FastQCFastQC Report
Thu 26 May 2016
SRR1547926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547926_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2668847
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365391.3690930952579898No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT32960.12349902411041172No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30230.1132698877080627No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC27740.1039400160443817No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27510.10307822066982485No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA406.8212103E-945.00000414
CGGTCTA4100.045.00000431
TATCCGC207.0349645E-445.00000420
CGTTTTT159100.043.5009421
CGACGGT4400.042.4431828
GCGCGAC3050.040.5737729
TAGGGTA19350.039.767445
TAACGGG6400.039.3750043
TAGGGCG8350.039.3413165
TAGTGCG1450.038.7931061
ACGGGAT9950.038.442215
ATTAGCG1650.038.1818161
GGGCGAT37850.038.104367
TACGTTG659.094947E-1238.0769271
TAAGGGT16600.037.6807254
TGGGCGA10900.037.568816
TAGCGAT301.1403263E-437.50000428
CGCGATT301.1403263E-437.50000411
CGAGGGT8100.037.54
CGGTTTT14050.037.1530271