FastQCFastQC Report
Thu 26 May 2016
SRR1547925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547925_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1701990
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130380.7660444538452047No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17500.10282081563346436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG351.2123928E-745.09
CGGTCTA2750.044.18181631
CGTTTTT71550.042.075471
GCGTAAG750.042.0000041
GCGATAT1150.041.0869569
AGGGCGA9850.040.888336
CACGACG3100.039.91935326
GGGCGAT19700.039.4035577
CGTAAGG2800.039.3752
TACGGGA8900.038.9325834
ATGGGAC15450.038.8834955
TAGGGAG26550.038.6440665
ACGGTCT3150.038.5714330
TCGTTGA356.250062E-638.57142624
TTAGGGA30450.037.9802934
TAAGGGA30100.037.9734234
CGTTAGG1900.037.8947372
CGACGGT3150.037.85714328
ATAGGGA30100.037.823924
ACGGGTA1250.037.85
Produced by FastQC (version 0.11.5)