FastQCFastQC Report
Thu 26 May 2016
SRR1547928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547928_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3104939
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241640.7782439526187149No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37550.1209363533389867No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA207.035143E-445.026
ATACCCG207.035143E-445.033
GCGTACA253.892345E-545.09
CGGTCTA6950.043.70503631
CGTTTTT148850.043.050051
CGACGGT7100.042.1478928
GACGGTC7100.042.1478929
TACGGGA13150.042.0912554
ACGGGTA5300.042.02835
ACGGTCT7250.041.27586430
ACGGGAT12700.040.748035
TCACGAC7450.039.86577225
AGGGCGA26350.039.3643266
TCTCACG7700.039.1558423
GGGCGAT46450.039.090427
TAGGGTA24400.038.821725
TAGGGCG11200.038.7723245
CGTTAGG6400.038.6718752
CACGACG7650.038.5294126
CTACGGG7250.038.482763
Produced by FastQC (version 0.11.5)