##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547924_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2708583
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3673740106912	31.0	31.0	33.0	30.0	34.0
2	31.764566934075862	31.0	31.0	34.0	30.0	34.0
3	31.813798580290875	31.0	31.0	34.0	30.0	34.0
4	35.63123337922449	37.0	35.0	37.0	33.0	37.0
5	35.57198542558969	37.0	35.0	37.0	33.0	37.0
6	35.63792580843932	37.0	35.0	37.0	33.0	37.0
7	35.95605746621019	37.0	35.0	37.0	35.0	37.0
8	35.9595519133067	37.0	35.0	37.0	35.0	37.0
9	37.715585603247156	39.0	38.0	39.0	35.0	39.0
10	37.110921467054915	39.0	37.0	39.0	33.0	39.0
11	36.988555639609345	39.0	37.0	39.0	33.0	39.0
12	36.62089476305508	39.0	35.0	39.0	32.0	39.0
13	36.512030091010686	39.0	35.0	39.0	32.0	39.0
14	37.59220448478042	40.0	36.0	41.0	32.0	41.0
15	37.73490862196211	40.0	36.0	41.0	33.0	41.0
16	37.78604643091978	40.0	36.0	41.0	33.0	41.0
17	37.71020603762189	40.0	36.0	41.0	33.0	41.0
18	37.59917528833342	39.0	36.0	41.0	32.0	41.0
19	37.51978802200265	39.0	36.0	41.0	32.0	41.0
20	37.36679031065321	39.0	35.0	41.0	32.0	41.0
21	37.26285330743049	39.0	35.0	41.0	32.0	41.0
22	37.19204211205638	39.0	35.0	41.0	32.0	41.0
23	37.04328019484727	39.0	35.0	41.0	32.0	41.0
24	37.017935946581666	39.0	35.0	41.0	32.0	41.0
25	37.03975879638911	39.0	35.0	41.0	32.0	41.0
26	36.949868621341864	39.0	35.0	41.0	31.0	41.0
27	36.89425651715307	39.0	35.0	41.0	31.0	41.0
28	36.83596662904552	39.0	35.0	41.0	31.0	41.0
29	36.828198729741715	39.0	35.0	41.0	31.0	41.0
30	36.730144507294035	39.0	35.0	41.0	31.0	41.0
31	36.594048622471604	39.0	35.0	41.0	30.0	41.0
32	36.43025965975567	39.0	35.0	41.0	30.0	41.0
33	36.27966283477375	39.0	35.0	41.0	30.0	41.0
34	36.09976840288815	39.0	35.0	41.0	29.0	41.0
35	35.97851976476261	39.0	35.0	41.0	29.0	41.0
36	35.89023633390596	39.0	35.0	41.0	28.0	41.0
37	35.80049162237229	39.0	35.0	41.0	27.0	41.0
38	35.68315610044071	38.0	35.0	41.0	27.0	41.0
39	35.60746929298456	38.0	35.0	41.0	27.0	41.0
40	35.465692208804384	38.0	35.0	40.0	26.0	41.0
41	35.355447848561404	38.0	34.0	40.0	25.0	41.0
42	35.353172489083775	38.0	34.0	40.0	26.0	41.0
43	35.29316952812596	38.0	34.0	40.0	26.0	41.0
44	35.18629593407328	38.0	34.0	40.0	25.0	41.0
45	35.07031647174925	38.0	34.0	40.0	24.0	41.0
46	35.01281887983495	38.0	34.0	40.0	24.0	41.0
47	34.96132110406069	38.0	34.0	40.0	24.0	41.0
48	34.82865690288981	38.0	34.0	40.0	24.0	41.0
49	34.6962223420881	38.0	34.0	40.0	24.0	41.0
50	34.62212344978906	37.0	34.0	40.0	24.0	41.0
51	34.34273862015674	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	4.0
12	6.0
13	13.0
14	22.0
15	77.0
16	173.0
17	460.0
18	999.0
19	1979.0
20	3549.0
21	5780.0
22	8955.0
23	13497.0
24	20680.0
25	30624.0
26	40854.0
27	45724.0
28	48905.0
29	53424.0
30	62011.0
31	74343.0
32	91710.0
33	117457.0
34	182961.0
35	247932.0
36	197966.0
37	262659.0
38	425068.0
39	770637.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.31398114807632	24.701882866428683	28.473633630573623	14.510502354921373
2	32.370099051792025	25.97188271505802	28.56984629970726	13.088171933442688
3	28.935720264064273	25.474390114683583	32.11934062939921	13.470548991852935
4	25.530138821664316	27.72641635866429	31.844805937274213	14.898638882397181
5	23.317210511917118	32.36633324509531	30.173673836098065	14.142782406889506
6	21.650028815805165	40.77560111689396	27.317051018927607	10.257319048373263
7	88.52008596376777	2.8706153734258835	7.280264256255023	1.3290344065513222
8	88.79406686079031	2.5277792853311123	7.03810811778705	1.6400457360915284
9	83.2730988860227	5.7214787215307785	8.704108384347093	2.301314008099438
10	30.118848120954755	40.83186669930366	18.643106007827708	10.406179171913875
11	31.12228792693449	23.969765740979692	28.956026084487718	15.951920247598098
12	29.99734547547555	22.857634416224275	29.268920317376278	17.876099790923888
13	24.439605505904748	27.76577273061228	30.109728961600958	17.684892801882018
14	18.98206553020528	31.244270528169153	30.966154627714936	18.807509313910632
15	17.97386308634441	27.98315576816365	36.5387363060316	17.504244839460338
16	22.260532536754457	26.293342312197925	34.761718581265555	16.68440656978206
17	21.269830018131252	25.768049197680114	30.43344065882419	22.528680125364442
18	22.58258284867032	25.853185964764602	32.577366098805165	18.98686508775991
19	23.54655552368157	27.363348289493068	29.549620594975305	19.540475591850058
20	25.353330505286344	27.47218748696274	29.5928535326405	17.581628475110417
21	23.47179318484979	26.785887676323743	32.06362884209197	17.67869029673449
22	23.2494998307233	23.54064837592202	31.50488650338572	21.70496528996896
23	19.49196314087477	27.819269337509688	32.076366129448495	20.612401392167047
24	19.14883169539202	27.455499794542014	34.66111247098575	18.734556039080214
25	20.11594254265053	28.491945788628225	31.916688541573212	19.475423127148034
26	20.03833738895947	30.34752857859626	29.188546188172932	20.42558784427134
27	18.915794716277848	29.97567362713271	31.304264997602065	19.804266658987373
28	17.44425037002743	29.1928657899721	33.96539814360497	19.397485696395496
29	18.949465458507273	26.784373969710362	33.03125656477944	21.234904007002925
30	20.409417027279577	28.036504696367064	32.394318357606174	19.15975991874718
31	22.55485617387394	27.943725556868664	29.78919235629848	19.712225912958917
32	22.643131113205687	27.97244906284947	30.24167987467986	19.142739949264982
33	22.463479981968433	27.721912158497634	29.725912035924317	20.088695823609616
34	19.259959912618516	28.96174863387978	31.13772035045631	20.640571103045392
35	19.378545903891446	28.655684540588194	31.27938113766497	20.68638841785539
36	23.009854230053133	28.699508193029345	28.905630730164074	19.38500684675345
37	19.714478013042243	30.775833710837	30.26966498719072	19.24002328893004
38	20.449179515636036	30.66167808038373	28.702609445603105	20.18653295837713
39	20.316933245169153	28.768917179204035	29.778596410004788	21.135553165622024
40	21.917844127353675	27.909427180189788	29.41674669005897	20.755982002397563
41	19.010198321410122	27.760419377955188	30.53670498559579	22.692677315038896
42	20.741324892019186	28.10753814817563	29.162222460969446	21.988914498835737
43	20.6012516507709	27.791099626631343	29.923173851419726	21.68447487117803
44	20.513604345888606	29.19094596695025	29.321161655374784	20.97428803178636
45	19.910410720291754	30.154069489471063	27.94169497482632	21.99382481541086
46	20.926920090689485	29.21811884664417	29.036215615323584	20.818745447342764
47	20.157587934355345	28.981869855935745	29.993801186819823	20.86674102288909
48	20.233937819147503	28.731259112236916	30.35853064129842	20.676272427317162
49	21.169113148830956	27.132378812094736	30.279190262952994	21.419317776121318
50	19.90077468550899	29.56867114649985	29.838775477805186	20.691778690185973
51	19.352702132443422	29.073172208494256	28.975962708176194	22.598162950886127
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2168.0
1	3274.0
2	4380.0
3	19195.0
4	34010.0
5	25032.5
6	16055.0
7	15057.0
8	14059.0
9	14593.5
10	15128.0
11	15350.5
12	15573.0
13	15552.5
14	15532.0
15	15205.5
16	14879.0
17	14543.0
18	14207.0
19	14330.0
20	14453.0
21	15162.5
22	15872.0
23	17866.5
24	19861.0
25	22374.0
26	29431.5
27	33976.0
28	41246.0
29	48516.0
30	58140.5
31	67765.0
32	75152.5
33	82540.0
34	93035.0
35	103530.0
36	110944.0
37	118358.0
38	128088.0
39	137818.0
40	155751.5
41	173685.0
42	187462.0
43	201239.0
44	213182.0
45	225125.0
46	225002.5
47	224880.0
48	218477.0
49	212074.0
50	198812.5
51	185551.0
52	172694.0
53	159837.0
54	145385.5
55	130934.0
56	118636.5
57	106339.0
58	96066.0
59	85793.0
60	76457.5
61	67122.0
62	56934.0
63	46746.0
64	38835.0
65	30924.0
66	24385.0
67	17846.0
68	14153.0
69	10460.0
70	8394.0
71	6328.0
72	5160.5
73	3993.0
74	3409.5
75	2192.0
76	1558.0
77	1203.0
78	848.0
79	650.0
80	452.0
81	341.0
82	230.0
83	173.5
84	117.0
85	85.0
86	53.0
87	36.5
88	20.0
89	17.5
90	15.0
91	12.5
92	10.0
93	7.0
94	4.0
95	4.5
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2708583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.823309488916486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25885393500144	16.642205706936135
2	8.069750661741008	3.522173323791252
3	2.885438376786773	1.889094441234437
4	1.54913980245395	1.3522942940220601
5	0.9542896057919307	1.0412878704626707
6	0.7035166240233852	0.9211836609996021
7	0.5389817158924006	0.8233655356350981
8	0.43660906006239364	0.7622603714725235
9	0.35718519834400314	0.7015466815489081
>10	5.979957617716732	33.735138637010905
>50	2.0182564990284213	30.020457869897925
>100	0.24388460843776577	7.059180781316381
>500	0.002929875426589027	0.4385502834080109
>1k	0.0010340736799725977	0.33590845464124747
>5k	0.0	0.0
>10k+	1.723456133287663E-4	0.755352087622906
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20083	0.7414578028437747	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050358434650147325	0.0
2	0.0	0.0	0.0	0.1471618185597414	0.0
3	0.0	0.0	0.0	0.23510448083001334	0.0
4	0.0	0.0	0.0	0.38241397808374344	0.0
5	0.0	0.0	0.0	0.6818325301458364	0.0
6	0.0	0.0	0.0	1.0595946293689358	0.0
7	0.0	0.0	0.0	1.2733595389175816	0.0
8	0.0	0.0	0.0	1.7508047565830547	0.0
9	0.0	0.0	0.0	1.9331510239856042	0.0
10	0.0	0.0	0.0	2.2850693517606806	0.0
11	0.0	0.0	0.0	2.6753841399728198	0.0
12	0.0	0.0	0.0	2.9868015859214947	0.0
13	0.0	0.0	0.0	3.1251396025154112	0.0
14	0.0	0.0	0.0	3.1891952360330107	0.0
15	0.0	0.0	0.0	3.287955362637955	0.0
16	0.0	0.0	0.0	3.4833342747850073	0.0
17	0.0	0.0	0.0	3.727964031377292	0.0
18	0.0	0.0	0.0	4.013316187836962	0.0
19	0.0	0.0	0.0	4.176574984041471	0.0
20	0.0	0.0	0.0	4.365640632020507	0.0
21	0.0	0.0	0.0	4.598160735705718	0.0
22	0.0	0.0	0.0	4.856856887900427	0.0
23	0.0	0.0	0.0	5.131317740678429	0.0
24	0.0	0.0	0.0	5.331570049727108	0.0
25	0.0	0.0	0.0	5.519269669786748	0.0
26	0.0	0.0	0.0	5.700176069922908	0.0
27	7.383934699435092E-5	0.0	0.0	5.89869315431722	0.0
28	7.383934699435092E-5	0.0	0.0	6.10861841782216	0.0
29	7.383934699435092E-5	0.0	0.0	6.346159597102987	0.0
30	7.383934699435092E-5	0.0	0.0	6.621506522044922	0.0
31	7.383934699435092E-5	0.0	0.0	6.8700128443544095	0.0
32	7.383934699435092E-5	0.0	0.0	7.102902144774593	0.0
33	7.383934699435092E-5	0.0	0.0	7.339778769932471	0.0
34	7.383934699435092E-5	0.0	0.0	7.589761879181845	0.0
35	7.383934699435092E-5	0.0	0.0	7.8717543453532715	0.0
36	7.383934699435092E-5	0.0	0.0	8.121072900479698	0.0
37	7.383934699435092E-5	0.0	0.0	8.382538028186694	0.0
38	7.383934699435092E-5	0.0	0.0	8.661023125375888	0.0
39	7.383934699435092E-5	0.0	0.0	9.044581613338044	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	60	0.0	44.999996	9
CGTTTTT	11295	0.0	41.89243	1
CGGTCTA	590	0.0	41.5678	31
GACGGTC	620	0.0	39.919353	29
CACGACG	630	0.0	39.642857	26
CGACGGT	625	0.0	39.600002	28
ACGGTCT	625	0.0	39.600002	30
TCGCGCA	165	0.0	39.545452	34
CTCACGA	645	0.0	39.418602	24
TACGGGA	1600	0.0	39.375	4
CTCGCGC	190	0.0	39.078945	33
GGGCGAT	3720	0.0	38.52823	7
ATAGGGT	1210	0.0	38.305782	4
TAAGGGA	4195	0.0	38.18832	4
ACGGGAT	1490	0.0	38.05369	5
GCGATAC	125	0.0	37.800003	9
TAGGGTA	1600	0.0	37.546875	5
TGCGTAG	270	0.0	37.500004	1
CGAGGGA	2340	0.0	37.5	4
TGGGCGA	1110	0.0	37.5	6
>>END_MODULE