FastQCFastQC Report
Thu 26 May 2016
SRR1547929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547929_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1767315
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231981.31261263555167No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20760.11746632603695437No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18340.10377323793438067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118500.043.158231
CGCGAGG2150.040.8139532
GCGCGAC950.040.2631579
CGACGGT2100.039.64285728
TACGGGA8600.039.2441864
AGGGCGA12900.039.2441836
CGCCGGT1450.038.79310228
TTAGGGA27950.038.640434
CGGTTTT9500.038.6052631
GGGCGAT24950.038.5971957
CGAGGGA14450.038.460214
TAAGGGA26750.038.271034
GCGATGA2650.038.2075469
TATGGGA24450.037.822094
CGGGATC6250.037.8000036
TAGGGTA8200.037.5914655
AGGGATT34250.037.510956
GTAATCG301.1401701E-437.5000041
CGTTTCG301.1401701E-437.50000442
ATAGGGA27050.037.2643284
Produced by FastQC (version 0.11.5)