FastQCFastQC Report
Thu 26 May 2016
SRR1547922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547922_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2237114
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199330.8910140475630657No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37000.1653916608630584No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT29390.13137461926392666Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC22800.10191702345074949No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC207.0347206E-445.026
TATCGTG207.0347206E-445.01
AATCGCG453.8562575E-1045.01
GTAGCGT253.891995E-544.99999610
TCGACCG253.891995E-544.99999619
CGACGGT8300.044.1867528
CGTTTTT90900.042.995051
CGGTCTA8600.042.64534831
TATAGCG950.042.631581
TCACGAC8850.042.20339225
GACGGTC8750.042.1714329
TACGGGT3950.042.1518974
ACGGTCT8750.041.91428830
CACGACG8800.041.67613626
GCTAGCG603.6379788E-1241.2500041
GACGGGT5300.040.754724
TAGGGCG9800.040.6377565
CTCACGA9200.040.59782424
AAGGGTC12650.040.553365
CTCGCAG501.0822987E-940.4999961
Produced by FastQC (version 0.11.5)