FastQCFastQC Report
Thu 26 May 2016
SRR1547930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547930_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1088319
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308592.8354737903133183No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24730.22723117027268658No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT12300.11301833377897472No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTA207.0331217E-445.022
CGACGGT900.045.028
CTCGGAC207.0331217E-445.038
ACGCGAG302.165396E-644.9999961
CGTTTTT123650.044.0355831
CGGGATC3550.040.563386
ACGGGAT6500.040.4999965
CGTTAGG950.040.2631572
TGGGCGA4150.040.120486
TTACGGG3100.039.9193573
GGCGCGA850.039.7058838
GCGCGAC800.039.3759
CTAAGCG403.4579898E-739.3751
CGCGACC800.039.37510
CGTTCGG403.4579898E-739.37545
TCGGCGG403.4579898E-739.3752
GCGATCG403.4579898E-739.3759
GGCGATA3600.039.3758
CGGTTTT10700.039.322431
TACGGGA4600.039.1304324