##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547931_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1580438
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.357317401884792	31.0	31.0	33.0	30.0	34.0
2	31.723134979037457	31.0	31.0	34.0	30.0	34.0
3	31.63289353963901	31.0	31.0	34.0	30.0	34.0
4	35.52692291630548	37.0	35.0	37.0	33.0	37.0
5	35.51521223863258	37.0	35.0	37.0	33.0	37.0
6	35.60129154069948	37.0	35.0	37.0	33.0	37.0
7	35.92464114378419	37.0	35.0	37.0	35.0	37.0
8	35.94262413330988	37.0	35.0	37.0	35.0	37.0
9	37.70984246139361	39.0	37.0	39.0	35.0	39.0
10	37.16478533166122	39.0	37.0	39.0	33.0	39.0
11	36.994455967269836	39.0	37.0	39.0	33.0	39.0
12	36.52835036869526	39.0	35.0	39.0	32.0	39.0
13	36.360740503581916	39.0	35.0	39.0	32.0	39.0
14	37.4610842057708	40.0	35.0	41.0	32.0	41.0
15	37.628254319372225	40.0	35.0	41.0	33.0	41.0
16	37.718468551123166	40.0	35.0	41.0	33.0	41.0
17	37.66857795117556	40.0	35.0	41.0	33.0	41.0
18	37.52696847329664	39.0	36.0	41.0	33.0	41.0
19	37.38108233287228	39.0	36.0	41.0	32.0	41.0
20	37.15344923369344	39.0	35.0	41.0	32.0	41.0
21	37.035144055002476	39.0	35.0	41.0	32.0	41.0
22	36.94509243640054	38.0	35.0	41.0	32.0	41.0
23	36.79794462041535	38.0	35.0	41.0	32.0	41.0
24	36.68653373305375	38.0	35.0	41.0	31.0	41.0
25	36.62375430102288	38.0	35.0	40.0	31.0	41.0
26	36.48915047600728	38.0	35.0	40.0	31.0	41.0
27	36.3526946327537	38.0	35.0	40.0	31.0	41.0
28	36.24654557787145	38.0	35.0	40.0	31.0	41.0
29	36.194062658579455	38.0	35.0	40.0	31.0	41.0
30	36.07205027973258	37.0	35.0	40.0	30.0	41.0
31	35.90128179656526	37.0	35.0	40.0	30.0	41.0
32	35.67375816071241	37.0	35.0	40.0	30.0	41.0
33	35.4510344600674	37.0	34.0	40.0	29.0	41.0
34	35.21856535972939	37.0	34.0	40.0	27.0	41.0
35	35.06625505081503	37.0	34.0	40.0	26.0	41.0
36	34.909199221987826	37.0	34.0	40.0	25.0	41.0
37	34.76721136798786	37.0	34.0	40.0	24.0	41.0
38	34.644733928189524	37.0	34.0	40.0	24.0	41.0
39	34.552479122876065	36.0	34.0	40.0	24.0	41.0
40	34.422828987913476	36.0	34.0	40.0	23.0	41.0
41	34.32454863778269	36.0	34.0	40.0	23.0	41.0
42	34.2942836099866	36.0	33.0	40.0	23.0	41.0
43	34.24362360307712	36.0	33.0	40.0	23.0	41.0
44	34.11847665014382	36.0	33.0	40.0	23.0	41.0
45	33.99936030391575	35.0	33.0	40.0	23.0	41.0
46	33.93187331613135	35.0	33.0	40.0	23.0	41.0
47	33.85141207690526	35.0	33.0	40.0	23.0	41.0
48	33.69062247301065	35.0	33.0	40.0	22.0	41.0
49	33.54018126620595	35.0	33.0	40.0	21.0	41.0
50	33.43379620080003	35.0	33.0	40.0	20.0	41.0
51	33.14038133732547	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	5.0
14	25.0
15	49.0
16	119.0
17	298.0
18	642.0
19	1344.0
20	2410.0
21	3982.0
22	6377.0
23	9622.0
24	14376.0
25	21574.0
26	27740.0
27	30969.0
28	32139.0
29	35378.0
30	39845.0
31	47452.0
32	58786.0
33	76467.0
34	124265.0
35	188986.0
36	155103.0
37	153966.0
38	206342.0
39	342113.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.11351157084302	24.688092794529112	29.215698432966054	16.982697201661818
2	34.453170576764165	25.681804664276616	27.62575944137005	12.239265317589174
3	28.009450544722412	24.50656083946349	34.34832622348994	13.135662392324154
4	24.704543930226937	26.726704875483886	34.22861257448884	14.340138619800333
5	22.189165282029414	31.037155522709526	33.015594411169566	13.758084784091498
6	20.28602197618635	39.63008988647451	29.769468969994396	10.314419167344749
7	84.2534790988321	3.368370034129779	10.580231556062307	1.7979193109758183
8	84.49037545288078	2.844970824543576	10.396358477839687	2.268295244735953
9	79.3937503400956	5.563647545806922	12.102910712093735	2.9396914020037483
10	35.40075599295891	32.292124082058265	20.752917862010406	11.554202062972417
11	31.76821868368136	22.515024316044034	28.165230145061056	17.551526855213552
12	30.63340668852559	23.04253630955469	29.23803401335579	17.08602298856393
13	21.976439442736762	31.915456348176896	29.418996506031874	16.68910770305447
14	16.697269997304545	36.52158452277153	29.839386296710153	16.94175918321377
15	15.2472289327389	30.383349425918638	38.540391967290084	15.829029674052384
16	17.643083752731837	28.260456911311927	37.54338987040302	16.553069465553218
17	18.00019994457233	28.686920967478635	29.36242990867089	23.95044917927815
18	19.27737753711313	31.10504809426248	32.74440376655079	16.873170602073603
19	21.994852059998557	32.505609204536974	27.608991937678034	17.890546797786435
20	23.08246195042134	31.288161889299044	27.974460244565115	17.654915915714504
21	19.77413856158862	33.02476908300104	30.323049686226224	16.87804266918411
22	19.846207190664867	28.85092613566619	29.2509418275187	22.051924846150246
23	16.96453767879537	33.753111479222845	28.70849726468232	20.57385357729946
24	17.411439107386688	32.31756006879106	33.83100127939217	16.43999954443009
25	17.73641231101758	33.2058581228748	31.245135842089343	17.812593724018278
26	16.740992054101458	35.888152524806415	28.1569413036133	19.213914117478826
27	16.837104650736062	36.162823217361264	29.67765897808076	17.322413153821913
28	15.401110325112406	33.59056160380857	33.2626145410323	17.745713530046732
29	15.998792739734174	31.335743635625064	33.01141835364626	19.654045270994498
30	17.07368463679056	33.908448164369624	31.89191856940924	17.125948629430574
31	21.881149402887047	32.55357059245602	27.47086567141514	18.094414333241797
32	22.16777880562224	32.130713131423065	28.236792585346592	17.464715477608106
33	21.04600117182705	32.58058841915975	27.30508884246013	19.06832156655307
34	17.605562508621027	32.44176614330964	29.81977148107044	20.132899866998898
35	17.500971249742157	32.43031362192	29.645515989871164	20.42319913846668
36	23.03810715763605	31.569159941737666	28.368338397330362	17.024394503295923
37	18.115610988852456	35.05661088888017	29.185200558326237	17.642577563941135
38	18.57004197570547	35.23662427757369	27.616015307149034	18.577318439571815
39	17.515777271870203	34.644952854841506	28.50576865400604	19.33350121928225
40	20.232745605964926	32.69011501874797	28.30734264805073	18.769796727236372
41	17.532291681166868	31.66875258630835	29.16817996023887	21.630775772285908
42	18.817821388754258	32.96301405053536	27.492695063014178	20.726469497696208
43	18.58339270506024	32.905308528395295	27.93909030281479	20.572208463729673
44	18.37205888494202	34.083462938754955	28.15814350199122	19.386334674311804
45	17.32475427697891	36.44148014664289	25.815122137027835	20.418643439350355
46	19.966553575654345	34.403564075275334	27.411641582902966	18.218240766167355
47	19.011944789988597	33.807906415816376	27.92282898791348	19.25731980628155
48	19.115333850489545	32.57812074880508	29.424311488334247	18.882233912371127
49	19.12583726789662	31.135166327309268	29.214369687390455	20.524626717403656
50	18.043542359776215	33.21458987951441	29.678101893272625	19.06376586743675
51	17.296407704699586	33.91395296746851	27.96035023202429	20.829289095807617
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1875.0
1	2548.0
2	3221.0
3	25072.0
4	46923.0
5	30609.0
6	14295.0
7	14032.5
8	13770.0
9	17668.5
10	21567.0
11	25767.0
12	29967.0
13	31384.5
14	32802.0
15	30739.5
16	28677.0
17	24906.5
18	21136.0
19	19112.5
20	17089.0
21	15754.5
22	14420.0
23	13962.0
24	13504.0
25	13915.5
26	16274.0
27	18221.0
28	21714.0
29	25207.0
30	28681.0
31	32155.0
32	34909.5
33	37664.0
34	42112.5
35	46561.0
36	50105.0
37	53649.0
38	56465.0
39	59281.0
40	70300.0
41	81319.0
42	94754.0
43	108189.0
44	112213.0
45	116237.0
46	122356.0
47	128475.0
48	130489.0
49	132503.0
50	123090.5
51	113678.0
52	100771.5
53	87865.0
54	77973.5
55	68082.0
56	60960.5
57	53839.0
58	48701.5
59	43564.0
60	37272.5
61	30981.0
62	27539.5
63	24098.0
64	20918.0
65	17738.0
66	14368.5
67	10999.0
68	8505.0
69	6011.0
70	5098.5
71	4186.0
72	3414.0
73	2642.0
74	2055.0
75	1246.5
76	1025.0
77	805.5
78	586.0
79	429.0
80	272.0
81	213.5
82	155.0
83	113.5
84	72.0
85	63.5
86	55.0
87	55.0
88	55.0
89	35.5
90	16.0
91	11.5
92	7.0
93	7.0
94	7.0
95	5.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1580438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.012360809723678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.75963147049238	19.186621470026974
2	8.711197692942378	4.531976349472992
3	3.9596771258704875	3.0900165026445827
4	2.403623907461356	2.5009572932705066
5	1.54481706526591	2.009216944335765
6	1.0929623023859698	1.70583178566541
7	0.760224777342553	1.3842668843308434
8	0.558556351231386	1.1623495472634848
9	0.46745925141358163	1.0943746840452078
>10	5.403862320731548	34.685189863083096
>50	1.2473647972379707	21.28325902230069
>100	0.08420278417953046	3.2180903490982313
>500	0.003703934679760737	0.6741154368547404
>1k	0.002469289786507158	0.9803096519558883
>5k	0.0	0.0
>10k+	2.469289786507158E-4	2.4934242156516593
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38819	2.4562178332841906	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3032	0.1918455516761809	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	1974	0.12490208410579852	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.327359883779054E-5	0.0	0.0	0.047898114320207436	0.0
2	6.327359883779054E-5	0.0	0.0	0.2173448120078105	0.0
3	6.327359883779054E-5	0.0	0.0	0.43842276634705063	0.0
4	6.327359883779054E-5	0.0	0.0	0.6827854050585976	0.0
5	6.327359883779054E-5	0.0	0.0	1.3272902828203321	0.0
6	6.327359883779054E-5	0.0	0.0	2.1803449423514243	0.0
7	6.327359883779054E-5	0.0	0.0	2.7402530184670324	0.0
8	6.327359883779054E-5	0.0	0.0	3.6678439774290417	0.0
9	6.327359883779054E-5	0.0	0.0	4.180107033619794	0.0
10	6.327359883779054E-5	0.0	0.0	4.848149690149186	0.0
11	6.327359883779054E-5	0.0	0.0	5.565672300969731	0.0
12	6.327359883779054E-5	0.0	0.0	6.111976553335214	0.0
13	6.327359883779054E-5	0.0	0.0	6.334636347645399	0.0
14	6.327359883779054E-5	0.0	0.0	6.444099673634777	0.0
15	6.327359883779054E-5	0.0	0.0	6.5606496426939875	0.0
16	6.327359883779054E-5	0.0	0.0	6.806087932585777	0.0
17	6.327359883779054E-5	0.0	0.0	7.090629306559321	0.0
18	6.327359883779054E-5	0.0	0.0	7.437684996184602	0.0
19	6.327359883779054E-5	0.0	0.0	7.612636496971093	0.0
20	6.327359883779054E-5	0.0	0.0	7.790814951298311	0.0
21	6.327359883779054E-5	0.0	0.0	8.01765080313179	0.0
22	6.327359883779054E-5	0.0	0.0	8.265620036977092	0.0
23	6.327359883779054E-5	0.0	0.0	8.534089916845836	0.0
24	6.327359883779054E-5	0.0	0.0	8.725682374126666	0.0
25	6.327359883779054E-5	0.0	0.0	8.884878748802548	0.0
26	6.327359883779054E-5	0.0	0.0	9.043126019495862	0.0
27	6.327359883779054E-5	0.0	0.0	9.21073778281717	0.0
28	6.327359883779054E-5	0.0	0.0	9.37967829171407	0.0
29	6.327359883779054E-5	0.0	0.0	9.571840211384439	0.0
30	6.327359883779054E-5	0.0	0.0	9.811140962188963	0.0
31	6.327359883779054E-5	0.0	0.0	10.004947995429115	0.0
32	6.327359883779054E-5	0.0	0.0	10.182999902558658	0.0
33	6.327359883779054E-5	0.0	0.0	10.369530471932464	0.0
34	6.327359883779054E-5	0.0	0.0	10.562704769184238	0.0
35	6.327359883779054E-5	0.0	0.0	10.802258614384113	0.0
36	6.327359883779054E-5	0.0	0.0	10.994989996444025	0.0
37	6.327359883779054E-5	0.0	0.0	11.196263314347036	0.0
38	6.327359883779054E-5	0.0	0.0	11.415063419128115	0.0
39	6.327359883779054E-5	0.0	0.0	11.713524984845973	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCGC	35	1.21232E-7	45.000004	40
CGTATCG	20	7.034092E-4	45.0	15
ATAATCG	20	7.034092E-4	45.0	18
TCTAGCG	25	3.891473E-5	45.0	1
TATAGCG	25	3.891473E-5	45.0	1
CGATCGA	20	7.034092E-4	45.0	10
ACGCGCT	40	6.8175723E-9	45.0	41
CGTTTTT	10990	0.0	43.095997	1
AAATGCG	55	6.184564E-11	40.909092	1
CGGTCTA	450	0.0	40.000004	31
TCGTTGA	45	1.9290383E-8	40.0	24
ATTCACG	45	1.9290383E-8	40.0	1
CACGACG	455	0.0	39.56044	26
ACGGGAT	690	0.0	39.45652	5
ATACGAA	600	0.0	39.375004	19
GACGTAG	80	0.0	39.375	1
GGACTAA	760	0.0	39.375	8
CCTCGCG	40	3.4593177E-7	39.375	20
TAACGCC	225	0.0	39.000004	12
TAGTACG	75	0.0	39.000004	1
>>END_MODULE