##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547926_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2668847
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31810440988187	31.0	31.0	33.0	30.0	34.0
2	31.68631510161504	31.0	31.0	34.0	30.0	34.0
3	31.652809246839553	31.0	31.0	34.0	30.0	34.0
4	35.534332241600964	37.0	35.0	37.0	33.0	37.0
5	35.51433671544304	37.0	35.0	37.0	33.0	37.0
6	35.583615696216384	37.0	35.0	37.0	33.0	37.0
7	35.9071580349117	37.0	35.0	37.0	35.0	37.0
8	35.914368639341255	37.0	35.0	37.0	35.0	37.0
9	37.66691496365284	39.0	37.0	39.0	35.0	39.0
10	37.043560009247436	39.0	37.0	39.0	33.0	39.0
11	36.933626393719834	39.0	37.0	39.0	33.0	39.0
12	36.52462018242335	39.0	35.0	39.0	32.0	39.0
13	36.412339111234175	39.0	35.0	39.0	32.0	39.0
14	37.46768923059284	40.0	36.0	41.0	32.0	41.0
15	37.61861807739447	40.0	36.0	41.0	32.0	41.0
16	37.68488751884241	40.0	36.0	41.0	33.0	41.0
17	37.62018017518427	40.0	36.0	41.0	33.0	41.0
18	37.478501015607115	39.0	36.0	41.0	32.0	41.0
19	37.367729959791625	39.0	36.0	41.0	32.0	41.0
20	37.177674104210546	39.0	35.0	41.0	32.0	41.0
21	37.06753628064853	39.0	35.0	41.0	32.0	41.0
22	36.98176815681079	39.0	35.0	41.0	32.0	41.0
23	36.84569553818559	38.0	35.0	41.0	31.0	41.0
24	36.79136083859434	38.0	35.0	41.0	31.0	41.0
25	36.81446369911801	38.0	35.0	41.0	31.0	41.0
26	36.7082020812733	38.0	35.0	41.0	31.0	41.0
27	36.63651494446853	38.0	35.0	41.0	31.0	41.0
28	36.56402596327178	38.0	35.0	40.0	31.0	41.0
29	36.53568563503266	38.0	35.0	40.0	31.0	41.0
30	36.435651800196865	38.0	35.0	40.0	31.0	41.0
31	36.267717482493374	38.0	35.0	40.0	30.0	41.0
32	36.06045269736332	38.0	35.0	40.0	30.0	41.0
33	35.850156640676666	38.0	35.0	40.0	29.0	41.0
34	35.60191460956735	38.0	35.0	40.0	27.0	41.0
35	35.43570650546847	38.0	35.0	40.0	27.0	41.0
36	35.331272268511455	38.0	35.0	40.0	26.0	41.0
37	35.21940485910208	38.0	34.0	40.0	25.0	41.0
38	35.10224827425476	38.0	34.0	40.0	25.0	41.0
39	35.018932520298094	38.0	34.0	40.0	24.0	41.0
40	34.88816518893739	38.0	34.0	40.0	24.0	41.0
41	34.77621159999056	38.0	34.0	40.0	23.0	41.0
42	34.774847715136914	38.0	34.0	40.0	23.0	41.0
43	34.72226133607509	38.0	34.0	40.0	23.0	41.0
44	34.58950175862461	38.0	34.0	40.0	23.0	41.0
45	34.47459408501124	37.0	34.0	40.0	23.0	41.0
46	34.415776550697736	37.0	34.0	40.0	23.0	41.0
47	34.342861168137404	37.0	33.0	40.0	23.0	41.0
48	34.193731225506745	37.0	33.0	40.0	23.0	41.0
49	34.049334787644256	37.0	33.0	40.0	22.0	41.0
50	33.94454796397096	36.0	33.0	40.0	22.0	41.0
51	33.63047038665012	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	2.0
12	6.0
13	10.0
14	33.0
15	72.0
16	185.0
17	478.0
18	1151.0
19	2256.0
20	3780.0
21	6469.0
22	10110.0
23	15317.0
24	23466.0
25	35029.0
26	47017.0
27	51642.0
28	53478.0
29	57751.0
30	65335.0
31	77955.0
32	95466.0
33	122733.0
34	194681.0
35	269286.0
36	212363.0
37	260062.0
38	397866.0
39	664734.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.05522834392529	24.035697812575993	27.88425113916234	16.024822704336366
2	34.37671773616097	24.986070763891675	27.946862446592107	12.690349053355252
3	28.593246446873877	25.139657687383355	33.336718065891375	12.930377799851398
4	24.955533232141068	27.281518948070083	33.358188011527076	14.404759808261769
5	23.09484207974455	31.474977771299738	31.559770942283315	13.870409206672393
6	21.004763480259452	39.66180901340542	28.818324917089665	10.515102589245469
7	85.07306713348498	3.41443327399435	9.671142632005507	1.8413569605151587
8	85.11345910799683	2.985221708100914	9.582265300333814	2.319053883568447
9	79.87621620872234	5.455089782216815	11.611830876779374	3.0568631322814683
10	33.51477248414765	30.579347560950477	24.488065445490133	11.417814509411741
11	33.06986125469164	24.228102997286843	26.425793610499216	16.27624213752231
12	29.156373520100626	23.60910910217034	29.445037501213072	17.789479876515962
13	23.384854957964993	29.415661519749914	30.15425762510927	17.045225897175822
14	18.38513035779121	32.061897890737086	32.174305983070596	17.37866576840111
15	17.684640595732915	27.418282127075848	38.486657346786835	16.4104199304044
16	20.961224079162275	26.135968079099325	36.229802607642924	16.673005234095474
17	20.453027093722493	26.126525799343312	30.433441857101588	22.987005249832606
18	21.745233053824368	27.013725402767562	32.894916793656584	18.346124749751485
19	23.617689586551798	28.535843381055564	29.073641164143165	18.772825868249473
20	25.061983695580903	28.454534860934327	28.37757278704999	18.10590865643478
21	22.487538626230727	28.808058311323204	31.074804962592463	17.62959809985361
22	21.957721817698804	25.44604467772038	30.990086730337108	21.606146774243708
23	19.042305534937	29.665132546002077	30.651813311141478	20.64074860791945
24	19.257454623663328	28.252500049646905	34.007494622209514	18.48255070448025
25	19.890686877142077	28.696849238641253	32.01172641219223	19.400737472024435
26	19.142910777575484	31.754798982481947	29.028677927209767	20.073612312732802
27	19.16520504922163	31.145996754403683	30.727463957281927	18.96133423909276
28	17.763775892735705	29.624403347213235	33.64700187009596	18.9648188899551
29	19.1674532110683	28.283000112033395	32.44052581508045	20.109020861817857
30	20.02074303997194	29.749663431436872	30.794796404589697	19.434797124001488
31	23.423036239994275	28.649263146220072	28.233990183775994	19.693710430009663
32	23.744973016437434	29.294710412399063	28.484547821587373	18.475768749576126
33	23.31639842973389	29.17274763221721	27.7465512260538	19.764302711995104
34	20.54962311440109	29.234234858723635	30.011312001025164	20.204830025850114
35	19.69202430862466	29.7900179365846	30.005279433403263	20.512678321387476
36	23.58936274728375	28.871269128578746	28.088534112296433	19.450834011841067
37	20.080431737001035	31.216663975117342	29.795863157385945	18.907041130495678
38	20.59454888197038	31.34540871020332	27.58771859158655	20.472323816239747
39	20.20865190098945	29.856151364240812	28.887568301967104	21.04762843280263
40	22.27246447623262	27.9810719760256	28.79854109283897	20.94792245490281
41	18.749932086777548	28.037800593289912	29.41517441801647	23.79709290191607
42	20.562062943285998	28.9942435815916	28.06474106608584	22.37895240903656
43	20.645956849530904	28.572151194879286	29.16248102645075	21.619410929139065
44	20.894790896593175	30.096817089926848	28.34531166455027	20.663080348929707
45	19.433448226893486	31.696683998745527	26.718466813571553	22.151400960789434
46	21.482385464584517	29.93745988436205	27.636428764931075	20.94372588612236
47	20.21239883740057	29.527807326534646	28.76020993335324	21.499583902711546
48	20.837687585687753	27.980734751748603	29.94364232944039	21.237935333123257
49	21.03324019698394	27.23550656894157	29.759667751654558	21.971585482419936
50	19.64481290984459	30.013972325877052	29.315018807747318	21.02619595653104
51	18.687021024434898	30.401930121884096	28.3168724171899	22.594176436491114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1922.0
1	2894.5
2	3867.0
3	27554.5
4	51242.0
5	35830.5
6	20419.0
7	19222.0
8	18025.0
9	19969.0
10	21913.0
11	23220.5
12	24528.0
13	25006.0
14	25484.0
15	23857.5
16	22231.0
17	20498.5
18	18766.0
19	18106.0
20	17446.0
21	17036.0
22	16626.0
23	17562.5
24	18499.0
25	19958.5
26	24721.0
27	28024.0
28	33977.5
29	39931.0
30	45069.0
31	50207.0
32	57122.0
33	64037.0
34	71061.5
35	78086.0
36	86474.5
37	94863.0
38	104077.5
39	113292.0
40	133306.5
41	153321.0
42	172002.0
43	190683.0
44	200455.0
45	210227.0
46	220418.0
47	230609.0
48	234295.5
49	237982.0
50	224220.0
51	210458.0
52	191020.5
53	171583.0
54	154206.0
55	136829.0
56	121559.5
57	106290.0
58	96552.5
59	86815.0
60	75156.0
61	63497.0
62	54745.0
63	45993.0
64	37778.0
65	29563.0
66	23592.0
67	17621.0
68	13980.0
69	10339.0
70	8339.0
71	6339.0
72	5328.5
73	4318.0
74	3398.5
75	1999.5
76	1520.0
77	1147.5
78	775.0
79	562.0
80	349.0
81	288.5
82	228.0
83	164.0
84	100.0
85	72.0
86	44.0
87	32.5
88	21.0
89	20.5
90	20.0
91	14.0
92	8.0
93	5.0
94	2.0
95	1.5
96	1.0
97	4.0
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2668847.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.542028143513974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67192249926106	17.814705291514123
2	8.591585955177747	4.045267167084278
3	3.3392717387519824	2.3583968775762005
4	1.8484015158336957	1.7406048202508302
5	1.1818374300137113	1.391142501922051
6	0.833322479878094	1.1770860764347768
7	0.6430905959093931	1.0597759835409675
8	0.5039056564762283	0.94903689171514
9	0.42395410356698754	0.8982665493959032
>10	4.948144722620689	28.220856266733712
>50	1.6768961147329196	27.72699327544823
>100	0.33360507017967617	10.0428835583146
>500	0.0021123011508865216	0.3427396647308132
>1k	0.0017873317430578258	0.8345481323026954
>5k	0.0	0.0
>10k+	1.6248470391434783E-4	1.397696943035623
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36539	1.3690930952579898	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT	3296	0.12349902411041172	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3023	0.1132698877080627	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	2774	0.1039400160443817	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2751	0.10307822066982485	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06081277795242665	0.0
2	0.0	0.0	0.0	0.18963245176662433	0.0
3	0.0	0.0	0.0	0.34449333363808415	0.0
4	0.0	0.0	0.0	0.5468653692025058	0.0
5	0.0	0.0	0.0	1.0594837396074035	0.0
6	0.0	0.0	0.0	1.9085020610023729	0.0
7	0.0	0.0	0.0	2.4751137850914646	0.0
8	0.0	0.0	0.0	3.383521048602636	0.0
9	0.0	0.0	0.0	3.90018611033154	0.0
10	0.0	0.0	0.0	4.545745784602864	0.0
11	0.0	0.0	0.0	5.1131068959741794	0.0
12	0.0	0.0	0.0	5.546589969376289	0.0
13	0.0	0.0	0.0	5.77747619102931	0.0
14	0.0	0.0	0.0	5.911579045183182	0.0
15	0.0	0.0	0.0	6.038000679694265	0.0
16	0.0	0.0	0.0	6.271846981112068	0.0
17	0.0	0.0	0.0	6.5419261576253716	0.0
18	0.0	0.0	0.0	6.860790446211416	0.0
19	3.746936411116861E-5	0.0	0.0	7.0529708147376	0.0
20	1.1240809233350582E-4	0.0	0.0	7.261337948559809	0.0
21	1.1240809233350582E-4	0.0	0.0	7.516242032608089	0.0
22	1.1240809233350582E-4	0.0	0.0	7.7947143466822935	0.0
23	2.2481618466701163E-4	0.0	0.0	8.088998732411412	0.0
24	2.6228554877818025E-4	0.0	0.0	8.3197725459721	0.0
25	3.7469364111168604E-4	0.0	0.0	8.521919765351853	0.0
26	4.871017334451919E-4	0.0	0.0	8.711739563939034	0.0
27	5.620404616675291E-4	0.0	0.0	8.909915030723004	0.0
28	5.995098257786977E-4	0.0	0.0	9.126413016557338	0.0
29	6.369791898898663E-4	0.0	0.0	9.368390169987265	0.0
30	6.74448554001035E-4	0.0	0.0	9.663498881726827	0.0
31	0.002023345662003105	0.0	0.0	9.926271532238454	0.0
32	0.002023345662003105	0.0	0.0	10.197849483316203	0.0
33	0.002023345662003105	0.0	0.0	10.440576024028354	0.0
34	0.002023345662003105	0.0	0.0	10.694768190158522	0.0
35	0.002023345662003105	0.0	0.0	10.969531037185721	0.0
36	0.002060815026114273	0.0	0.0	11.22057577673055	0.0
37	0.002060815026114273	0.0	0.0	11.49440188965497	0.0
38	0.002060815026114273	0.0	0.0	11.787524725096644	0.0
39	0.002060815026114273	0.0	0.0	12.221045267862864	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGA	40	6.8212103E-9	45.000004	14
CGGTCTA	410	0.0	45.000004	31
TATCCGC	20	7.0349645E-4	45.000004	20
CGTTTTT	15910	0.0	43.500942	1
CGACGGT	440	0.0	42.44318	28
GCGCGAC	305	0.0	40.573772	9
TAGGGTA	1935	0.0	39.76744	5
TAACGGG	640	0.0	39.375004	3
TAGGGCG	835	0.0	39.341316	5
TAGTGCG	145	0.0	38.793106	1
ACGGGAT	995	0.0	38.44221	5
ATTAGCG	165	0.0	38.181816	1
GGGCGAT	3785	0.0	38.10436	7
TACGTTG	65	9.094947E-12	38.076927	1
TAAGGGT	1660	0.0	37.680725	4
TGGGCGA	1090	0.0	37.56881	6
TAGCGAT	30	1.1403263E-4	37.500004	28
CGCGATT	30	1.1403263E-4	37.500004	11
CGAGGGT	810	0.0	37.5	4
CGGTTTT	1405	0.0	37.153027	1
>>END_MODULE