##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547930_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1088319
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.323570570760964	31.0	31.0	33.0	30.0	34.0
2	31.64951728307601	31.0	31.0	34.0	30.0	34.0
3	31.351924389815853	31.0	31.0	34.0	28.0	34.0
4	35.36675184389871	37.0	35.0	37.0	33.0	37.0
5	35.41222288685579	37.0	35.0	37.0	33.0	37.0
6	35.544070258811985	37.0	35.0	37.0	33.0	37.0
7	35.93338258359911	37.0	35.0	37.0	35.0	37.0
8	35.97250530405148	37.0	35.0	37.0	35.0	37.0
9	37.752664430190045	39.0	38.0	39.0	35.0	39.0
10	37.21791680564246	39.0	37.0	39.0	34.0	39.0
11	36.9254345463049	39.0	37.0	39.0	33.0	39.0
12	36.6169753537336	39.0	35.0	39.0	32.0	39.0
13	36.51462484804547	39.0	35.0	39.0	32.0	39.0
14	37.666749363008456	40.0	36.0	41.0	33.0	41.0
15	37.81280488533233	40.0	36.0	41.0	33.0	41.0
16	37.87344611276657	40.0	36.0	41.0	33.0	41.0
17	37.808000227874366	40.0	36.0	41.0	33.0	41.0
18	37.57821833488159	39.0	36.0	41.0	33.0	41.0
19	37.373652394196924	39.0	36.0	41.0	33.0	41.0
20	37.07604388051665	38.0	35.0	41.0	32.0	41.0
21	36.957547373518246	38.0	35.0	41.0	32.0	41.0
22	36.88065172068116	38.0	35.0	41.0	32.0	41.0
23	36.74362847657718	38.0	35.0	41.0	32.0	41.0
24	36.676091293085946	38.0	35.0	40.0	32.0	41.0
25	36.64786703163319	38.0	35.0	40.0	31.0	41.0
26	36.5429446697154	38.0	35.0	40.0	31.0	41.0
27	36.463264906704744	38.0	35.0	40.0	31.0	41.0
28	36.3878954607978	38.0	35.0	40.0	31.0	41.0
29	36.344300705951106	38.0	35.0	40.0	31.0	41.0
30	36.20601588321071	38.0	35.0	40.0	31.0	41.0
31	35.97927813444404	38.0	35.0	40.0	30.0	41.0
32	35.58780651628796	38.0	35.0	40.0	29.0	41.0
33	35.2306511234298	38.0	34.0	40.0	25.0	41.0
34	34.86554585558095	38.0	34.0	40.0	23.0	41.0
35	34.56646075277561	38.0	34.0	40.0	22.0	41.0
36	34.384220067829375	38.0	34.0	40.0	21.0	41.0
37	34.23013564956598	38.0	33.0	40.0	20.0	41.0
38	34.089233028183834	37.0	33.0	40.0	18.0	41.0
39	33.99109268514103	37.0	33.0	40.0	18.0	41.0
40	33.86316328208917	37.0	33.0	40.0	18.0	41.0
41	33.72845277901057	37.0	33.0	40.0	18.0	41.0
42	33.69862145198237	37.0	33.0	40.0	18.0	41.0
43	33.619447055504864	37.0	33.0	40.0	18.0	41.0
44	33.45044605487913	37.0	33.0	40.0	16.0	41.0
45	33.35848129087152	36.0	33.0	40.0	17.0	41.0
46	33.31890282169107	36.0	33.0	40.0	16.0	41.0
47	33.26540196394623	36.0	33.0	40.0	16.0	41.0
48	33.12760688731888	36.0	33.0	40.0	15.0	41.0
49	32.99332732406583	36.0	32.0	40.0	15.0	41.0
50	32.888438959533005	35.0	32.0	40.0	15.0	41.0
51	32.64164918557886	35.0	31.0	40.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	1.0
13	1.0
14	10.0
15	26.0
16	77.0
17	263.0
18	558.0
19	1145.0
20	2033.0
21	3360.0
22	5055.0
23	7923.0
24	12900.0
25	20199.0
26	27471.0
27	28926.0
28	27072.0
29	26212.0
30	27583.0
31	31744.0
32	38201.0
33	47943.0
34	74862.0
35	109592.0
36	86407.0
37	103908.0
38	150007.0
39	254809.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.98332198555754	23.43669457208778	27.353928397831883	20.22605504452279
2	37.487170581419605	23.96273519069317	26.61250975127697	11.937584476610258
3	25.929989277041017	23.725212920108902	38.24540415080505	12.099393652045034
4	23.06180448930874	25.484347879619857	38.059796805899744	13.394050825171663
5	21.144627632155647	29.522226479552412	36.75310272080153	12.580043167490414
6	19.918700307538508	37.68068002120702	32.88842701450586	9.512192656748619
7	81.46618776296289	2.72364995924908	14.513851177825618	1.296311099962419
8	82.3460768396031	2.1098593335226163	14.105790673506574	1.4382731533677167
9	77.7085578768725	5.067080515914911	15.266939197055276	1.957422410157316
10	35.779491123466556	35.28184291554223	20.34311631056703	8.595549650424186
11	25.667658103919898	24.16690327008901	35.35764789551593	14.807790730475165
12	25.889100530267324	22.275454163714866	35.72987331839286	16.105571987624952
13	20.167800065973303	27.754270576917246	36.482869452798305	15.595059904311146
14	15.9709607201565	31.460628731098144	36.28099849400773	16.287412054737626
15	14.682735484724606	27.831913253375156	42.879982799160906	14.605368462739326
16	18.332676356840228	26.43949062728851	40.87220750533621	14.355625510535056
17	17.9508949122454	26.09859792946737	35.38769423303278	20.562812925254452
18	18.34783735283497	27.70327449948039	37.5307239880954	16.41816415958924
19	20.800794619959774	28.87765443771541	33.87958861326504	16.44196232905977
20	22.05805466963271	28.857531661213304	33.64629304459446	15.438120624559525
21	20.1773560876912	28.472718017419524	35.822309451548676	15.527616443340603
22	19.43841833138997	26.201417047758973	35.63082147789389	18.729343142957166
23	16.503065737159787	29.856595354854598	35.236084273085375	18.404254634900244
24	16.42018562572187	28.882248678925944	38.499925113868265	16.19764058148392
25	16.68490580427246	30.191056114980995	36.28320372978878	16.840834350957763
26	16.40640290209029	33.40224695149124	33.10141603702591	17.08993410939256
27	16.203704979881817	31.924922747834046	35.69900001745812	16.17237225482602
28	15.094655151660497	30.776729984499028	37.97691669446183	16.151698169378648
29	15.932920402933332	28.96476125106701	37.60423184746384	17.498086498535812
30	17.049596671564128	30.46854828409685	35.45715916013595	17.02469588420307
31	20.309118925609127	29.72134089361667	33.09535163862801	16.87418854214619
32	20.27447834688175	30.241868422769425	32.923986441475336	16.559666788873486
33	19.564759964679475	30.97547685926645	32.181373292205684	17.278389883848394
34	17.067881751582025	31.370949142668646	33.16003855487224	18.401130550877088
35	17.24476003818733	31.070853306796998	32.84928407939216	18.83510257562351
36	20.8924956745219	31.277318506798096	30.952046229092755	16.878139589587242
37	18.05481664842753	32.993359483754304	32.31083900951835	16.640984858299817
38	18.079074242019114	33.666875245217625	30.06995191667149	18.184098596091772
39	17.82850432639695	32.89375633431007	31.10475880693069	18.172980532362295
40	19.446136656623654	31.800510695852964	30.359940421879983	18.393412225643402
41	17.19036422225469	31.18653630047808	31.287425837461257	20.335673639805975
42	18.85210126810246	32.085721190202506	29.541430407812413	19.520747133882622
43	18.786495503616127	31.913253375159307	30.42122759962842	18.87902352159615
44	18.15396037375071	33.66108650129236	30.142908467094664	18.042044657862263
45	17.057590651270445	34.74560308144947	28.794498671804863	19.402307595475225
46	19.008121699611973	33.63480744156814	29.236464676257608	18.120606182562284
47	18.11196900908649	32.78303512113636	30.32814827270313	18.77684759707402
48	18.719327697118217	31.96379002847511	30.856577896737996	18.460304377668677
49	18.46232584380131	31.214836826334924	30.71498338262954	19.607853947234222
50	17.136152175970466	33.1361485005775	31.002582882408557	18.72511644104348
51	16.62655894089876	33.913494113398734	29.53104742267662	19.928899523025876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	911.0
1	1292.0
2	1673.0
3	21505.5
4	41338.0
5	28488.0
6	15638.0
7	14452.0
8	13266.0
9	14152.0
10	15038.0
11	16396.0
12	17754.0
13	18332.5
14	18911.0
15	18186.5
16	17462.0
17	16531.0
18	15600.0
19	14599.0
20	13598.0
21	13404.0
22	13210.0
23	13233.5
24	13257.0
25	13875.0
26	16110.5
27	17728.0
28	19720.0
29	21712.0
30	24524.5
31	27337.0
32	30485.5
33	33634.0
34	36529.0
35	39424.0
36	43069.5
37	46715.0
38	48741.5
39	50768.0
40	57218.0
41	63668.0
42	68749.0
43	73830.0
44	75692.0
45	77554.0
46	76946.5
47	76339.0
48	76624.0
49	76909.0
50	73543.0
51	70177.0
52	63653.0
53	57129.0
54	49457.5
55	41786.0
56	36885.5
57	31985.0
58	27929.5
59	23874.0
60	20596.5
61	17319.0
62	14564.0
63	11809.0
64	9461.5
65	7114.0
66	5607.0
67	4100.0
68	3049.5
69	1999.0
70	1718.0
71	1437.0
72	1122.5
73	808.0
74	638.5
75	371.5
76	274.0
77	197.0
78	120.0
79	86.0
80	52.0
81	40.5
82	29.0
83	32.5
84	36.0
85	23.0
86	10.0
87	13.0
88	16.0
89	10.5
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1088319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.33923672798448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.79765825719797	25.72613678394033
2	9.087304557602602	6.422768139608212
3	4.408016852711958	4.673278531767989
4	2.5681842075853734	3.630306786917229
5	1.6378631343375596	2.8940416516196823
6	1.170818429104029	2.4825497778956507
7	0.9088662281407999	2.248304715323665
8	0.7666957045530952	2.167555280122454
9	0.6449126413476053	2.0511648451307494
>10	5.881374353236321	40.52576832981033
>50	0.10993965614966325	2.4598851064354728
>100	0.015742266892748578	1.0173232833334462
>500	0.0015742266842017807	0.40073561998510926
>1k	7.871133421008904E-4	0.43893623901191736
>5k	0.0	0.0
>10k+	2.623711140336301E-4	2.8612449090977523
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30859	2.8354737903133183	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2473	0.22723117027268658	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT	1230	0.11301833377897472	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05724424548317175	0.0
2	0.0	0.0	0.0	0.1500479179358258	0.0
3	0.0	0.0	0.0	0.25681808366848324	0.0
4	0.0	0.0	0.0	0.37341992559166937	0.0
5	0.0	0.0	0.0	0.6963950826917475	0.0
6	0.0	0.0	0.0	1.2467851797129335	0.0
7	0.0	0.0	0.0	1.6504352124698733	0.0
8	0.0	0.0	0.0	2.259447827337389	0.0
9	0.0	0.0	0.0	2.6333271770501114	0.0
10	0.0	0.0	0.0	3.0457981529312637	0.0
11	0.0	0.0	0.0	3.4154508007303006	0.0
12	0.0	0.0	0.0	3.685684068733524	0.0
13	0.0	0.0	0.0	3.825257116709347	0.0
14	0.0	0.0	0.0	3.9070346102567353	0.0
15	0.0	0.0	0.0	3.9822882812851748	0.0
16	0.0	0.0	0.0	4.112580962015732	0.0
17	0.0	0.0	0.0	4.291572599577881	0.0
18	0.0	0.0	0.0	4.512004292858987	0.0
19	0.0	0.0	0.0	4.641286240523229	0.0
20	0.0	0.0	0.0	4.779756670608526	0.0
21	0.0	0.0	0.0	4.9429441184064595	0.0
22	0.0	0.0	0.0	5.129929735674926	0.0
23	0.0	0.0	0.0	5.3353842026097125	0.0
24	0.0	0.0	0.0	5.497836571813963	0.0
25	0.0	0.0	0.0	5.640074279691892	0.0
26	0.0	0.0	0.0	5.784517223350874	0.0
27	0.0	0.0	0.0	5.926387391931961	0.0
28	0.0	0.0	0.0	6.085991331585684	0.0
29	0.0	0.0	0.0	6.263237157487832	0.0
30	0.0	0.0	0.0	6.482658117702622	0.0
31	0.0	0.0	0.0	6.679567295985827	0.0
32	0.0	0.0	0.0	6.869768882101663	0.0
33	0.0	0.0	0.0	7.068240102396448	0.0
34	0.0	0.0	0.0	7.256971531324915	0.0
35	0.0	0.0	0.0	7.477586994254442	0.0
36	0.0	0.0	0.0	7.684419733552387	0.0
37	0.0	0.0	0.0	7.896398023006122	0.0
38	0.0	0.0	0.0	8.121423957497756	0.0
39	0.0	0.0	0.0	8.4118718868273	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCTA	20	7.0331217E-4	45.0	22
CGACGGT	90	0.0	45.0	28
CTCGGAC	20	7.0331217E-4	45.0	38
ACGCGAG	30	2.165396E-6	44.999996	1
CGTTTTT	12365	0.0	44.035583	1
CGGGATC	355	0.0	40.56338	6
ACGGGAT	650	0.0	40.499996	5
CGTTAGG	95	0.0	40.263157	2
TGGGCGA	415	0.0	40.12048	6
TTACGGG	310	0.0	39.919357	3
GGCGCGA	85	0.0	39.705883	8
GCGCGAC	80	0.0	39.375	9
CTAAGCG	40	3.4579898E-7	39.375	1
CGCGACC	80	0.0	39.375	10
CGTTCGG	40	3.4579898E-7	39.375	45
TCGGCGG	40	3.4579898E-7	39.375	2
GCGATCG	40	3.4579898E-7	39.375	9
GGCGATA	360	0.0	39.375	8
CGGTTTT	1070	0.0	39.32243	1
TACGGGA	460	0.0	39.130432	4
>>END_MODULE