##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547928_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3104939
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27705053142751	31.0	31.0	33.0	30.0	34.0
2	31.647680357005402	31.0	31.0	34.0	30.0	34.0
3	31.672322709077378	31.0	31.0	34.0	30.0	34.0
4	35.524548147322704	37.0	35.0	37.0	33.0	37.0
5	35.46272277812865	37.0	35.0	37.0	33.0	37.0
6	35.537315225838576	37.0	35.0	37.0	33.0	37.0
7	35.887642559161385	37.0	35.0	37.0	35.0	37.0
8	35.89829043340304	37.0	35.0	37.0	35.0	37.0
9	37.675252557296616	39.0	37.0	39.0	35.0	39.0
10	36.955834559068634	39.0	37.0	39.0	32.0	39.0
11	36.84818220261332	39.0	37.0	39.0	32.0	39.0
12	36.43142135803634	39.0	35.0	39.0	32.0	39.0
13	36.2809588207691	39.0	35.0	39.0	32.0	39.0
14	37.3533364101517	39.0	35.0	41.0	32.0	41.0
15	37.52305600850774	40.0	35.0	41.0	32.0	41.0
16	37.59559012270451	40.0	35.0	41.0	33.0	41.0
17	37.526355912306165	39.0	35.0	41.0	32.0	41.0
18	37.40460311780682	39.0	36.0	41.0	32.0	41.0
19	37.30343398050654	39.0	36.0	41.0	32.0	41.0
20	37.12368552168014	39.0	35.0	41.0	32.0	41.0
21	37.00846232405854	39.0	35.0	41.0	32.0	41.0
22	36.920007768268555	39.0	35.0	41.0	31.0	41.0
23	36.79232506661162	38.0	35.0	41.0	31.0	41.0
24	36.75195486932272	38.0	35.0	41.0	31.0	41.0
25	36.77415369512895	38.0	35.0	41.0	31.0	41.0
26	36.665021116356876	38.0	35.0	41.0	31.0	41.0
27	36.59096040212062	38.0	35.0	41.0	31.0	41.0
28	36.52578198798753	38.0	35.0	40.0	31.0	41.0
29	36.52114357158063	38.0	35.0	40.0	31.0	41.0
30	36.42439384477441	38.0	35.0	40.0	30.0	41.0
31	36.28258332933433	38.0	35.0	40.0	30.0	41.0
32	36.08471631809836	38.0	35.0	40.0	30.0	41.0
33	35.89538055336997	38.0	35.0	40.0	29.0	41.0
34	35.68177828936414	38.0	35.0	40.0	27.0	41.0
35	35.551186351809164	38.0	35.0	40.0	27.0	41.0
36	35.43038752130074	38.0	35.0	40.0	26.0	41.0
37	35.32972499620765	38.0	34.0	40.0	26.0	41.0
38	35.227675970445794	38.0	34.0	40.0	25.0	41.0
39	35.168786568753845	38.0	34.0	40.0	25.0	41.0
40	35.02878993758009	38.0	34.0	40.0	24.0	41.0
41	34.93630019784608	38.0	34.0	40.0	24.0	41.0
42	34.913638883082726	38.0	34.0	40.0	24.0	41.0
43	34.84692774962729	38.0	34.0	40.0	24.0	41.0
44	34.67871317278697	38.0	34.0	40.0	23.0	41.0
45	34.562047756815836	38.0	34.0	40.0	23.0	41.0
46	34.507627364015846	37.0	34.0	40.0	23.0	41.0
47	34.45355705860888	37.0	33.0	40.0	23.0	41.0
48	34.33455150004557	37.0	33.0	40.0	23.0	41.0
49	34.20002872842269	37.0	33.0	40.0	23.0	41.0
50	34.112322013411536	37.0	33.0	40.0	23.0	41.0
51	33.790725679312864	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	7.0
13	15.0
14	26.0
15	98.0
16	208.0
17	543.0
18	1259.0
19	2585.0
20	4562.0
21	7510.0
22	11622.0
23	17957.0
24	27259.0
25	40861.0
26	53724.0
27	59267.0
28	61661.0
29	66699.0
30	76956.0
31	92159.0
32	112369.0
33	143752.0
34	228160.0
35	315911.0
36	229413.0
37	296668.0
38	461548.0
39	792006.0
40	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.0050087940536	24.53172187923821	28.999571328132372	14.463697998575817
2	31.733022774360464	25.382302196597102	29.576201013932963	13.308474015109475
3	27.995525838027735	24.737072129275326	33.513283191714876	13.754118840982061
4	24.503991865862744	27.43934743967595	33.59550702928463	14.461153665176674
5	22.37254902592289	32.2125491032191	31.617851429609406	13.797050441248604
6	20.67093749667868	40.4997006382412	28.28248799734874	10.54687386773138
7	87.53144586737453	2.9451786331390086	8.06627763057503	1.4570978689114344
8	88.12070059991517	2.4656523042803737	7.589102394604209	1.8245447012002491
9	83.18537014736843	4.984896643702179	9.449396590400005	2.3803366185293817
10	33.09266301205918	32.51674187480012	23.03397908944427	11.35661602369644
11	32.77797083936271	23.982500139294203	26.860237833979987	16.3792911873631
12	30.23273565116738	22.580604643118594	29.635525850910437	17.551133854803588
13	23.032272131594212	30.19376548138305	29.941425580341512	16.832536806681226
14	18.5690604549719	32.29403218549543	31.190145764538368	17.9467615949943
15	17.264300522490135	27.189455251777893	38.69380364638403	16.852440579347935
16	20.263071190770575	24.847025980220547	36.87821242220862	18.011690406800263
17	20.013629897398953	25.557635753874713	29.762613693860008	24.666120654866326
18	21.21668090741879	25.853454769964884	33.834770989059685	19.095093333556633
19	24.026333528613605	28.235949240870756	28.550126105537014	19.187591124978624
20	24.892534120638118	27.194157437553525	29.43133504394128	18.481973397867076
21	21.99730815967721	28.032434775691247	31.36615566360563	18.604101401025915
22	21.86110580594337	24.450045556450544	30.735450841385294	22.953397796220795
23	18.871063167424545	27.961483301282247	31.006985966551998	22.160467564741207
24	19.050100501169265	26.60152099606466	35.8674679277113	18.480910575054775
25	18.603199611973054	29.141538690454144	32.22253319630434	20.032728501268462
26	18.738822244172912	31.711283216836144	29.150331133719536	20.399563405271408
27	18.14354484902924	31.05130245714972	31.399006550531265	19.40614614328977
28	17.27898680135101	28.866203168564663	34.70444991028809	19.150360119796233
29	17.60153742150812	26.402257822134345	34.16160510721789	21.834599649139644
30	19.210973226849223	29.270913212787754	32.161404781221144	19.356708779141876
31	22.671137822675423	28.40013926199516	29.025948657928545	19.90277425740087
32	23.51556665042373	27.997941344419324	29.072906102181072	19.41358590297587
33	22.937197800021192	28.433827524469883	28.016363606499194	20.612611069009727
34	18.72439361932714	28.759437786056345	30.125487167380744	22.390681427235766
35	19.429624865416034	28.165770728507066	30.237566663950567	22.167037742126336
36	23.28512734066595	28.088410110472378	28.82832802834452	19.79813452051715
37	19.540158437895236	30.507974552801198	29.812824020053213	20.139042989250353
38	19.485020478663188	30.78479158527752	28.472346799727788	21.257841136331503
39	19.23425870846416	30.248967854118874	28.67093363186845	21.845839805548515
40	20.855353357988676	27.984607749137748	28.87132404211484	22.288714850758744
41	18.043832745184364	26.786774232923737	29.892857798494592	25.276535223397307
42	20.301526052524704	27.81974138622369	27.934622870207754	23.94410969104385
43	20.16081475352656	28.3649694889336	28.921405541300487	22.55281021623935
44	19.62817949080481	29.896658195217363	28.381105071629424	22.0940572423484
45	18.207893939301222	32.069325677573694	26.71533965723642	23.007440725888657
46	20.551450447174645	30.302817543275406	27.87562010074916	21.270111908800786
47	19.799036309570013	28.572123317076436	29.26511599744794	22.363724375905612
48	20.198528859987267	27.572296911469113	30.45625050926926	21.772923719274356
49	20.231798434687445	26.659621976470394	30.201817169355017	22.906762419487144
50	19.28665909378574	29.32550365723771	29.595267411050585	21.792569837925964
51	18.24663866182234	29.71942443957836	28.457338453348036	23.57659844525126
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2250.0
1	3039.5
2	3829.0
3	21016.0
4	38203.0
5	28900.5
6	19598.0
7	18635.0
8	17672.0
9	18342.0
10	19012.0
11	19507.5
12	20003.0
13	19976.0
14	19949.0
15	19102.0
16	18255.0
17	17648.0
18	17041.0
19	16841.0
20	16641.0
21	17299.0
22	17957.0
23	20104.0
24	22251.0
25	24446.0
26	30804.0
27	34967.0
28	42948.0
29	50929.0
30	62111.0
31	73293.0
32	81056.0
33	88819.0
34	100048.5
35	111278.0
36	121829.0
37	132380.0
38	143099.5
39	153819.0
40	174765.0
41	195711.0
42	217726.5
43	239742.0
44	243104.5
45	246467.0
46	244902.5
47	243338.0
48	248821.5
49	254305.0
50	249736.5
51	245168.0
52	230977.5
53	216787.0
54	188135.0
55	159483.0
56	140914.5
57	122346.0
58	104545.0
59	86744.0
60	75535.5
61	64327.0
62	55811.5
63	47296.0
64	38705.5
65	30115.0
66	24188.0
67	18261.0
68	14796.5
69	11332.0
70	9456.5
71	7581.0
72	6242.0
73	4903.0
74	3890.5
75	2138.5
76	1399.0
77	1159.5
78	920.0
79	732.5
80	545.0
81	390.0
82	235.0
83	171.5
84	108.0
85	91.0
86	74.0
87	63.0
88	52.0
89	35.0
90	18.0
91	12.5
92	7.0
93	8.0
94	9.0
95	5.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3104939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.684666449893893
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.20426160088971	18.28557184527424
2	9.259028694651047	4.385940125656131
3	3.287552284573044	2.335937378900976
4	1.7205895597992447	1.630063592840595
5	1.0317221330110973	1.2217997294670453
6	0.6878988711256956	0.9775593188322388
7	0.5028863250810559	0.8337486410230404
8	0.4120123369593717	0.7806699819299209
9	0.3203468620507491	0.6828577718347947
>10	3.401958257729118	18.616166673699027
>50	1.3991758763325786	24.218672415189857
>100	0.7672878365517055	24.048636370525088
>500	0.0037511250956172295	0.6170719567013379
>1k	0.001389305590969344	0.5701819903866465
>5k	0.0	0.0
>10k+	1.389305590969344E-4	0.7951222077390886
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24164	0.7782439526187149	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3755	0.1209363533389867	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.220675188787928E-5	0.0	0.0	0.062255651399270646	0.0
2	3.220675188787928E-5	0.0	0.0	0.19220989526686352	0.0
3	3.220675188787928E-5	0.0	0.0	0.30628621045373194	0.0
4	3.220675188787928E-5	0.0	0.0	0.5035525657669925	0.0
5	3.220675188787928E-5	0.0	0.0	0.896120664528353	0.0
6	3.220675188787928E-5	0.0	0.0	1.4348107966050219	0.0
7	3.220675188787928E-5	0.0	0.0	1.7481502857221993	0.0
8	3.220675188787928E-5	0.0	0.0	2.4404344175521646	0.0
9	3.220675188787928E-5	0.0	0.0	2.7129035385236233	0.0
10	3.220675188787928E-5	0.0	0.0	3.204571812843988	0.0
11	3.220675188787928E-5	0.0	0.0	3.74200588159703	0.0
12	3.220675188787928E-5	0.0	0.0	4.149292465971151	0.0
13	3.220675188787928E-5	0.0	0.0	4.345882479494766	0.0
14	3.220675188787928E-5	0.0	0.0	4.43467649444965	0.0
15	3.220675188787928E-5	0.0	0.0	4.558382628450993	0.0
16	3.220675188787928E-5	0.0	0.0	4.836552344506607	0.0
17	3.220675188787928E-5	0.0	0.0	5.176301370171846	0.0
18	3.220675188787928E-5	0.0	0.0	5.587162904005521	0.0
19	3.220675188787928E-5	0.0	0.0	5.81286782123578	0.0
20	3.220675188787928E-5	0.0	0.0	6.055642316966614	0.0
21	3.220675188787928E-5	0.0	0.0	6.390882397367549	0.0
22	3.220675188787928E-5	0.0	0.0	6.748087482556018	0.0
23	3.220675188787928E-5	0.0	0.0	7.162813826616239	0.0
24	3.220675188787928E-5	0.0	0.0	7.455090099998744	0.0
25	3.220675188787928E-5	0.0	0.0	7.727527014218315	0.0
26	3.220675188787928E-5	0.0	0.0	7.981573873109906	0.0
27	3.220675188787928E-5	0.0	0.0	8.246474407387714	0.0
28	3.220675188787928E-5	0.0	0.0	8.525320465232973	0.0
29	3.220675188787928E-5	0.0	0.0	8.843619794140883	0.0
30	3.220675188787928E-5	0.0	0.0	9.203787900503038	0.0
31	3.220675188787928E-5	0.0	0.0	9.534100347865127	0.0
32	3.220675188787928E-5	0.0	0.0	9.839130494995231	0.0
33	3.220675188787928E-5	0.0	0.0	10.146704975524479	0.0
34	3.220675188787928E-5	0.0	0.0	10.462524384537023	0.0
35	3.220675188787928E-5	0.0	0.0	10.823336625936935	0.0
36	3.220675188787928E-5	0.0	0.0	11.155484858156633	0.0
37	3.220675188787928E-5	0.0	0.0	11.508825133118558	0.0
38	3.220675188787928E-5	0.0	0.0	11.86200437432104	0.0
39	3.220675188787928E-5	0.0	0.0	12.286296123691963	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTA	20	7.035143E-4	45.0	26
ATACCCG	20	7.035143E-4	45.0	33
GCGTACA	25	3.892345E-5	45.0	9
CGGTCTA	695	0.0	43.705036	31
CGTTTTT	14885	0.0	43.05005	1
CGACGGT	710	0.0	42.14789	28
GACGGTC	710	0.0	42.14789	29
TACGGGA	1315	0.0	42.091255	4
ACGGGTA	530	0.0	42.0283	5
ACGGTCT	725	0.0	41.275864	30
ACGGGAT	1270	0.0	40.74803	5
TCACGAC	745	0.0	39.865772	25
AGGGCGA	2635	0.0	39.364326	6
TCTCACG	770	0.0	39.15584	23
GGGCGAT	4645	0.0	39.09042	7
TAGGGTA	2440	0.0	38.82172	5
TAGGGCG	1120	0.0	38.772324	5
CGTTAGG	640	0.0	38.671875	2
CACGACG	765	0.0	38.52941	26
CTACGGG	725	0.0	38.48276	3
>>END_MODULE