FastQCFastQC Report
Thu 26 May 2016
SRR1547920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547920_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2669183
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236640.8865634165960146No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC50810.1903578735515699No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT27720.10385200265399562Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC207.034964E-445.00000429
GCGCGTC1700.043.67646838
CGGTCTA9700.043.60824631
CGACGGT9500.043.57894528
CTCACGA9900.043.4090924
CGTTTTT116450.043.0871621
CGCATCG2300.043.04347621
TGCGCGT1750.042.4285737
TCACGAC10100.042.32673325
CACGACG9900.042.27272826
GCGATAC750.042.0000049
GACGGTC9900.041.8181829
TCTCACG10450.041.55502323
TACGGGA11900.041.029414
ACTAACC11300.041.017714
CTAAGCG556.184564E-1140.9090921
ACGGTCT10150.040.78817730
ACGACGG10350.040.65217627
TAGTACG950.040.2631531
GCGATCG451.9299478E-840.09
Produced by FastQC (version 0.11.5)