FastQCFastQC Report
Thu 26 May 2016
SRR1547923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547923_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2667146
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT532751.9974534577409708No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA75130.28168686678569527No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42170.15810907989288925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT233650.043.651831
CGACGGT4100.040.60975628
ACGGTCT4100.040.60975630
TACGGGA12350.040.0809754
GCGAGAC7950.039.90566321
CGGTTTT18550.039.663071
ACACGAC7950.039.62264326
CGGTCTA4150.039.57831231
CACGACC8100.038.8888927
TAGGGAT49100.038.3095745
AGGGCGA18100.038.287296
GGGCGAT34450.038.2728587
GACACGA8250.038.18181625
TAGGGAC35400.038.1355935
ATAGGGA47350.038.0623054
ACGACCA8350.037.7245528
GCTCAAC18850.037.599471
AGACACG8500.037.58823824
TACCGCG301.1403263E-437.4999961
AGGGATT57500.037.4478266