FastQCFastQC Report
Thu 26 May 2016
SRR1547921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547921_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1426779
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121810.8537411890699261No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27400.19204095378471367No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT21790.1527216198163836TruSeq Adapter, Index 21 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19320.13540989880002438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG351.2122109E-745.0000041
TAACGAC302.1658707E-645.00000422
GCGATCG302.1658707E-645.0000049
TGATCGC207.033859E-445.028
CTATGCG453.8562575E-1045.01
CTCACGG406.8175723E-945.02
GACCGTT207.033859E-445.023
GCTATCG207.033859E-445.041
CCTCGCG453.8562575E-1045.020
TAGTTCG207.033859E-445.01
CCCGTAG406.8175723E-945.043
CTAAACG551.8189894E-1245.01
CTACGCT207.033859E-445.040
TGTTACG207.033859E-445.01
GCGGCCC207.033859E-445.038
CGCCGTT207.033859E-445.026
GTGACCG207.033859E-445.09
CGATCGT207.033859E-445.010
TCGACTG207.033859E-445.01
GCGTCGC207.033859E-445.09