##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547920_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2669183
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.318987870071105	31.0	31.0	33.0	30.0	34.0
2	31.704239462037634	31.0	31.0	34.0	30.0	34.0
3	31.740257599422744	31.0	31.0	34.0	30.0	34.0
4	35.56681988458641	37.0	35.0	37.0	33.0	37.0
5	35.48137913361504	37.0	35.0	37.0	33.0	37.0
6	35.55892158761689	37.0	35.0	37.0	33.0	37.0
7	35.919550289358206	37.0	35.0	37.0	35.0	37.0
8	35.940280602716264	37.0	35.0	37.0	35.0	37.0
9	37.70239994784921	39.0	37.0	39.0	35.0	39.0
10	37.14480723127639	39.0	37.0	39.0	33.0	39.0
11	36.83902714800746	39.0	37.0	39.0	32.0	39.0
12	36.423160944753505	38.0	35.0	39.0	32.0	39.0
13	36.28197654488284	39.0	35.0	39.0	32.0	39.0
14	37.344191087684884	39.0	35.0	41.0	32.0	41.0
15	37.524814147250304	39.0	35.0	41.0	32.0	41.0
16	37.6025810144902	40.0	35.0	41.0	33.0	41.0
17	37.537055720795465	39.0	35.0	41.0	32.0	41.0
18	37.41896902535345	39.0	36.0	41.0	32.0	41.0
19	37.347499590698725	39.0	36.0	41.0	32.0	41.0
20	37.1821448735437	39.0	35.0	41.0	32.0	41.0
21	37.06728126171941	39.0	35.0	41.0	32.0	41.0
22	36.97798165206357	39.0	35.0	41.0	32.0	41.0
23	36.85186740661843	38.0	35.0	41.0	31.0	41.0
24	36.80445589530579	38.0	35.0	41.0	31.0	41.0
25	36.81922595790547	38.0	35.0	41.0	31.0	41.0
26	36.71027351815143	38.0	35.0	41.0	31.0	41.0
27	36.63384751064277	38.0	35.0	41.0	31.0	41.0
28	36.57233842715168	38.0	35.0	41.0	31.0	41.0
29	36.564697886956424	38.0	35.0	40.0	31.0	41.0
30	36.46451667045684	38.0	35.0	40.0	31.0	41.0
31	36.309945777415784	38.0	35.0	40.0	30.0	41.0
32	36.14141293421995	38.0	35.0	40.0	30.0	41.0
33	35.959323133707954	38.0	35.0	40.0	30.0	41.0
34	35.76337291223569	38.0	35.0	40.0	29.0	41.0
35	35.62760702432168	38.0	35.0	40.0	28.0	41.0
36	35.52744341620638	38.0	35.0	40.0	27.0	41.0
37	35.39977850900444	38.0	34.0	40.0	27.0	41.0
38	35.34038805132507	38.0	34.0	40.0	27.0	41.0
39	35.25560967532012	38.0	34.0	40.0	26.0	41.0
40	35.1098714475553	38.0	34.0	40.0	25.0	41.0
41	35.01767769388611	38.0	34.0	40.0	25.0	41.0
42	35.024934221445285	38.0	34.0	40.0	25.0	41.0
43	34.99055029198073	38.0	34.0	40.0	25.0	41.0
44	34.84754511024534	38.0	34.0	40.0	24.0	41.0
45	34.7362646173005	37.0	34.0	40.0	24.0	41.0
46	34.67439399996179	37.0	34.0	40.0	24.0	41.0
47	34.615058615314126	37.0	34.0	40.0	24.0	41.0
48	34.48256451505948	37.0	33.0	40.0	23.0	41.0
49	34.35899674169961	37.0	33.0	40.0	24.0	41.0
50	34.25725324940253	37.0	33.0	40.0	23.0	41.0
51	33.96296919319507	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	0.0
13	10.0
14	30.0
15	70.0
16	154.0
17	388.0
18	954.0
19	1910.0
20	3322.0
21	5589.0
22	8915.0
23	13744.0
24	20882.0
25	31100.0
26	41308.0
27	46972.0
28	50485.0
29	56639.0
30	65393.0
31	79634.0
32	98070.0
33	125311.0
34	202040.0
35	286481.0
36	206398.0
37	257181.0
38	394460.0
39	671657.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.833585782615877	25.452807094905072	29.090362107056727	15.623245015422322
2	30.576734528880184	26.368443077900615	30.090818051815855	12.964004341403342
3	27.86612982324554	26.21566224571339	33.27377703214804	12.644430898893033
4	25.335355425236862	28.018198827131748	32.27650558241979	14.369940165211602
5	22.581291728592607	32.47349469856506	30.56171120526393	14.383502367578394
6	21.325364353062344	39.96350194048141	27.95746863366056	10.753665072795684
7	88.01108803705104	2.7606574745905395	7.539722829045442	1.6885316593129807
8	88.41686763327955	2.1852379548348693	7.291781792406141	2.1061126194794437
9	83.56853014574122	5.199306304588333	8.668982231641667	2.563181318028775
10	38.5710533897451	38.90767324683246	13.170022437577341	9.351250925845099
11	29.97497736198679	24.094451373322848	29.58474559443845	16.34582567025191
12	29.555335846212117	23.489322388161472	29.521355411000293	17.433986354626114
13	22.102381140596204	30.67444232935696	30.752443725289723	16.47073280475711
14	16.67585174939298	34.26550371405782	30.949507770729845	18.109136765819354
15	15.321504745084919	27.65557850473347	40.10429408549358	16.918622664688034
16	19.164815600878622	25.867840459046832	37.93475381792856	17.03259012214599
17	18.88405553309758	25.504433379052692	30.339883027877818	25.271628059971906
18	19.376341000223665	27.541311330096136	33.57896405004827	19.503383619631926
19	22.787085036882072	28.248006974418765	29.317997304793263	19.6469106839059
20	23.98010926939067	28.59594115502759	28.541917133444954	18.88203244213679
21	21.486162619797895	28.32331091573714	31.96573633205367	18.224790132411304
22	21.248411967257397	24.960821344958365	30.704076865467822	23.086689822316416
23	17.65705086537716	30.069350808843005	30.392445928210993	21.881152397568844
24	18.178071717075976	28.13621246651129	34.82781060721577	18.85790520919697
25	18.472955956935134	29.245278424147013	31.84794748055866	20.433818138359193
26	17.708976866704155	32.83158179862527	28.995089508662385	20.464351826008183
27	18.068450158718978	31.69374299176939	31.057705672484804	19.180101177026827
28	16.349309882462162	29.32459108273955	34.81690839481594	19.509190639982346
29	17.428216798923117	27.49908867245146	33.6974647298443	21.375229798781127
30	18.983936283124837	29.487449905083317	31.736789871657358	19.79182394013449
31	22.637563629020566	29.034277529865882	28.17880977063019	20.149349070483368
32	23.76082868802926	29.01442126673218	28.159478012560395	19.065272032678163
33	23.18889338048384	28.89460932427638	27.81869957960919	20.097797715630588
34	19.49154479104655	28.66378963150897	30.34299259361385	21.501672983830634
35	19.898036215576077	27.883850601476183	30.47213323327775	21.745979949669994
36	24.392107997091244	27.36961085096076	28.941440133553975	19.29684101839402
37	19.318795301783354	30.214301529719016	31.14964391725858	19.31725925123905
38	20.681871568940757	30.692387895472134	28.10283146565822	20.52290906992889
39	20.08063890711128	29.690845475937767	29.757907194823286	20.470608422127672
40	22.133064686834885	27.684276424658783	29.27678619262898	20.905872695877353
41	18.54410132238966	27.042169832491815	30.560437407251584	23.853291437866943
42	20.70195261995899	28.351484330598538	28.930987496923215	22.015575552519255
43	20.662652204813234	28.390784745744295	29.555260916917277	21.391302132525198
44	19.992522056374554	29.873710420004922	29.000971458307657	21.13279606531287
45	19.126863913040058	31.82438221733017	26.974845861074343	22.073908008555428
46	21.757781313607946	30.037318535297132	28.149325093109017	20.05557505798591
47	20.517064584931045	28.35676684588505	29.647461414223002	21.4787071549609
48	20.369416409440642	28.386626169880447	30.56527034676903	20.67868707390988
49	20.700528963356952	26.35394425934827	30.67406768288274	22.271459094412034
50	19.910699266404738	29.517646410905506	29.735540800312304	20.83611352237745
51	19.0071268998791	29.48576399594932	28.294275813985024	23.212833290186545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2209.0
1	3196.0
2	4183.0
3	19218.0
4	34253.0
5	24612.0
6	14971.0
7	14510.0
8	14049.0
9	15620.0
10	17191.0
11	19080.0
12	20969.0
13	20839.0
14	20709.0
15	19899.0
16	19089.0
17	17810.5
18	16532.0
19	16093.5
20	15655.0
21	15675.5
22	15696.0
23	16400.0
24	17104.0
25	21606.0
26	29623.0
27	33138.0
28	40350.0
29	47562.0
30	54311.0
31	61060.0
32	66668.5
33	72277.0
34	81820.5
35	91364.0
36	97731.5
37	104099.0
38	118498.0
39	132897.0
40	152292.0
41	171687.0
42	188257.0
43	204827.0
44	208511.5
45	212196.0
46	216078.5
47	219961.0
48	228498.0
49	237035.0
50	232508.5
51	227982.0
52	206940.0
53	185898.0
54	158657.0
55	131416.0
56	113626.0
57	95836.0
58	82935.5
59	70035.0
60	59448.0
61	48861.0
62	40824.5
63	32788.0
64	27202.0
65	21616.0
66	16311.0
67	11006.0
68	8570.0
69	6134.0
70	5215.0
71	4296.0
72	3388.0
73	2480.0
74	2054.5
75	1217.5
76	806.0
77	765.0
78	724.0
79	529.0
80	334.0
81	288.0
82	242.0
83	181.0
84	120.0
85	96.0
86	72.0
87	56.5
88	41.0
89	33.5
90	26.0
91	19.0
92	12.0
93	10.0
94	8.0
95	4.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2669183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.535997610308844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.69257576818737	17.058077066299916
2	9.140935515235258	4.120002018546581
3	3.487932164904253	2.358120927996048
4	1.8935673317914417	1.706937154568433
5	1.1855072130578752	1.3358293860238089
6	0.8148516578895834	1.1018097008973513
7	0.5942885831211489	0.937502026233643
8	0.449017233326673	0.8095241037789913
9	0.3687428571643695	0.7478989333087218
>10	4.146677127652601	22.11395496410579
>50	1.5695836397649086	25.390252697057047
>100	0.6499294054022801	19.879427922801963
>500	0.004204935856767627	0.633998524337007
>1k	0.0018501717769777559	0.7170862180681273
>5k	1.681974342707051E-4	0.19259515139043004
>10k+	1.681974342707051E-4	0.8969832045863287
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23664	0.8865634165960146	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5081	0.1903578735515699	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	2772	0.10385200265399562	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.492929484415269E-5	0.0	0.0	0.12947782149069584	0.0
2	7.492929484415269E-5	0.0	0.0	0.3859233330948084	0.0
3	7.492929484415269E-5	0.0	0.0	0.5954256414790593	0.0
4	7.492929484415269E-5	0.0	0.0	0.8817304770785668	0.0
5	7.492929484415269E-5	0.0	0.0	1.5681577471458494	0.0
6	7.492929484415269E-5	0.0	0.0	2.34873367618481	0.0
7	7.492929484415269E-5	0.0	0.0	2.8440537797520813	0.0
8	7.492929484415269E-5	0.0	0.0	3.7278448124388626	0.0
9	7.492929484415269E-5	0.0	0.0	4.125382186234514	0.0
10	7.492929484415269E-5	0.0	0.0	4.7151881306002625	0.0
11	7.492929484415269E-5	0.0	0.0	5.469613735738614	0.0
12	7.492929484415269E-5	0.0	0.0	6.072157660227868	0.0
13	7.492929484415269E-5	0.0	0.0	6.329727111254642	0.0
14	7.492929484415269E-5	0.0	0.0	6.443132599001268	0.0
15	7.492929484415269E-5	0.0	0.0	6.5856855824422675	0.0
16	7.492929484415269E-5	0.0	0.0	6.851272468017367	0.0
17	7.492929484415269E-5	0.0	0.0	7.191826113084041	0.0
18	7.492929484415269E-5	0.0	0.0	7.581945486690122	0.0
19	7.492929484415269E-5	0.0	0.0	7.814938129008015	0.0
20	7.492929484415269E-5	0.0	0.0	8.04669443796098	0.0
21	7.492929484415269E-5	0.0	0.0	8.34495049608813	0.0
22	7.492929484415269E-5	0.0	0.0	8.66126451427272	0.0
23	7.492929484415269E-5	3.7464647422076344E-5	0.0	9.000919007801263	0.0
24	7.492929484415269E-5	3.7464647422076344E-5	0.0	9.255603680976538	0.0
25	7.492929484415269E-5	3.7464647422076344E-5	0.0	9.48099099986775	0.0
26	7.492929484415269E-5	3.7464647422076344E-5	0.0	9.690568237546845	0.0
27	7.492929484415269E-5	3.7464647422076344E-5	0.0	9.903742081378459	0.0
28	7.492929484415269E-5	3.7464647422076344E-5	0.0	10.128342642673807	0.0
29	7.492929484415269E-5	3.7464647422076344E-5	0.0	10.378231840979057	0.0
30	7.492929484415269E-5	3.7464647422076344E-5	0.0	10.689975172178153	0.0
31	7.492929484415269E-5	3.7464647422076344E-5	0.0	10.96852482576129	0.0
32	7.492929484415269E-5	3.7464647422076344E-5	0.0	11.218301630124275	0.0
33	7.492929484415269E-5	3.7464647422076344E-5	0.0	11.47407277807479	0.0
34	7.492929484415269E-5	3.7464647422076344E-5	0.0	11.736587562561278	0.0
35	7.492929484415269E-5	3.7464647422076344E-5	0.0	12.036904176296643	0.0
36	7.492929484415269E-5	3.7464647422076344E-5	0.0	12.311969617669527	0.0
37	7.492929484415269E-5	3.7464647422076344E-5	0.0	12.598499241153567	0.0
38	7.492929484415269E-5	3.7464647422076344E-5	0.0	12.883792531272677	0.0
39	7.492929484415269E-5	3.7464647422076344E-5	0.0	13.228167570376403	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCGC	20	7.034964E-4	45.000004	29
GCGCGTC	170	0.0	43.676468	38
CGGTCTA	970	0.0	43.608246	31
CGACGGT	950	0.0	43.578945	28
CTCACGA	990	0.0	43.40909	24
CGTTTTT	11645	0.0	43.087162	1
CGCATCG	230	0.0	43.043476	21
TGCGCGT	175	0.0	42.42857	37
TCACGAC	1010	0.0	42.326733	25
CACGACG	990	0.0	42.272728	26
GCGATAC	75	0.0	42.000004	9
GACGGTC	990	0.0	41.81818	29
TCTCACG	1045	0.0	41.555023	23
TACGGGA	1190	0.0	41.02941	4
ACTAACC	1130	0.0	41.0177	14
CTAAGCG	55	6.184564E-11	40.909092	1
ACGGTCT	1015	0.0	40.788177	30
ACGACGG	1035	0.0	40.652176	27
TAGTACG	95	0.0	40.263153	1
GCGATCG	45	1.9299478E-8	40.0	9
>>END_MODULE