##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547927_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2257419
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.352114516622745	31.0	31.0	33.0	30.0	34.0
2	31.692362826750372	31.0	31.0	34.0	30.0	34.0
3	31.470264935308865	31.0	31.0	34.0	30.0	34.0
4	35.44910625807615	37.0	35.0	37.0	33.0	37.0
5	35.483552676751636	37.0	35.0	37.0	33.0	37.0
6	35.58517758555235	37.0	35.0	37.0	33.0	37.0
7	35.920318292705076	37.0	35.0	37.0	35.0	37.0
8	35.94330250609213	37.0	35.0	37.0	35.0	37.0
9	37.71331817442841	39.0	37.0	39.0	35.0	39.0
10	37.23049952179901	39.0	37.0	39.0	34.0	39.0
11	37.033822254530506	39.0	37.0	39.0	33.0	39.0
12	36.663078941038414	39.0	35.0	39.0	32.0	39.0
13	36.5464067592237	39.0	35.0	39.0	32.0	39.0
14	37.520366400743505	40.0	36.0	41.0	32.0	41.0
15	37.69951302793146	40.0	36.0	41.0	32.0	41.0
16	37.782063941164665	40.0	36.0	41.0	33.0	41.0
17	37.738653745715794	40.0	36.0	41.0	33.0	41.0
18	37.543883966600795	39.0	36.0	41.0	32.0	41.0
19	37.367415176358485	39.0	36.0	41.0	32.0	41.0
20	37.07910095556031	39.0	35.0	41.0	32.0	41.0
21	36.96999980951698	39.0	35.0	41.0	32.0	41.0
22	36.95046954065683	38.0	35.0	41.0	32.0	41.0
23	36.81933703933563	38.0	35.0	41.0	32.0	41.0
24	36.76792079804414	38.0	35.0	41.0	32.0	41.0
25	36.75257451097913	38.0	35.0	41.0	31.0	41.0
26	36.66902068246967	38.0	35.0	41.0	31.0	41.0
27	36.60311887159628	38.0	35.0	41.0	31.0	41.0
28	36.513323401637	38.0	35.0	40.0	31.0	41.0
29	36.45787024916508	38.0	35.0	40.0	31.0	41.0
30	36.31039474727554	38.0	35.0	40.0	30.0	41.0
31	36.05012538655872	38.0	35.0	40.0	30.0	41.0
32	35.77336728361018	38.0	35.0	40.0	29.0	41.0
33	35.4224944505207	38.0	34.0	40.0	26.0	41.0
34	35.06214708036036	38.0	34.0	40.0	24.0	41.0
35	34.805275848214265	38.0	34.0	40.0	23.0	41.0
36	34.65942432485949	38.0	34.0	40.0	22.0	41.0
37	34.53000306987759	38.0	34.0	40.0	21.0	41.0
38	34.36939930070581	38.0	34.0	40.0	20.0	41.0
39	34.256739222979874	38.0	33.0	40.0	19.0	41.0
40	34.12684619027305	38.0	33.0	40.0	18.0	41.0
41	33.974345037407765	38.0	33.0	40.0	18.0	41.0
42	33.963172100527196	38.0	33.0	40.0	18.0	41.0
43	33.91357563660092	38.0	33.0	40.0	18.0	41.0
44	33.783654695916	37.0	33.0	40.0	18.0	41.0
45	33.68578717553099	37.0	33.0	40.0	18.0	41.0
46	33.65073696996437	37.0	33.0	40.0	18.0	41.0
47	33.579805964245004	37.0	33.0	40.0	18.0	41.0
48	33.432225918183555	37.0	33.0	40.0	18.0	41.0
49	33.29950797791637	36.0	32.0	40.0	18.0	41.0
50	33.219354492896535	36.0	32.0	40.0	18.0	41.0
51	32.95334140449779	36.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	4.0
13	10.0
14	24.0
15	90.0
16	220.0
17	582.0
18	1221.0
19	2390.0
20	4245.0
21	6623.0
22	10328.0
23	15585.0
24	24358.0
25	38467.0
26	53116.0
27	57968.0
28	55773.0
29	55236.0
30	58504.0
31	67317.0
32	79538.0
33	100125.0
34	154172.0
35	198601.0
36	165079.0
37	212511.0
38	330508.0
39	564745.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.077491152506468	22.65086809316303	26.935274311060553	20.336366443269945
2	36.96730646813906	23.569837943244032	27.31460132124342	12.148254267373492
3	27.059708454655517	23.502681602307767	37.257992424091405	12.179617518945308
4	23.76514949152107	25.534205214007677	37.134975828590086	13.565669465881166
5	21.816729636810887	29.656036384915694	35.73926683526629	12.787967143007126
6	20.493847176797928	37.47173209758578	32.23513224616254	9.799288479453748
7	81.88834239456654	2.6958663854605636	13.685762368439356	1.7300288515335434
8	82.36982146424744	2.192503917084068	13.453993255128976	1.9836813635395114
9	77.23169690695435	4.868391734099872	15.097773164840023	2.8021381941057464
10	32.38446207815208	36.07832661991416	20.62966600351995	10.907545298413808
11	28.190690341491763	23.179037653178252	33.2645379524138	15.365734052916185
12	27.77849393488759	20.92828136912111	34.39618431491894	16.89704038107237
13	23.66432638336082	23.21748864521828	35.92961696521558	17.18856800620532
14	17.493739531739568	26.30703471530983	38.13160073517588	18.067625017774724
15	16.812386180855217	25.331185747971467	41.578546118376785	16.277881952796534
16	22.18095090012089	24.485706906870192	37.78058038848792	15.552761804521003
17	21.662526983249457	24.231257024061552	34.3561828796515	19.7500331130375
18	21.32395448075878	24.835176810330736	36.39829380367579	17.442574905234697
19	22.842768666339744	25.744046630244537	33.73449944383387	17.67868525958185
20	22.47642108088928	27.521784834804702	33.023510478116826	16.978283606189194
21	21.781246636091925	25.230849921968407	36.40059731932796	16.587306122611707
22	20.898867246178046	22.488558836441086	36.920084397269626	19.69248952011124
23	18.306083186151973	25.45996113260321	36.738549644527666	19.495406036717153
24	18.52008864991391	26.098345056899053	37.236994992954344	18.1445713002327
25	20.800967830960936	26.108489385444173	34.595527015587265	18.495015768007622
26	18.64682630916104	28.043974113799873	32.978326132632	20.330873444407086
27	18.218593889747538	26.743506633017617	36.40086310959552	18.637036367639325
28	17.38702473931512	26.524052468770755	36.67263365817334	19.41628913374079
29	19.153378260748227	26.028132127885872	35.58776638275836	19.230723228607538
30	21.15938600676259	25.171091410145834	35.59108876110283	18.07843382198874
31	22.163010057060742	24.92479242887563	34.75190028966709	18.160297224396533
32	24.425505411268354	25.125198290614193	32.945279542698984	17.504016755418466
33	23.193124537358816	25.357144597436275	33.40686864069099	18.042862224513925
34	20.420754853219538	26.602061912298957	34.7648797143995	18.212303520082003
35	20.137511024758805	27.90403553793071	33.42055683947021	18.537896597840277
36	23.81414349750755	27.420828831510676	31.27753421052981	17.487493460451958
37	20.876895250726605	30.33544060717129	31.23496346934264	17.552700672759467
38	21.74354871647665	29.11785539148913	30.385364879094222	18.75323101294
39	20.36764109808591	28.749824467677463	30.50838147459555	20.374152959641076
40	22.58114244630704	27.203456691026346	30.96142098564777	19.253979877018843
41	19.483268281165348	27.286560448016072	31.23230556666707	21.99786570415151
42	20.030574740444727	27.94120187701087	31.449190424994207	20.579032957550194
43	20.023708491866156	27.500211524754597	31.915873836447734	20.560206146931517
44	21.01306846447204	28.024615722646086	30.999473292286456	19.962842520595423
45	19.535673262252157	28.584237130988978	30.054588891118573	21.825500715640295
46	20.632811188352715	27.959098421693092	30.643048543491485	20.765041846462708
47	19.780643292184568	27.822039240389135	32.152648666463776	20.244668800962515
48	19.961292077368004	27.960781760054292	31.90608389492602	20.171842267651684
49	20.86670662380356	26.51842657477411	31.676219611866475	20.93864718955586
50	18.860300192387854	27.91138906866647	32.56635121791746	20.66195952102822
51	18.680138689361613	28.46480870409968	31.537477092201314	21.317575514337392
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1671.0
1	2986.5
2	4302.0
3	40942.0
4	77582.0
5	54006.0
6	30430.0
7	26960.0
8	23490.0
9	23757.5
10	24025.0
11	24065.5
12	24106.0
13	23737.0
14	23368.0
15	22037.5
16	20707.0
17	19812.0
18	18917.0
19	18329.5
20	17742.0
21	18669.0
22	19596.0
23	18969.0
24	18342.0
25	20806.5
26	26894.5
27	30518.0
28	33764.0
29	37010.0
30	43184.0
31	49358.0
32	54891.0
33	60424.0
34	67261.5
35	74099.0
36	77600.0
37	81101.0
38	87366.0
39	93631.0
40	102967.0
41	112303.0
42	122912.5
43	133522.0
44	139305.0
45	145088.0
46	157997.0
47	170906.0
48	173507.5
49	176109.0
50	170190.0
51	164271.0
52	148057.5
53	131844.0
54	121684.0
55	111524.0
56	103773.0
57	96022.0
58	87569.0
59	79116.0
60	69755.5
61	60395.0
62	51682.0
63	42969.0
64	36793.5
65	30618.0
66	25033.5
67	19449.0
68	15784.0
69	12119.0
70	9754.5
71	7390.0
72	5961.0
73	4532.0
74	3559.5
75	1930.0
76	1273.0
77	1062.0
78	851.0
79	610.0
80	369.0
81	316.0
82	263.0
83	192.5
84	122.0
85	80.5
86	39.0
87	28.5
88	18.0
89	11.0
90	4.0
91	9.0
92	14.0
93	9.5
94	5.0
95	6.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2257419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.373033092965706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.1807252407435	20.031784137821024
2	7.492394737847372	4.101791382093164
3	3.2743761617021576	2.6888882109927352
4	2.1070861767453333	2.3070935858312236
5	1.4522136910889938	1.9875746712118452
6	1.1103795293173562	1.8236673361053402
7	0.9130844012218019	1.7495722671920608
8	0.777964774724453	1.7036204418955245
9	0.7010009748696574	1.7269670594977515
>10	8.633792645418042	51.20242883774957
>50	0.32032822692802904	5.3333462348040355
>100	0.032380350480363085	1.5208160521686054
>500	0.0023008940300985876	0.44164192587885537
>1k	0.001807845309363176	1.060084581991377
>5k	0.0	0.0
>10k+	1.6434957357847055E-4	2.3207232747668973
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51586	2.2851761237058783	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4140	0.18339528461486326	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT	3786	0.16771365882895464	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	3148	0.13945129371197815	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT	2755	0.12204203118694402	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06343527719045512	0.0
2	0.0	0.0	0.0	0.178256672775413	0.0
3	0.0	0.0	0.0	0.34003434896224405	0.0
4	0.0	0.0	0.0	0.4899400598648279	0.0
5	0.0	0.0	0.0	0.9123250933920553	0.0
6	0.0	0.0	0.0	1.6491843118180542	0.0
7	0.0	0.0	0.0	2.163975761699534	0.0
8	0.0	0.0	0.0	3.0041387974496536	0.0
9	0.0	0.0	0.0	3.575853662966423	0.0
10	0.0	0.0	0.0	4.177691425473073	0.0
11	0.0	0.0	0.0	4.62519363928451	0.0
12	0.0	0.0	0.0	4.925802431892351	0.0
13	0.0	0.0	0.0	5.095553816105916	0.0
14	0.0	0.0	0.0	5.2593692176773565	0.0
15	0.0	0.0	0.0	5.352573004834282	0.0
16	0.0	0.0	0.0	5.470805375519564	0.0
17	4.429837792629547E-5	0.0	0.0	5.618850554549244	0.0
18	4.429837792629547E-5	0.0	0.0	5.800828291070466	0.0
19	4.429837792629547E-5	0.0	0.0	5.9240220800834935	0.0
20	4.429837792629547E-5	0.0	0.0	6.062233019213536	0.0
21	4.429837792629547E-5	0.0	0.0	6.2180304143803165	0.0
22	4.429837792629547E-5	0.0	0.0	6.400451134680801	0.0
23	4.429837792629547E-5	0.0	0.0	6.586061338191979	0.0
24	4.429837792629547E-5	0.0	0.0	6.732733267505944	0.0
25	4.429837792629547E-5	0.0	0.0	6.866425772087504	0.0
26	4.429837792629547E-5	0.0	0.0	7.004016533926578	0.0
27	4.429837792629547E-5	0.0	0.0	7.174919675966225	0.0
28	4.429837792629547E-5	0.0	0.0	7.3339065543437	0.0
29	4.429837792629547E-5	0.0	0.0	7.512163227119113	0.0
30	4.429837792629547E-5	0.0	0.0	7.757266152185306	0.0
31	4.429837792629547E-5	0.0	0.0	7.954969812870362	0.0
32	4.429837792629547E-5	0.0	0.0	8.160337092936668	0.0
33	4.429837792629547E-5	0.0	0.0	8.347010457518078	0.0
34	4.429837792629547E-5	0.0	0.0	8.562256275861946	0.0
35	4.429837792629547E-5	0.0	0.0	8.792696437834536	0.0
36	4.429837792629547E-5	0.0	0.0	8.999924249773747	0.0
37	4.429837792629547E-5	0.0	0.0	9.229478444187809	0.0
38	4.429837792629547E-5	0.0	0.0	9.506387604605083	0.0
39	4.429837792629547E-5	0.0	0.0	10.051036161208884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	22135	0.0	43.27197	1
ACGGGAT	1115	0.0	40.56054	5
CGACGGT	315	0.0	39.999996	28
CGGTCTA	315	0.0	39.999996	31
CGGTTTT	1890	0.0	39.404762	1
TAGTACG	75	0.0	39.0	1
CGGTAGT	460	0.0	38.641308	12
TACGGGA	1035	0.0	38.47826	4
AGGGCGA	1600	0.0	38.390625	6
TAGGGAT	3785	0.0	38.22325	5
TAAGGGA	3330	0.0	38.10811	4
CTCACGA	325	0.0	38.076923	24
GGGCGAT	2965	0.0	38.018547	7
ATAGGGA	3770	0.0	38.01724	4
AGGGATC	2655	0.0	37.881355	6
CGAGGGA	1710	0.0	37.63158	4
TAGGGAC	2595	0.0	37.54335	5
TCGTTAG	60	1.5643309E-10	37.500004	1
CACGCCG	480	0.0	37.500004	26
TCGTAAG	120	0.0	37.500004	1
>>END_MODULE