##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547921_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1426779
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.339145025263196	31.0	31.0	33.0	30.0	34.0
2	31.713348037783007	31.0	31.0	34.0	30.0	34.0
3	31.74518758686524	31.0	31.0	34.0	30.0	34.0
4	35.54704968323756	37.0	35.0	37.0	33.0	37.0
5	35.456092359082945	37.0	35.0	37.0	33.0	37.0
6	35.520306228224555	37.0	35.0	37.0	33.0	37.0
7	35.923911131296435	37.0	35.0	37.0	35.0	37.0
8	35.935570960884625	37.0	35.0	37.0	35.0	37.0
9	37.70268626045099	39.0	37.0	39.0	35.0	39.0
10	37.17211495263107	39.0	37.0	39.0	33.0	39.0
11	36.79533340482303	39.0	35.0	39.0	32.0	39.0
12	36.07745418176186	37.0	35.0	39.0	32.0	39.0
13	35.8025833012681	37.0	35.0	39.0	31.0	39.0
14	36.83774571955433	39.0	35.0	41.0	31.0	41.0
15	37.090273265866685	39.0	35.0	41.0	32.0	41.0
16	37.21845289284465	39.0	35.0	41.0	32.0	41.0
17	37.16241618358554	38.0	35.0	41.0	32.0	41.0
18	37.07036899197423	38.0	35.0	41.0	32.0	41.0
19	36.99101052090057	38.0	35.0	41.0	32.0	41.0
20	36.80764715488523	38.0	35.0	40.0	32.0	41.0
21	36.667199335005634	38.0	35.0	40.0	32.0	41.0
22	36.55591440580496	38.0	35.0	40.0	31.0	41.0
23	36.44805257156154	38.0	35.0	40.0	31.0	41.0
24	36.37669604052204	38.0	35.0	40.0	31.0	41.0
25	36.37279354405973	38.0	35.0	40.0	31.0	41.0
26	36.25657652656788	38.0	35.0	40.0	31.0	41.0
27	36.17845090234717	37.0	35.0	40.0	31.0	41.0
28	36.14773836732949	37.0	35.0	40.0	31.0	41.0
29	36.155609943796485	37.0	35.0	40.0	31.0	41.0
30	36.0860287402604	37.0	35.0	40.0	31.0	41.0
31	35.91088038161481	37.0	35.0	40.0	30.0	41.0
32	35.685952764934164	37.0	34.0	40.0	30.0	41.0
33	35.523230297053715	37.0	34.0	40.0	29.0	41.0
34	35.38072259263698	37.0	34.0	40.0	29.0	41.0
35	35.267170318598744	37.0	34.0	40.0	29.0	41.0
36	35.10352128816025	37.0	34.0	40.0	27.0	41.0
37	34.960916862387236	37.0	34.0	40.0	27.0	41.0
38	34.883440252484796	36.0	34.0	40.0	27.0	41.0
39	34.81789260985759	36.0	34.0	40.0	26.0	41.0
40	34.61031315992175	36.0	34.0	40.0	25.0	41.0
41	34.54508371653914	36.0	34.0	40.0	24.0	41.0
42	34.51601264106074	36.0	34.0	40.0	24.0	41.0
43	34.425039897559465	36.0	34.0	40.0	24.0	41.0
44	34.2632503001516	35.0	34.0	40.0	24.0	41.0
45	34.11661091171092	35.0	33.0	40.0	23.0	41.0
46	34.03380691753944	35.0	33.0	40.0	23.0	41.0
47	33.96381429779945	35.0	33.0	40.0	23.0	41.0
48	33.841759655840185	35.0	33.0	40.0	23.0	41.0
49	33.70241992628151	35.0	33.0	40.0	23.0	41.0
50	33.54677634027414	35.0	33.0	39.0	22.0	41.0
51	33.19825565136577	35.0	33.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	9.0
14	19.0
15	50.0
16	108.0
17	237.0
18	536.0
19	1055.0
20	1900.0
21	3286.0
22	5108.0
23	7983.0
24	12107.0
25	17608.0
26	22187.0
27	25150.0
28	27378.0
29	31759.0
30	37778.0
31	45948.0
32	57109.0
33	75986.0
34	132400.0
35	209377.0
36	114068.0
37	129338.0
38	179225.0
39	289025.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.47418065446716	25.308334367130435	30.51853160160053	12.698953376801873
2	30.376393260624106	27.770103148420322	29.264798542731562	12.588705048224005
3	28.257775030330556	25.776942329540876	33.14760029408899	12.817682346039577
4	24.65882943328995	29.99455416711348	31.42925428535183	13.917362114244744
5	23.337040985324286	33.27740315774202	29.991049770146606	13.394506086787091
6	20.893284804444136	41.71788342833754	27.12571463415147	10.263117133066858
7	86.4805972053135	4.331224387238668	7.293210791580195	1.894967615867629
8	86.11452789815381	3.8624061610102194	6.87226262791925	3.150803312916717
9	82.06596817026323	6.2493911110270055	8.824912617861632	2.8597281008481343
10	47.03384336326789	31.006343659389437	12.21044043961959	9.749372537723081
11	38.00392352284411	24.53470369272326	23.834104651105743	13.627268133326886
12	34.84597124011497	25.9893788736728	24.327103216405625	14.837546669806606
13	20.373792998074684	40.16964084837245	25.735940885028448	13.720625268524417
14	15.001342184038313	44.074310036803176	25.956157190426826	14.96819058873168
15	13.086049065762811	30.83764198940411	41.42050030172858	14.655808643104503
16	15.452287985735701	27.902709529646845	40.66320011718704	15.981802367430415
17	15.872745533821286	28.31987294458357	26.157099312507405	29.65028220908774
18	18.44630457835446	30.75388690189581	31.94124668221217	18.858561837537557
19	24.001544738182996	32.041682699282795	24.876873012568872	19.079899549965344
20	26.24597081958734	31.35979713746838	24.342102035423846	18.052130007520436
21	20.01340081400133	35.075088713809215	27.04287068985456	17.868639782334895
22	21.322433257007567	30.615883749340295	24.387869459811228	23.67381353384091
23	17.40241480986193	35.378849842897885	23.850785580668063	23.367949766572117
24	17.719772999182073	31.78782418300241	34.147544924616916	16.344857893198597
25	15.63360548480178	33.107159553091265	31.466541069079373	19.792693893027582
26	15.340147282795725	39.98278640209871	25.59800782041227	19.079058494693292
27	16.603131949657236	40.157095107231044	26.629702287460077	16.610070655651647
28	14.13799894727915	36.087438909599875	33.44757667445344	16.326985468667537
29	14.470846571192874	31.229784009997342	33.11346746763164	21.185901951178142
30	17.61583258514458	36.62977938419335	29.058810089018692	16.69557794164338
31	24.16407866950663	35.19528952977301	23.56720977810859	17.07342202261177
32	24.467559446837946	34.77076688120585	25.491684416437305	15.269989255518897
33	23.845178545521065	35.78423848402591	22.490729117824134	17.879853852628894
34	18.662876310907293	34.893490862985786	26.31164321874656	20.131989607360353
35	17.551281593014757	34.57290862845612	27.30254650509995	20.57326327342917
36	26.251507766794997	32.257413376563576	24.932102308766808	16.558976547874618
37	18.55515114814558	36.64491837909025	27.644155121430856	17.155775351333318
38	18.614025017189068	38.00392352284411	23.277676500705436	20.104374959261385
39	19.878481530776664	35.8117129562462	25.355924077940596	18.95388143503654
40	22.776828086199753	32.592573902475436	23.5279605320796	21.10263747924521
41	17.269738340696072	31.414886257787643	25.841353145792024	25.474022255724254
42	20.12287817524648	32.19061957037495	23.971617188085894	23.714885066292677
43	19.94022900533299	31.995004131684023	25.799230294250197	22.265536568732788
44	18.750766586836505	35.557574088208476	24.833488578118963	20.858170746836056
45	17.283545664745557	40.08721743171157	21.6723122501803	20.956924653362574
46	22.069290338587827	35.85096220227519	24.572761443783513	17.506986015353466
47	20.203969921059954	33.556913859819915	25.19983823703601	21.03927798208412
48	20.67040515735093	30.645811299437405	28.105333762271524	20.578449780940144
49	20.85782030713937	28.46684735337428	28.426476700315884	22.24885563917047
50	18.982126874589547	34.57963707063252	26.151492277360404	20.286743777417527
51	17.50130889226713	35.46078264398341	23.6038657703821	23.434042693367367
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2392.0
1	2535.5
2	2679.0
3	9063.0
4	15447.0
5	11138.5
6	6830.0
7	7368.5
8	7907.0
9	9997.0
10	12087.0
11	14208.0
12	16329.0
13	16829.0
14	17329.0
15	16446.0
16	15563.0
17	14259.0
18	12955.0
19	12203.5
20	11452.0
21	10482.0
22	9512.0
23	9455.5
24	9399.0
25	9758.0
26	11732.5
27	13348.0
28	15918.0
29	18488.0
30	21089.5
31	23691.0
32	27431.0
33	31171.0
34	35082.0
35	38993.0
36	44097.0
37	49201.0
38	57976.5
39	66752.0
40	84781.5
41	102811.0
42	127391.0
43	151971.0
44	161604.5
45	171238.0
46	169141.5
47	167045.0
48	150639.0
49	134233.0
50	115567.5
51	96902.0
52	81720.0
53	66538.0
54	56486.5
55	46435.0
56	39986.5
57	33538.0
58	28638.0
59	23738.0
60	20211.5
61	16685.0
62	13541.5
63	10398.0
64	8086.5
65	5775.0
66	4525.5
67	3276.0
68	2502.5
69	1729.0
70	1381.0
71	1033.0
72	795.5
73	558.0
74	501.5
75	345.5
76	246.0
77	221.5
78	197.0
79	166.5
80	136.0
81	111.0
82	86.0
83	58.0
84	30.0
85	28.5
86	27.0
87	21.5
88	16.0
89	14.5
90	13.0
91	12.5
92	12.0
93	11.5
94	11.0
95	13.0
96	15.0
97	7.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1426779.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.810002254611383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.65746245653894	21.04034201494524
2	9.94031800806678	5.528805324317825
3	3.9071691016260623	3.259751445761062
4	2.0286070382809647	2.2566226523325663
5	1.266447104071283	1.7609948409784222
6	0.8456103781046156	1.4109855912967286
7	0.646270683014403	1.2580952406203847
8	0.47930641426681686	1.0663609969127898
9	0.38042608349919893	0.952168521584313
>10	3.320357929068294	20.727158290383958
>50	1.0887889313741275	21.945411347697995
>100	0.43285533938358994	16.282419556992927
>500	0.0040835409312390885	0.7339736902372644
>1k	0.0020417704656195443	0.9123390208209842
>5k	0.0	0.0
>10k+	2.5522130820244303E-4	0.8645714651175048
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12181	0.8537411890699261	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2740	0.19204095378471367	No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	2179	0.1527216198163836	TruSeq Adapter, Index 21 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1932	0.13540989880002438	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1840509287002402	0.0
2	0.0	0.0	0.0	0.5323178992682118	0.0
3	0.0	0.0	0.0	0.811548249588759	0.0
4	0.0	0.0	0.0	1.310784641489677	0.0
5	0.0	0.0	0.0	2.216881521244706	0.0
6	0.0	0.0	0.0	3.254533463136197	0.0
7	0.0	0.0	0.0	3.8858856206882777	0.0
8	0.0	0.0	0.0	5.272295148723103	0.0
9	0.0	0.0	0.0	5.8167382614967	0.0
10	0.0	0.0	0.0	6.680712289709899	0.0
11	0.0	0.0	0.0	7.9538597077753455	0.0
12	7.008793933748675E-5	0.0	0.0	8.847691198146315	0.0
13	7.008793933748675E-5	0.0	0.0	9.218596573120294	0.0
14	7.008793933748675E-5	0.0	0.0	9.372790039662766	0.0
15	7.008793933748675E-5	0.0	0.0	9.59097379482036	0.0
16	7.008793933748675E-5	0.0	0.0	10.059301405473448	0.0
17	7.008793933748675E-5	0.0	0.0	10.66016530941372	0.0
18	7.008793933748675E-5	0.0	0.0	11.389991021734971	0.0
19	7.008793933748675E-5	0.0	0.0	11.818158243147677	0.0
20	7.008793933748675E-5	0.0	0.0	12.227822248575288	0.0
21	7.008793933748675E-5	0.0	0.0	12.723273891751981	0.0
22	7.008793933748675E-5	0.0	0.0	13.280683273303014	0.0
23	7.008793933748675E-5	0.0	0.0	13.856806134657154	0.0
24	7.008793933748675E-5	0.0	0.0	14.304948418781045	0.0
25	7.008793933748675E-5	0.0	0.0	14.647328002444667	0.0
26	7.008793933748675E-5	0.0	0.0	14.989287058472264	0.0
27	7.008793933748675E-5	0.0	0.0	15.313794217604828	0.0
28	7.008793933748675E-5	0.0	0.0	15.654071163088327	0.0
29	7.008793933748675E-5	0.0	0.0	16.02932199030123	0.0
30	7.008793933748675E-5	0.0	0.0	16.511737276761153	0.0
31	7.008793933748675E-5	0.0	0.0	16.93794203587241	0.0
32	7.008793933748675E-5	0.0	0.0	17.322864998713886	0.0
33	7.008793933748675E-5	0.0	0.0	17.704143388709813	0.0
34	7.008793933748675E-5	0.0	0.0	18.105957544931627	0.0
35	7.008793933748675E-5	0.0	0.0	18.563982228502102	0.0
36	7.008793933748675E-5	0.0	0.0	18.99593419863903	0.0
37	7.008793933748675E-5	0.0	0.0	19.41456946030184	0.0
38	7.008793933748675E-5	0.0	0.0	19.822411179306677	0.0
39	7.008793933748675E-5	0.0	0.0	20.241326792726834	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAGCG	35	1.2122109E-7	45.000004	1
TAACGAC	30	2.1658707E-6	45.000004	22
GCGATCG	30	2.1658707E-6	45.000004	9
TGATCGC	20	7.033859E-4	45.0	28
CTATGCG	45	3.8562575E-10	45.0	1
CTCACGG	40	6.8175723E-9	45.0	2
GACCGTT	20	7.033859E-4	45.0	23
GCTATCG	20	7.033859E-4	45.0	41
CCTCGCG	45	3.8562575E-10	45.0	20
TAGTTCG	20	7.033859E-4	45.0	1
CCCGTAG	40	6.8175723E-9	45.0	43
CTAAACG	55	1.8189894E-12	45.0	1
CTACGCT	20	7.033859E-4	45.0	40
TGTTACG	20	7.033859E-4	45.0	1
GCGGCCC	20	7.033859E-4	45.0	38
CGCCGTT	20	7.033859E-4	45.0	26
GTGACCG	20	7.033859E-4	45.0	9
CGATCGT	20	7.033859E-4	45.0	10
TCGACTG	20	7.033859E-4	45.0	1
GCGTCGC	20	7.033859E-4	45.0	9
>>END_MODULE