FastQCFastQC Report
Thu 26 May 2016
SRR1547924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547924_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2708583
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200830.7414578028437747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG600.044.9999969
CGTTTTT112950.041.892431
CGGTCTA5900.041.567831
GACGGTC6200.039.91935329
CACGACG6300.039.64285726
CGACGGT6250.039.60000228
ACGGTCT6250.039.60000230
TCGCGCA1650.039.54545234
CTCACGA6450.039.41860224
TACGGGA16000.039.3754
CTCGCGC1900.039.07894533
GGGCGAT37200.038.528237
ATAGGGT12100.038.3057824
TAAGGGA41950.038.188324
ACGGGAT14900.038.053695
GCGATAC1250.037.8000039
TAGGGTA16000.037.5468755
TGCGTAG2700.037.5000041
CGAGGGA23400.037.54
TGGGCGA11100.037.56
Produced by FastQC (version 0.11.5)