##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547923_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2667146
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34012048834222	31.0	31.0	33.0	30.0	34.0
2	31.696072131034448	31.0	31.0	34.0	30.0	34.0
3	31.524257014801588	31.0	31.0	34.0	30.0	34.0
4	35.479153747113955	37.0	35.0	37.0	33.0	37.0
5	35.489267179224534	37.0	35.0	37.0	33.0	37.0
6	35.59322811724593	37.0	35.0	37.0	33.0	37.0
7	35.91375350280787	37.0	35.0	37.0	35.0	37.0
8	35.93338084979225	37.0	35.0	37.0	35.0	37.0
9	37.7163627337986	39.0	38.0	39.0	35.0	39.0
10	37.24183153078234	39.0	37.0	39.0	34.0	39.0
11	37.01487245167681	39.0	37.0	39.0	33.0	39.0
12	36.734426611816524	39.0	35.0	39.0	32.0	39.0
13	36.6696952472793	39.0	35.0	39.0	32.0	39.0
14	37.847876344227124	40.0	36.0	41.0	33.0	41.0
15	37.95747101958423	40.0	37.0	41.0	33.0	41.0
16	37.97582022131522	40.0	36.0	41.0	33.0	41.0
17	37.884599118308486	40.0	36.0	41.0	33.0	41.0
18	37.697034208101094	39.0	36.0	41.0	33.0	41.0
19	37.52462069942928	39.0	36.0	41.0	33.0	41.0
20	37.29154272019604	39.0	35.0	41.0	32.0	41.0
21	37.17510814931016	39.0	35.0	41.0	32.0	41.0
22	37.09675248374105	39.0	35.0	41.0	32.0	41.0
23	36.952912588962135	39.0	35.0	41.0	32.0	41.0
24	36.898939540617576	38.0	35.0	41.0	32.0	41.0
25	36.884984174094704	39.0	35.0	41.0	32.0	41.0
26	36.77939040457478	38.0	35.0	41.0	31.0	41.0
27	36.700750165157814	38.0	35.0	41.0	31.0	41.0
28	36.600827626234185	38.0	35.0	41.0	31.0	41.0
29	36.579333114872604	38.0	35.0	41.0	31.0	41.0
30	36.46700143149269	38.0	35.0	40.0	31.0	41.0
31	36.284584345963815	38.0	35.0	40.0	30.0	41.0
32	35.991821220135684	38.0	35.0	40.0	30.0	41.0
33	35.69227518853486	38.0	35.0	41.0	27.0	41.0
34	35.376196503678464	38.0	35.0	40.0	25.0	41.0
35	35.13005549752432	38.0	34.0	41.0	24.0	41.0
36	34.96623844363975	38.0	34.0	40.0	23.0	41.0
37	34.84313307183034	38.0	34.0	40.0	23.0	41.0
38	34.698025529911	38.0	34.0	40.0	22.0	41.0
39	34.586265243822425	38.0	34.0	40.0	22.0	41.0
40	34.44095936255458	38.0	34.0	40.0	21.0	41.0
41	34.30923391520374	38.0	33.0	40.0	20.0	41.0
42	34.27069946677085	38.0	33.0	40.0	20.0	41.0
43	34.17635292556163	38.0	33.0	40.0	20.0	41.0
44	33.99271955865933	37.0	33.0	40.0	18.0	41.0
45	33.87127326363086	37.0	33.0	40.0	19.0	41.0
46	33.81144264318489	37.0	33.0	40.0	19.0	41.0
47	33.76632550299084	37.0	33.0	40.0	19.0	41.0
48	33.65125268732945	37.0	33.0	40.0	19.0	41.0
49	33.50813566261464	36.0	33.0	40.0	18.0	41.0
50	33.428880908656666	36.0	33.0	40.0	18.0	41.0
51	33.14932665853313	36.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	4.0
12	5.0
13	6.0
14	35.0
15	73.0
16	187.0
17	550.0
18	1249.0
19	2391.0
20	4319.0
21	7143.0
22	11009.0
23	16838.0
24	26378.0
25	41784.0
26	57796.0
27	63466.0
28	61791.0
29	61729.0
30	66284.0
31	75367.0
32	90308.0
33	113326.0
34	171414.0
35	242041.0
36	209743.0
37	262176.0
38	403911.0
39	675714.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.642004599673207	23.08598779369408	27.364193786166936	18.907813820465773
2	35.8043016767736	23.888718502849112	27.23525446301027	13.071725357367015
3	27.072608698586432	24.303918870583015	35.95539951693683	12.668072913893727
4	23.28957619867829	26.563825152428848	35.496706966922694	14.649891681970164
5	21.778560303785394	31.132941353791654	33.77719104990878	13.31130729251417
6	20.24096168713674	38.86536395082984	30.84206863816229	10.051605723871134
7	83.42340464301542	2.550254091826994	11.995406325712953	2.0309349394446348
8	83.61345798092793	2.3605381932597616	12.40310804132957	1.622895784482739
9	78.69452965829392	5.460181032459415	13.62366364645955	2.221625662787114
10	32.284246906618534	40.90563471216049	17.629256141208618	9.180862240012358
11	26.178544406642906	24.64000845847959	33.27556871652321	15.9058784183543
12	25.797612879084987	22.628570014539886	33.01180362829781	18.562013478077315
13	22.354681745956164	26.597194154350756	33.327571868956554	17.720552230736526
14	17.572866277286657	29.54663899164125	33.96023314809163	18.920261582980462
15	16.758587643871014	26.91959870213329	39.01454963470316	17.307264019292532
16	20.88423355901777	26.171008261265037	37.006485584216236	15.938272595500958
17	19.63402078476394	25.116847746617545	33.47180844243247	21.777323026186043
18	20.69916682476325	26.01184937007573	35.13260991336807	18.156373891792953
19	21.64650904000006	27.3800159421344	32.66540339373998	18.308071624125564
20	23.773314246764144	27.342635161329753	31.95726818104446	16.926782410861648
21	21.858533428616205	27.575843242177218	33.64067808811366	16.924945241092914
22	22.4629997757903	23.675869262500065	33.39198529064401	20.469145671065625
23	18.43041213341902	28.517523975065483	33.662836605120226	19.38922728639527
24	18.155998959187087	27.4407175310238	36.71583782815039	17.687445681638724
25	19.133523249195957	29.14628595509957	33.66621099857301	18.05397979713147
26	19.45918971064951	30.293917168388983	31.56565107421941	18.681242046742096
27	18.639699514012356	28.951845905698452	33.67910868021473	18.729345900074463
28	17.590225656938166	28.244385571693485	36.07207854388174	18.093310227486608
29	18.05371734430736	27.00639560039083	35.56067046948311	19.3792165858187
30	19.01830645941392	27.275147292274216	34.90198886750107	18.8045573808108
31	21.97052579798781	27.909833207480954	31.49486379823227	18.624777196298965
32	21.157746894995626	28.55434235696134	31.918612629379865	18.36929811866317
33	20.301963222110825	28.26545678414305	31.14970084127378	20.282879152472344
34	18.217862839154662	29.349761880302015	32.68737444444361	19.74500083609971
35	18.955992660319307	28.90569170191658	32.41183647239409	19.726479165370026
36	20.71847585396525	29.562911066735754	30.234077924493075	19.484535154805922
37	18.721022396224278	31.099647338390923	30.3650418837214	19.814288381663395
38	19.216045915746644	31.212839492101295	29.408476326380335	20.162638265771726
39	19.365344079401726	29.942230384088457	29.61352696852741	21.078898567982403
40	21.07604908017784	28.124107191732286	30.384013473578126	20.415830254511754
41	18.794021774586017	28.346629693312625	30.58257778164375	22.276770750457604
42	20.620281004489442	29.032643882262164	28.661835535062576	21.685239578185822
43	20.320972305228135	29.169944202529596	29.671604029175757	20.837479463066515
44	20.243623708638374	30.007206204684707	29.725781790723115	20.023388295953804
45	19.225869150020284	30.590038940500445	28.956795016095853	21.227296893383414
46	20.27268098559284	29.927757985502108	29.259740561634047	20.539820467271007
47	19.783618894503714	29.404127108152313	30.348657328845142	20.463596668498838
48	19.773645687187727	28.76201752734946	31.005051841931415	20.4592849435314
49	20.045809265784477	28.131230911243705	30.491131719073493	21.331828103898324
50	18.609817385324988	29.864206908808143	31.346915391958298	20.179060313908575
51	18.644423664846244	29.628786725586075	30.137307818919552	21.589481790648133
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2303.0
1	3249.5
2	4196.0
3	41464.5
4	78733.0
5	55272.0
6	31811.0
7	29374.5
8	26938.0
9	28091.5
10	29245.0
11	30454.0
12	31663.0
13	31588.5
14	31514.0
15	29787.5
16	28061.0
17	25962.5
18	23864.0
19	22295.5
20	20727.0
21	20686.5
22	20646.0
23	21722.0
24	22798.0
25	25238.5
26	31011.0
27	34343.0
28	39608.5
29	44874.0
30	54934.5
31	64995.0
32	69676.0
33	74357.0
34	80837.5
35	87318.0
36	95393.0
37	103468.0
38	110992.5
39	118517.0
40	133536.5
41	148556.0
42	159787.0
43	171018.0
44	179945.5
45	188873.0
46	193976.5
47	199080.0
48	199327.5
49	199575.0
50	192081.0
51	184587.0
52	168910.5
53	153234.0
54	136811.5
55	120389.0
56	111054.5
57	101720.0
58	90770.5
59	79821.0
60	70926.5
61	62032.0
62	56881.5
63	51731.0
64	44858.0
65	37985.0
66	30912.5
67	23840.0
68	19090.0
69	14340.0
70	11385.5
71	8431.0
72	7035.5
73	5640.0
74	4910.0
75	3270.0
76	2360.0
77	1618.5
78	877.0
79	693.5
80	510.0
81	339.5
82	169.0
83	115.5
84	62.0
85	51.5
86	41.0
87	31.0
88	21.0
89	17.5
90	14.0
91	10.0
92	6.0
93	4.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2667146.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.28609938097372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.06240277708135	18.980353763959567
2	7.465188958815933	3.775310198207348
3	3.0525661115962186	2.315624701944435
4	1.922302027106423	1.94430080390641
5	1.2999497594873475	1.6435329404334977
6	1.0354192578847825	1.5709028553509201
7	0.7700968968863398	1.363092266735322
8	0.6180106463016067	1.2501662896705783
9	0.5180200981778704	1.1788836915480656
>10	7.166056213628	42.775944454888055
>50	1.0121809704212983	16.637547269533044
>100	0.07236879942653843	2.827555431354636
>500	0.003473947591228862	0.5969858395949535
>1k	0.0016614531958051083	0.8181581892267349
>5k	1.5104119961864622E-4	0.28693971037533655
>10k+	1.5104119961864622E-4	2.034701593271137
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53275	1.9974534577409708	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	7513	0.28168686678569527	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4217	0.15810907989288925	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1247978175922878E-4	0.0	0.0	0.0520031524333501	0.0
2	1.1247978175922878E-4	0.0	0.0	0.13909999677557958	0.0
3	1.1247978175922878E-4	0.0	0.0	0.26136551954786125	0.0
4	1.1247978175922878E-4	0.0	0.0	0.3783069993168728	0.0
5	1.1247978175922878E-4	0.0	0.0	0.6953500108355523	0.0
6	1.1247978175922878E-4	0.0	0.0	1.2206305916511506	0.0
7	1.1247978175922878E-4	0.0	0.0	1.5628690742838975	0.0
8	1.1247978175922878E-4	0.0	0.0	2.133591486930224	0.0
9	1.1247978175922878E-4	0.0	0.0	2.4478599971655095	0.0
10	1.1247978175922878E-4	0.0	0.0	2.8490753787006784	0.0
11	1.1247978175922878E-4	0.0	0.0	3.207735909470273	0.0
12	1.1247978175922878E-4	0.0	0.0	3.4935845281810596	0.0
13	1.1247978175922878E-4	0.0	0.0	3.668828028161938	0.0
14	1.1247978175922878E-4	0.0	0.0	3.754875061207748	0.0
15	1.1247978175922878E-4	0.0	0.0	3.8725664061884877	0.0
16	1.1247978175922878E-4	0.0	0.0	4.0183776966090345	0.0
17	1.4997304234563838E-4	0.0	0.0	4.200070037410775	0.0
18	1.4997304234563838E-4	0.0	0.0	4.421355261391765	0.0
19	1.4997304234563838E-4	0.0	0.0	4.551569355408366	0.0
20	1.4997304234563838E-4	0.0	0.0	4.700830025802862	0.0
21	1.4997304234563838E-4	0.0	0.0	4.872586652549204	0.0
22	1.4997304234563838E-4	0.0	0.0	5.066689262605046	0.0
23	1.4997304234563838E-4	0.0	0.0	5.286174810077888	0.0
24	1.4997304234563838E-4	0.0	0.0	5.45125763643985	0.0
25	1.4997304234563838E-4	0.0	0.0	5.623089249707365	0.0
26	1.4997304234563838E-4	0.0	0.0	5.774974448342911	0.0
27	1.4997304234563838E-4	0.0	0.0	5.936045495822126	0.0
28	1.4997304234563838E-4	0.0	0.0	6.102815518910476	0.0
29	1.4997304234563838E-4	0.0	0.0	6.282595703422309	0.0
30	1.4997304234563838E-4	0.0	0.0	6.517415994474993	0.0
31	1.4997304234563838E-4	0.0	0.0	6.715455396892408	0.0
32	1.4997304234563838E-4	0.0	0.0	6.924255365098124	0.0
33	1.4997304234563838E-4	0.0	0.0	7.120982503395015	0.0
34	1.4997304234563838E-4	0.0	0.0	7.340992956516066	0.0
35	1.4997304234563838E-4	0.0	0.0	7.565090175041036	0.0
36	1.4997304234563838E-4	0.0	0.0	7.766541464171815	0.0
37	1.4997304234563838E-4	0.0	0.0	7.980365529296109	0.0
38	1.4997304234563838E-4	0.0	0.0	8.19568932484386	0.0
39	1.4997304234563838E-4	0.0	0.0	8.45529266114416	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	23365	0.0	43.65183	1
CGACGGT	410	0.0	40.609756	28
ACGGTCT	410	0.0	40.609756	30
TACGGGA	1235	0.0	40.080975	4
GCGAGAC	795	0.0	39.905663	21
CGGTTTT	1855	0.0	39.66307	1
ACACGAC	795	0.0	39.622643	26
CGGTCTA	415	0.0	39.578312	31
CACGACC	810	0.0	38.88889	27
TAGGGAT	4910	0.0	38.309574	5
AGGGCGA	1810	0.0	38.28729	6
GGGCGAT	3445	0.0	38.272858	7
GACACGA	825	0.0	38.181816	25
TAGGGAC	3540	0.0	38.135593	5
ATAGGGA	4735	0.0	38.062305	4
ACGACCA	835	0.0	37.72455	28
GCTCAAC	1885	0.0	37.59947	1
AGACACG	850	0.0	37.588238	24
TACCGCG	30	1.1403263E-4	37.499996	1
AGGGATT	5750	0.0	37.447826	6
>>END_MODULE