAA6000.039.37500419
GACGTAG800.039.3751
GGACTAA7600.039.3758
CCTCGCG403.4593177E-739.37520
TAACGCC2250.039.00000412
TAGTACG750.039.0000041
Produced by FastQC (version 0.11.5)