FastQCFastQC Report
Thu 26 May 2016
SRR1547919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547919_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3759974
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192970.5132216339793839No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC40710.10827202528528124No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT10500.042.6428628
CGGTCTA10650.042.04225531
CGTTTTT110150.041.6295971
CACGACG11100.040.74324426
TATAGCG2400.040.3124961
TACGGGA18800.039.853724
GACGGTC11300.039.42477829
CTCACGA11600.039.37524
TAAGGGA70650.039.1719744
TTAGGGA68650.038.838314
TATGGGA58050.038.837214
CGAGGGA31450.038.7758374
TCACGAC11850.038.54430425
ACGGTCT11700.038.2692330
AACGGGA18950.038.1134574
TAGGGAT76900.037.9778945
AGGGATT86650.037.8332376
ATAGCGG8450.037.810652
TAGGGAC51700.037.5580255
GTAGGGT18200.037.458794
Produced by FastQC (version 0.11.5)