FastQCFastQC Report
Thu 26 May 2016
SRR1547927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547927_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2257419
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT515862.2851761237058783No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41400.18339528461486326No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT37860.16771365882895464No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC31480.13945129371197815No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT27550.12204203118694402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT221350.043.271971
ACGGGAT11150.040.560545
CGACGGT3150.039.99999628
CGGTCTA3150.039.99999631
CGGTTTT18900.039.4047621
TAGTACG750.039.01
CGGTAGT4600.038.64130812
TACGGGA10350.038.478264
AGGGCGA16000.038.3906256
TAGGGAT37850.038.223255
TAAGGGA33300.038.108114
CTCACGA3250.038.07692324
GGGCGAT29650.038.0185477
ATAGGGA37700.038.017244
AGGGATC26550.037.8813556
CGAGGGA17100.037.631584
TAGGGAC25950.037.543355
TCGTTAG601.5643309E-1037.5000041
CACGCCG4800.037.50000426
TCGTAAG1200.037.5000041