##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547929_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1767315
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.428916746590165	31.0	31.0	33.0	30.0	34.0
2	31.804241462331277	31.0	31.0	34.0	30.0	34.0
3	31.729183535476132	31.0	31.0	34.0	30.0	34.0
4	35.628986343690855	37.0	35.0	37.0	33.0	37.0
5	35.63110877234675	37.0	35.0	37.0	33.0	37.0
6	35.71608230564444	37.0	35.0	37.0	33.0	37.0
7	35.98802250872085	37.0	35.0	37.0	35.0	37.0
8	36.008435394935255	37.0	35.0	37.0	35.0	37.0
9	37.79089522807196	39.0	38.0	39.0	35.0	39.0
10	37.30023849738162	39.0	37.0	39.0	34.0	39.0
11	37.10111100737559	39.0	37.0	39.0	33.0	39.0
12	36.773713231653666	39.0	35.0	39.0	33.0	39.0
13	36.685982408342596	39.0	35.0	39.0	33.0	39.0
14	37.816425481592134	40.0	36.0	41.0	33.0	41.0
15	37.945042621151295	40.0	36.0	41.0	33.0	41.0
16	37.990059497033634	40.0	36.0	41.0	33.0	41.0
17	37.9195565023779	40.0	36.0	41.0	33.0	41.0
18	37.756981070154445	39.0	36.0	41.0	33.0	41.0
19	37.64241971578355	39.0	36.0	41.0	33.0	41.0
20	37.42886582188235	39.0	35.0	41.0	33.0	41.0
21	37.315794298130214	39.0	35.0	41.0	32.0	41.0
22	37.257564158058976	39.0	35.0	41.0	32.0	41.0
23	37.09965059992135	39.0	35.0	41.0	32.0	41.0
24	37.0579342109358	39.0	35.0	41.0	32.0	41.0
25	37.082342423393676	39.0	35.0	41.0	32.0	41.0
26	36.99245861660202	39.0	35.0	41.0	32.0	41.0
27	36.92890118626278	39.0	35.0	41.0	32.0	41.0
28	36.862748293315	39.0	35.0	41.0	32.0	41.0
29	36.83697360119729	39.0	35.0	41.0	31.0	41.0
30	36.733340123294376	38.0	35.0	41.0	31.0	41.0
31	36.574831877735434	38.0	35.0	41.0	31.0	41.0
32	36.33636505093885	38.0	35.0	41.0	30.0	41.0
33	36.09823432721388	38.0	35.0	41.0	30.0	41.0
34	35.84151438764454	38.0	35.0	41.0	29.0	41.0
35	35.64120997105779	38.0	35.0	41.0	27.0	41.0
36	35.523070307217445	38.0	35.0	41.0	26.0	41.0
37	35.401471158225895	38.0	35.0	41.0	25.0	41.0
38	35.27586593221921	38.0	35.0	40.0	25.0	41.0
39	35.19907034116725	38.0	35.0	40.0	24.0	41.0
40	35.07962134650586	38.0	34.0	40.0	24.0	41.0
41	34.98165465692307	38.0	34.0	40.0	24.0	41.0
42	34.95578773450121	38.0	34.0	40.0	23.0	41.0
43	34.88336997083146	38.0	34.0	40.0	23.0	41.0
44	34.743018646930516	38.0	34.0	40.0	23.0	41.0
45	34.630948642432166	38.0	34.0	40.0	23.0	41.0
46	34.562625225271105	38.0	34.0	40.0	23.0	41.0
47	34.504518436158804	38.0	34.0	40.0	23.0	41.0
48	34.360150284471075	37.0	34.0	40.0	23.0	41.0
49	34.21501373552536	37.0	33.0	40.0	22.0	41.0
50	34.118795460911045	37.0	33.0	40.0	22.0	41.0
51	33.83214424140575	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	3.0
11	1.0
12	1.0
13	5.0
14	11.0
15	34.0
16	84.0
17	221.0
18	546.0
19	1106.0
20	2013.0
21	3466.0
22	5680.0
23	8891.0
24	14544.0
25	22945.0
26	30951.0
27	34241.0
28	34043.0
29	35030.0
30	39221.0
31	46596.0
32	57964.0
33	75078.0
34	119598.0
35	166700.0
36	139144.0
37	175593.0
38	277275.0
39	476256.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.628212853962083	24.20813493915912	28.50963184265397	16.654020364224827
2	33.92355069696121	25.326045441814276	28.527908154460295	12.222495706764215
3	27.590780364564328	24.626113624339744	34.877030976368104	12.90607503472782
4	24.274676557376583	26.89831750423665	34.55507365693156	14.271932281455202
5	22.41258632445263	31.292780291006412	32.69015427357319	13.604479110967768
6	20.66926382676546	39.98228951828055	28.95024373130993	10.398202923644059
7	85.66079052121438	3.1032385285022763	9.71066278507227	1.525308165211069
8	86.27624390671726	2.5447076497398595	9.482803009084401	1.6962454344584865
9	81.1181934176986	5.570766954391266	10.789474428723798	2.5215651991863366
10	34.37615818345909	38.53608439921576	16.870506955466343	10.217250461858809
11	28.793452214234588	24.940488820612057	30.08858070010157	16.177478265051786
12	27.685726653143327	22.513586994961283	31.470847019348557	18.32983933254683
13	22.750613218356662	27.408469910570552	32.2918664754161	17.54905039565669
14	17.87559093879699	31.149002865929393	32.519443336360524	18.455962858913097
15	17.227093076220143	27.705247791140795	37.90241128491525	17.16524784772381
16	20.718208129280857	26.49058034362861	35.827059692245015	16.964151834845513
17	20.53578450926971	25.64285370746019	31.495573794145354	22.325787989124745
18	21.125039961749888	26.285919601202956	34.145808755088936	18.443231681958224
19	23.00274710507182	27.308600900235668	30.77561159159516	18.91304040309735
20	24.263869202717117	27.640743161236113	30.587020423636986	17.508367212409787
21	22.636541872841004	27.11220127707851	32.90330246730209	17.347954382778397
22	22.367998913606232	24.300874490399277	32.20274823673199	21.1283783592625
23	19.00793010866767	28.66342446026883	31.866814914149433	20.461830516914077
24	18.714943289679542	27.86605670183301	34.72120137044047	18.69779863804698
25	19.36960870020342	28.823328042822023	32.78826921063874	19.018794046335827
26	19.18011220410623	30.475155815460177	30.64054794985614	19.70418403057746
27	18.680597403405734	29.98429821508899	32.367857456084515	18.967246925420767
28	17.71110413254004	28.77760897180186	34.74785196753267	18.763434928125434
29	18.53274600170315	27.00146832907546	34.70869652552035	19.757089143701037
30	20.012335095894056	27.2726141067099	33.69337101761712	19.02167977977893
31	22.40987034003559	27.58619714086057	30.96929523033528	19.034637288768554
32	22.921493904595387	27.09449079535906	31.555212285302847	18.428803014742705
33	22.579506200083177	27.64249723450545	30.359273813666494	19.41872275174488
34	20.5566636394757	27.538610830553694	31.88175282844315	20.022972701527458
35	20.261130585096602	27.873582241988554	31.491047153450292	20.374240019464555
36	23.56173064790374	27.787179987721487	29.63404939130828	19.01703997306649
37	21.1390159648959	29.510868181393807	30.41896888783267	18.931146965877616
38	20.615170470459425	30.230264553857122	29.225859566630735	19.928705409052718
39	20.87929995501651	29.01933158491837	29.776921488246295	20.324446971818833
40	22.022616228572723	27.646514628122322	29.84753708308932	20.483332060215638
41	19.74560279293731	27.649456944574112	30.225058917057794	22.37988134543078
42	20.765341775518227	28.133071919833192	29.084232295883872	22.01735400876471
43	20.923887365862907	27.87058334252807	30.114891799141635	21.090637492467387
44	20.793803028888455	29.03896588893321	29.454058840670733	20.713172241507596
45	19.884853577319266	30.411556513694503	28.187448191182668	21.51614171780356
46	21.494187510432493	29.036985483629117	28.87063143808546	20.59819556785293
47	20.523845494436475	28.812124607101737	29.929582445687387	20.734447452774408
48	20.898990842040043	27.862039308216136	30.82206624172827	20.41690360801555
49	21.312952133603797	26.987548908938138	30.462085140453173	21.237413817004892
50	20.040513434220838	28.85716468201764	30.39661859940079	20.705703284360737
51	19.475192594415823	28.94967790122304	29.263543850417157	22.31158565394398
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1094.0
1	1712.0
2	2330.0
3	18648.5
4	34967.0
5	25204.5
6	15442.0
7	14517.5
8	13593.0
9	14524.5
10	15456.0
11	16031.0
12	16606.0
13	16443.0
14	16280.0
15	15706.5
16	15133.0
17	13758.5
18	12384.0
19	12135.5
20	11887.0
21	11718.5
22	11550.0
23	12390.0
24	13230.0
25	14346.0
26	18964.0
27	22466.0
28	26543.0
29	30620.0
30	35122.5
31	39625.0
32	44736.0
33	49847.0
34	54934.5
35	60022.0
36	64930.0
37	69838.0
38	75152.5
39	80467.0
40	90531.0
41	100595.0
42	109621.0
43	118647.0
44	122784.0
45	126921.0
46	129576.5
47	132232.0
48	135333.5
49	138435.0
50	138182.5
51	137930.0
52	127693.0
53	117456.0
54	105365.5
55	93275.0
56	84047.5
57	74820.0
58	66387.0
59	57954.0
60	50367.5
61	42781.0
62	36532.5
63	30284.0
64	25217.0
65	20150.0
66	15562.0
67	10974.0
68	8801.5
69	6629.0
70	5305.0
71	3981.0
72	3243.0
73	2505.0
74	2087.5
75	1334.5
76	999.0
77	701.0
78	403.0
79	300.5
80	198.0
81	142.5
82	87.0
83	65.5
84	44.0
85	35.5
86	27.0
87	16.5
88	6.0
89	6.5
90	7.0
91	4.5
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1767315.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.97400005181026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.80988555235596	20.179218567651695
2	7.8651570377406905	4.243094926870264
3	3.0825480779783603	2.494459560452877
4	1.851576678193579	1.9977771765409706
5	1.2638165997039308	1.7045094512946248
6	0.9172412482771317	1.4844999287129916
7	0.716145008428096	1.3522106846110187
8	0.5834546390195599	1.2590484370514035
9	0.5162076629011408	1.2531766973256204
>10	7.683510185390227	49.281227544787704
>50	0.6707095229450836	11.098222686786709
>100	0.03698457520330687	1.6239443258786714
>500	0.0017004380694780555	0.3359229568853061
>1k	8.502190347390278E-4	0.3626408435397783
>5k	0.0	0.0
>10k+	2.1255475868475694E-4	1.3300462116103995
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23198	1.31261263555167	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2076	0.11746632603695437	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1834	0.10377323793438067	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06603237113927059	0.0
2	0.0	0.0	0.0	0.18610151557588772	0.0
3	0.0	0.0	0.0	0.31635560157640263	0.0
4	0.0	0.0	0.0	0.4587184514362182	0.0
5	0.0	0.0	0.0	0.8188693017373813	0.0
6	0.0	0.0	0.0	1.324042403306711	0.0
7	0.0	0.0	0.0	1.6486591241516084	0.0
8	0.0	0.0	0.0	2.253078822960253	0.0
9	0.0	0.0	0.0	2.6075713723925844	0.0
10	0.0	0.0	0.0	3.1283048013512023	0.0
11	0.0	0.0	0.0	3.6803286341144617	0.0
12	0.0	0.0	0.0	4.099212647434102	0.0
13	0.0	0.0	0.0	4.312756922223826	0.0
14	0.0	0.0	0.0	4.432486568608312	0.0
15	0.0	0.0	0.0	4.560986581339489	0.0
16	0.0	0.0	0.0	4.781547149206565	0.0
17	0.0	0.0	0.0	5.048562367206752	0.0
18	0.0	0.0	0.0	5.359372833931698	0.0
19	0.0	0.0	0.0	5.548869330028886	0.0
20	0.0	0.0	0.0	5.759244956332063	0.0
21	0.0	0.0	0.0	5.99927007918792	0.0
22	0.0	0.0	0.0	6.271321184961368	0.0
23	0.0	0.0	0.0	6.5591589501588565	0.0
24	0.0	0.0	0.0	6.7711754837139955	0.0
25	0.0	0.0	0.0	6.972497828627041	0.0
26	0.0	0.0	0.0	7.157807182081293	0.0
27	0.0	0.0	0.0	7.35064207569109	0.0
28	0.0	0.0	0.0	7.557170057403463	0.0
29	0.0	0.0	0.0	7.78270993003511	0.0
30	0.0	0.0	0.0	8.071113525319483	0.0
31	0.0	0.0	0.0	8.310629401097145	0.0
32	1.6974902606496295E-4	0.0	0.0	8.548278037588092	0.0
33	2.2633203475328392E-4	0.0	0.0	8.772007253941714	0.0
34	2.2633203475328392E-4	0.0	0.0	9.006091160885298	0.0
35	2.2633203475328392E-4	0.0	0.0	9.262751688295522	0.0
36	2.2633203475328392E-4	0.0	0.0	9.515111907045434	0.0
37	2.2633203475328392E-4	0.0	0.0	9.75887150847472	0.0
38	2.2633203475328392E-4	0.0	0.0	10.023227325066555	0.0
39	2.2633203475328392E-4	0.0	0.0	10.348579625024401	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11850	0.0	43.15823	1
CGCGAGG	215	0.0	40.813953	2
GCGCGAC	95	0.0	40.263157	9
CGACGGT	210	0.0	39.642857	28
TACGGGA	860	0.0	39.244186	4
AGGGCGA	1290	0.0	39.244183	6
CGCCGGT	145	0.0	38.793102	28
TTAGGGA	2795	0.0	38.64043	4
CGGTTTT	950	0.0	38.605263	1
GGGCGAT	2495	0.0	38.597195	7
CGAGGGA	1445	0.0	38.46021	4
TAAGGGA	2675	0.0	38.27103	4
GCGATGA	265	0.0	38.207546	9
TATGGGA	2445	0.0	37.82209	4
CGGGATC	625	0.0	37.800003	6
TAGGGTA	820	0.0	37.591465	5
AGGGATT	3425	0.0	37.51095	6
GTAATCG	30	1.1401701E-4	37.500004	1
CGTTTCG	30	1.1401701E-4	37.500004	42
ATAGGGA	2705	0.0	37.264328	4
>>END_MODULE