##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547922_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2237114
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268898679280536	31.0	31.0	33.0	30.0	34.0
2	31.645266177762956	31.0	31.0	34.0	30.0	34.0
3	31.69181588421511	31.0	31.0	34.0	30.0	34.0
4	35.53062472453348	37.0	35.0	37.0	33.0	37.0
5	35.45669375811872	37.0	35.0	37.0	33.0	37.0
6	35.52256076355519	37.0	35.0	37.0	33.0	37.0
7	35.870260970160665	37.0	35.0	37.0	35.0	37.0
8	35.877352249371285	37.0	35.0	37.0	35.0	37.0
9	37.63223286788246	39.0	37.0	39.0	35.0	39.0
10	36.94756950249295	39.0	37.0	39.0	32.0	39.0
11	36.82425705618936	39.0	37.0	39.0	32.0	39.0
12	36.32843565415084	38.0	35.0	39.0	32.0	39.0
13	36.15256531406089	38.0	35.0	39.0	32.0	39.0
14	37.12052224428438	39.0	35.0	41.0	31.0	41.0
15	37.32504914814355	39.0	35.0	41.0	32.0	41.0
16	37.43623570367894	39.0	35.0	41.0	32.0	41.0
17	37.39589533658097	39.0	35.0	41.0	32.0	41.0
18	37.294289875258926	39.0	35.0	41.0	32.0	41.0
19	37.21610163809265	39.0	35.0	41.0	32.0	41.0
20	37.027761213778106	39.0	35.0	41.0	32.0	41.0
21	36.92301912195802	39.0	35.0	41.0	32.0	41.0
22	36.854352080403594	38.0	35.0	41.0	31.0	41.0
23	36.73011433480815	38.0	35.0	40.0	31.0	41.0
24	36.66234174923584	38.0	35.0	40.0	31.0	41.0
25	36.67547474111735	38.0	35.0	40.0	31.0	41.0
26	36.57277054276179	38.0	35.0	40.0	31.0	41.0
27	36.49331862390562	38.0	35.0	40.0	31.0	41.0
28	36.42787046167518	38.0	35.0	40.0	31.0	41.0
29	36.403390260845	38.0	35.0	40.0	31.0	41.0
30	36.298162275145565	38.0	35.0	40.0	30.0	41.0
31	36.16190860188618	38.0	35.0	40.0	30.0	41.0
32	35.980749304684515	38.0	35.0	40.0	30.0	41.0
33	35.820225969709185	38.0	35.0	40.0	29.0	41.0
34	35.60922152380254	38.0	35.0	40.0	28.0	41.0
35	35.49488716265689	38.0	34.0	40.0	27.0	41.0
36	35.35966785778463	38.0	34.0	40.0	27.0	41.0
37	35.224156211976684	38.0	34.0	40.0	26.0	41.0
38	35.099756650756284	38.0	34.0	40.0	25.0	41.0
39	35.033114986540696	38.0	34.0	40.0	25.0	41.0
40	34.843937769823086	38.0	34.0	40.0	24.0	41.0
41	34.74178383399326	37.0	34.0	40.0	24.0	41.0
42	34.73823685337448	37.0	34.0	40.0	24.0	41.0
43	34.67331034538249	37.0	34.0	40.0	24.0	41.0
44	34.5615999005862	37.0	34.0	40.0	24.0	41.0
45	34.465855562121554	37.0	33.0	40.0	23.0	41.0
46	34.40116104945926	37.0	33.0	40.0	23.0	41.0
47	34.316836781674965	37.0	33.0	40.0	23.0	41.0
48	34.15272534166788	37.0	33.0	40.0	23.0	41.0
49	33.99398868363436	36.0	33.0	40.0	23.0	41.0
50	33.87289382659981	36.0	33.0	40.0	22.0	41.0
51	33.53922553790285	36.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	3.0
12	4.0
13	11.0
14	22.0
15	58.0
16	154.0
17	366.0
18	868.0
19	1868.0
20	3195.0
21	5213.0
22	8257.0
23	12505.0
24	19251.0
25	27984.0
26	36588.0
27	40744.0
28	44110.0
29	49842.0
30	58549.0
31	70298.0
32	86506.0
33	110872.0
34	176337.0
35	248394.0
36	178276.0
37	211142.0
38	318041.0
39	527578.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.332824344222065	25.11579651282858	28.972148938319638	14.579230204629715
2	30.61319181767223	26.305454259371675	29.824899401639794	13.256454521316302
3	27.597029029365512	25.855186637784218	32.92585894147549	13.62192539137478
4	25.28145637638493	27.509729052699143	32.6666410384093	14.542173532506613
5	22.237489908873663	32.450067363576466	31.309311908110182	14.003130819439688
6	20.397842935138755	41.412954368887775	27.77475801411998	10.414444681853494
7	86.12904840790411	3.5328552769326906	8.304494093729689	2.0336022214335077
8	85.92789638793553	3.0514314424745455	8.012734263877478	3.007937905712449
9	80.96985669930098	5.34317875620107	10.328932723142406	3.35803182135555
10	35.62688356516476	29.214291269912934	22.871833978956815	12.28699118596549
11	33.917225496778435	24.196978786060967	25.334605210105522	16.551190507055072
12	30.61314711722335	23.517397861709327	28.211704901940628	17.657750119126696
13	22.823512793715476	31.32451006073003	29.577393016180668	16.274584129373824
14	17.23814700547223	34.9154759212092	30.434524123491247	17.41185294982732
15	15.969101261714872	28.34366062704002	39.361561368799265	16.325676742445847
16	18.723185318226964	26.235810960013662	37.54497982668742	17.496023895071954
17	18.70619914765184	26.911905249352515	28.926822683153386	25.45507291984226
18	19.95727531095867	28.086275442377996	32.862160801818774	19.094288444844562
19	22.898564847388197	30.54202870305224	26.925315384017086	19.63409106554248
20	23.10248829518746	29.804560697398525	27.90327180465546	19.18967920275855
21	20.331462768549123	30.371854094158813	31.116384770735866	18.180298366556197
22	20.355735112292	26.78509007587454	29.445660793325683	23.413514018507776
23	17.049421710292815	31.354369960583146	29.158594510606072	22.437613818517967
24	18.336794638091757	29.423042366191442	33.83761399731976	18.40254899839704
25	18.70686965438507	29.788200333107746	31.314810063322657	20.190119949184528
26	17.36907462024734	33.202912323645556	28.050738585516875	21.377274470590233
27	17.721001254294595	32.643620307235125	29.710153349359935	19.925225089110345
28	16.190636686373605	30.737235563319526	32.905207334092054	20.166920416214822
29	16.698433785672076	28.565061950352106	32.31395449673106	22.422549767244764
30	18.756442452195106	30.756724959032038	30.63938628071703	19.847446308055826
31	21.96995772231545	29.11322355499094	27.626352523832043	21.29046619886157
32	22.881131672324255	28.664252246421057	28.163115514005995	20.291500567248697
33	22.117379802727978	29.279106920791698	26.44438325449664	22.15913002198368
34	17.704640890003816	29.70215196901007	29.769560245923987	22.82364689506212
35	18.323786807467123	29.03964661613132	29.466088898464722	23.170477677936844
36	24.27180733748928	28.184839932162596	27.23772682125274	20.30562590909538
37	18.9681884785487	30.8143885380897	29.808583737797896	20.408839245563705
38	19.90144445030517	31.732222854981913	26.154098539457532	22.212234155255388
39	19.91083154457037	30.296310335548387	28.23977678383846	21.553081336042776
40	21.834425961305502	28.66545915854087	28.274821935761878	21.225292944391747
41	19.28793078940099	26.826795594681364	29.064589466607423	24.820684149310228
42	21.18533074309132	27.832466293626524	28.2888578767108	22.69334508657136
43	20.39019915837995	27.945469028399984	28.690402009016974	22.973929804203095
44	19.785983190843204	30.197030638581673	27.734929914166194	22.28205625640893
45	18.010794264396	32.24484760275963	26.16858148489527	23.575776647949098
46	21.41772837682836	30.573274316820694	27.25627750753873	20.75271979881222
47	20.02615870268569	28.833979850825664	28.83947800603814	22.300383440450506
48	20.157622722847382	28.56376563733453	28.890794121354567	22.38781751846352
49	20.989453376090804	26.36982290576162	29.20763090302953	23.43309281511805
50	19.0309479087789	29.422461260355977	29.296987100344463	22.24960373052066
51	17.79064455365261	30.284017712105864	27.4364650169817	24.488872717259827
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1886.0
1	2584.5
2	3283.0
3	15272.5
4	27262.0
5	19534.5
6	11807.0
7	11855.0
8	11903.0
9	13999.5
10	16096.0
11	17608.5
12	19121.0
13	19487.0
14	19853.0
15	18441.5
16	17030.0
17	15661.5
18	14293.0
19	13681.5
20	13070.0
21	12858.0
22	12646.0
23	13583.5
24	14521.0
25	16795.0
26	21506.0
27	23943.0
28	28211.5
29	32480.0
30	39543.0
31	46606.0
32	51345.0
33	56084.0
34	63720.5
35	71357.0
36	77880.5
37	84404.0
38	93027.0
39	101650.0
40	117264.5
41	132879.0
42	149659.0
43	166439.0
44	172067.0
45	177695.0
46	182308.5
47	186922.0
48	203266.5
49	219611.0
50	214382.0
51	209153.0
52	188335.0
53	167517.0
54	140794.5
55	114072.0
56	98467.0
57	82862.0
58	70749.5
59	58637.0
60	49158.0
61	39679.0
62	32229.5
63	24780.0
64	20442.5
65	16105.0
66	12244.0
67	8383.0
68	7073.0
69	5763.0
70	4650.0
71	3537.0
72	3018.0
73	2499.0
74	1726.5
75	721.0
76	488.0
77	421.0
78	354.0
79	280.5
80	207.0
81	150.0
82	93.0
83	76.0
84	59.0
85	40.5
86	22.0
87	19.0
88	16.0
89	15.5
90	15.0
91	9.0
92	3.0
93	4.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2237114.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.10577399004024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.18195893905109	18.875012692523022
2	9.571052292120596	4.805773513856726
3	3.749830127716543	2.824271630624858
4	1.9458485211361172	1.9540813276198963
5	1.2168492293675817	1.5274970866228577
6	0.8630914213364033	1.3001146894088604
7	0.609946869305937	1.0719231772709228
8	0.4744050022669385	0.9528243813326629
9	0.3899809750884895	0.8811696798888418
>10	4.209080084081796	24.980890143894513
>50	1.380331849587351	24.580064229057736
>100	0.4009512339210144	13.485688646005462
>500	0.0043287275805476925	0.7947842250863164
>1k	0.0021643637902738462	1.0633046475317276
>5k	0.0	0.0
>10k+	1.8036364918948722E-4	0.9025999292756122
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19933	0.8910140475630657	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3700	0.1653916608630584	No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	2939	0.13137461926392666	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	2280	0.10191702345074949	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.470044888190767E-5	0.0	0.0	0.16762668330715377	0.0
2	4.470044888190767E-5	0.0	0.0	0.5190616124167119	0.0
3	4.470044888190767E-5	0.0	0.0	0.845777193294575	0.0
4	4.470044888190767E-5	0.0	0.0	1.2751697052541802	0.0
5	4.470044888190767E-5	0.0	0.0	2.277532570982078	0.0
6	4.470044888190767E-5	0.0	0.0	3.377342415272534	0.0
7	4.470044888190767E-5	0.0	0.0	4.080033471696122	0.0
8	4.470044888190767E-5	0.0	0.0	5.492344154119996	0.0
9	4.470044888190767E-5	0.0	0.0	6.073628791380323	0.0
10	4.470044888190767E-5	0.0	0.0	7.059005486533096	0.0
11	4.470044888190767E-5	0.0	0.0	8.08353977490642	0.0
12	4.470044888190767E-5	0.0	0.0	8.852298094777467	0.0
13	4.470044888190767E-5	0.0	0.0	9.206727953962114	0.0
14	4.470044888190767E-5	0.0	0.0	9.361212705297987	0.0
15	4.470044888190767E-5	0.0	0.0	9.569695598883204	0.0
16	4.470044888190767E-5	0.0	0.0	9.993098250692633	0.0
17	4.470044888190767E-5	0.0	0.0	10.473717477070904	0.0
18	4.470044888190767E-5	0.0	0.0	11.03573622086313	0.0
19	4.470044888190767E-5	0.0	0.0	11.337419550367125	0.0
20	4.470044888190767E-5	0.0	0.0	11.65130610241588	0.0
21	4.470044888190767E-5	0.0	0.0	12.061656223151793	0.0
22	4.470044888190767E-5	0.0	0.0	12.505129376509199	0.0
23	4.470044888190767E-5	0.0	0.0	12.986374409171816	0.0
24	4.470044888190767E-5	0.0	0.0	13.329048050300521	0.0
25	4.470044888190767E-5	0.0	0.0	13.630552578008988	0.0
26	4.470044888190767E-5	0.0	0.0	13.908008264218989	0.0
27	4.470044888190767E-5	0.0	0.0	14.21241832110478	0.0
28	4.470044888190767E-5	0.0	0.0	14.519823308065659	0.0
29	4.470044888190767E-5	0.0	0.0	14.865268377025043	0.0
30	4.470044888190767E-5	0.0	0.0	15.252597766586772	0.0
31	4.470044888190767E-5	4.470044888190767E-5	0.0	15.584498599534937	0.0
32	4.470044888190767E-5	4.470044888190767E-5	0.0	15.892395291433516	0.0
33	4.470044888190767E-5	4.470044888190767E-5	0.0	16.205611336749044	0.0
34	4.470044888190767E-5	4.470044888190767E-5	0.0	16.536215856679632	0.0
35	4.470044888190767E-5	4.470044888190767E-5	0.0	16.898110690827558	0.0
36	4.470044888190767E-5	4.470044888190767E-5	0.0	17.217897702128724	0.0
37	4.470044888190767E-5	4.470044888190767E-5	0.0	17.54510498794429	0.0
38	4.470044888190767E-5	4.470044888190767E-5	0.0	17.904496596954825	0.0
39	4.470044888190767E-5	4.470044888190767E-5	0.0	18.43580613236518	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTATC	20	7.0347206E-4	45.0	26
TATCGTG	20	7.0347206E-4	45.0	1
AATCGCG	45	3.8562575E-10	45.0	1
GTAGCGT	25	3.891995E-5	44.999996	10
TCGACCG	25	3.891995E-5	44.999996	19
CGACGGT	830	0.0	44.18675	28
CGTTTTT	9090	0.0	42.99505	1
CGGTCTA	860	0.0	42.645348	31
TATAGCG	95	0.0	42.63158	1
TCACGAC	885	0.0	42.203392	25
GACGGTC	875	0.0	42.17143	29
TACGGGT	395	0.0	42.151897	4
ACGGTCT	875	0.0	41.914288	30
CACGACG	880	0.0	41.676136	26
GCTAGCG	60	3.6379788E-12	41.250004	1
GACGGGT	530	0.0	40.75472	4
TAGGGCG	980	0.0	40.637756	5
CTCACGA	920	0.0	40.597824	24
AAGGGTC	1265	0.0	40.55336	5
CTCGCAG	50	1.0822987E-9	40.499996	1
>>END_MODULE