##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547919_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3759974
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.341409009743153	31.0	31.0	33.0	30.0	34.0
2	31.74615968089141	31.0	31.0	34.0	30.0	34.0
3	31.84203800345428	31.0	31.0	34.0	30.0	34.0
4	35.63681211625399	37.0	35.0	37.0	33.0	37.0
5	35.553441061028614	37.0	35.0	37.0	33.0	37.0
6	35.6114361429095	37.0	35.0	37.0	33.0	37.0
7	35.92457022309197	37.0	35.0	37.0	35.0	37.0
8	35.92385106918292	37.0	35.0	37.0	35.0	37.0
9	37.66974399291059	39.0	37.0	39.0	35.0	39.0
10	37.142260824143996	39.0	37.0	39.0	33.0	39.0
11	36.9763391980902	39.0	37.0	39.0	33.0	39.0
12	36.59423389629822	39.0	35.0	39.0	32.0	39.0
13	36.477694792570375	39.0	35.0	39.0	32.0	39.0
14	37.57755000433514	40.0	36.0	41.0	32.0	41.0
15	37.71081369179681	40.0	36.0	41.0	33.0	41.0
16	37.76731647612457	40.0	36.0	41.0	33.0	41.0
17	37.68705927221837	40.0	36.0	41.0	33.0	41.0
18	37.58093274049236	39.0	36.0	41.0	32.0	41.0
19	37.53972208318462	39.0	36.0	41.0	32.0	41.0
20	37.41247120325832	39.0	35.0	41.0	32.0	41.0
21	37.30499546007499	39.0	35.0	41.0	32.0	41.0
22	37.22761965907211	39.0	35.0	41.0	32.0	41.0
23	37.08801390647914	39.0	35.0	41.0	32.0	41.0
24	37.05440197192853	39.0	35.0	41.0	31.0	41.0
25	37.07821171103843	39.0	35.0	41.0	32.0	41.0
26	36.98482782061791	39.0	35.0	41.0	31.0	41.0
27	36.920003170234686	39.0	35.0	41.0	31.0	41.0
28	36.84830214251482	39.0	35.0	41.0	31.0	41.0
29	36.81806310362784	39.0	35.0	41.0	31.0	41.0
30	36.740420811420506	39.0	35.0	41.0	31.0	41.0
31	36.63509641290073	39.0	35.0	41.0	30.0	41.0
32	36.498018603320126	39.0	35.0	41.0	30.0	41.0
33	36.38343403438428	39.0	35.0	41.0	30.0	41.0
34	36.228051576952396	39.0	35.0	41.0	30.0	41.0
35	36.12044577967826	39.0	35.0	41.0	30.0	41.0
36	36.039409049105124	39.0	35.0	41.0	29.0	41.0
37	35.97247215007338	38.0	35.0	41.0	29.0	41.0
38	35.85691310631403	38.0	35.0	40.0	28.0	41.0
39	35.7847740436503	38.0	35.0	40.0	28.0	41.0
40	35.63738153508508	38.0	35.0	40.0	27.0	41.0
41	35.49953882659827	38.0	34.0	40.0	27.0	41.0
42	35.463289107850215	38.0	34.0	40.0	27.0	41.0
43	35.404149603162146	38.0	34.0	40.0	26.0	41.0
44	35.27407902288686	38.0	34.0	40.0	26.0	41.0
45	35.167887863054375	38.0	34.0	40.0	26.0	41.0
46	35.069310851617594	38.0	34.0	40.0	25.0	41.0
47	34.98632277776389	38.0	34.0	40.0	25.0	41.0
48	34.82879323101702	38.0	34.0	40.0	24.0	41.0
49	34.67375226530822	37.0	34.0	40.0	24.0	41.0
50	34.58155774481419	37.0	34.0	40.0	24.0	41.0
51	34.26565449654705	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	4.0
12	6.0
13	8.0
14	22.0
15	65.0
16	204.0
17	529.0
18	1059.0
19	2263.0
20	4138.0
21	6994.0
22	11131.0
23	16785.0
24	25528.0
25	37527.0
26	49976.0
27	58796.0
28	65894.0
29	74723.0
30	88813.0
31	106867.0
32	131709.0
33	168081.0
34	258782.0
35	349640.0
36	287825.0
37	380277.0
38	599656.0
39	1032524.0
40	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.583862016067133	25.11714176746967	29.481214497759822	13.817781718703372
2	31.187822043450304	26.54028990625999	29.06081265455559	13.21107539573412
3	28.602750976469515	26.388905880732157	31.628489984239256	13.379853158559074
4	25.660469992611652	28.624134103054967	31.37630738935961	14.339088514973774
5	22.89265830029676	33.6027323593195	28.89232744694511	14.612281893438626
6	21.89435352478501	41.10400763409534	26.10557945347494	10.896059387644701
7	89.21934034650239	2.84190794936348	6.223181330509201	1.715570373624924
8	89.30689414341695	2.3790590041314115	5.986185010853799	2.3278618415978407
9	83.17169214467972	6.051770570753947	8.002954275747651	2.773583008818678
10	31.689926579279536	45.529676534997314	12.442745614730315	10.337651270992831
11	30.591222173344818	23.321916587721088	28.76929468129301	17.31756655764109
12	29.459831371174378	21.752544033549167	29.48578899747711	19.301835597799347
13	24.470248996402635	26.606939303303694	30.821436531210054	18.101375169083617
14	19.063855228786156	30.606195681140345	30.83420257693271	19.495746513140784
15	17.394854326120342	27.76261218827577	36.97483014510207	17.867703340501823
16	21.61291540845761	26.06390363337619	34.32427990193549	17.998901056230707
17	21.20065191940157	25.765151567537437	29.231877667239186	23.80231884582181
18	20.81562266122053	25.929354830645106	32.71900284416861	20.536019663965764
19	23.59649295447256	27.432663098202276	28.73985298834513	20.230990958980037
20	24.961528989296202	27.204682798338496	28.265168854891016	19.568619357474283
21	23.493407135262107	26.878430542338855	31.06707652765684	18.561085794742198
22	22.87584967342859	23.35569341702895	30.186166180936358	23.582290728606104
23	19.34449546725589	27.916123888090716	29.883078978737622	22.856301665915773
24	19.315319733593903	26.767312752694565	33.27477796389018	20.642589549821356
25	20.312321308604794	28.08370483412917	30.65920136681796	20.94477249044807
26	19.71130651435356	30.201591819517905	28.231046278511503	21.856055387617044
27	19.09058413701797	29.924542031407665	29.827520083915477	21.157353747658895
28	18.258211359972172	28.262668837603666	33.20147958469926	20.277640217724908
29	19.11475983610525	26.05773337794357	32.35072902099855	22.47677776495263
30	20.376231325003843	27.509950866681525	30.663350331677826	21.450467476636806
31	22.72324223518567	27.16401762352612	27.947640063468526	22.165100077819687
32	23.360347704532	27.40135969025318	28.45357441301456	20.784718192200266
33	22.42800083192065	26.6704769767025	28.760385045215735	22.141137146161117
34	19.662476389464395	27.373194601877564	29.728663017350655	23.235665991307386
35	20.028197003489918	26.81499925265441	30.284970055644	22.871833688211673
36	22.90100942187366	25.985259472538907	29.48578899747711	21.62794210811032
37	20.50873224123358	28.253971968955106	30.055340808207713	21.181954981603596
38	19.96266463544695	28.372775981961578	29.031902880179487	22.632656502411987
39	20.094261290104665	27.2440979645072	30.070473891574785	22.591166853813352
40	22.115684842501572	25.754300428673176	29.875765098375677	22.25424963044957
41	19.42585241280924	26.141723320427218	29.871403366087108	24.561020900676443
42	20.88134120076362	26.645237440471664	29.15552075626055	23.317900602504167
43	20.62014258609235	26.064861086805386	29.785977243459662	23.5290190836426
44	20.199474783602227	27.654499738562023	29.18695714385259	22.95906833398316
45	19.673566891685955	28.76437443450407	28.035778970812032	23.526279702997947
46	21.518154114895474	27.814926379810075	28.407324093198515	22.259595412095933
47	20.696073962213568	27.185294366397216	29.223898888662532	22.89473278272669
48	20.391231428727966	26.246830430210423	30.57683377597824	22.785104365083374
49	21.00519844020198	24.92424149741461	30.779441559968234	23.291118502415177
50	19.848701081443647	26.959494932677725	30.55587618425021	22.635927801628416
51	19.44883129511002	27.269390692595213	29.01719533166985	24.26458268062492
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2130.0
1	3667.5
2	5205.0
3	18929.0
4	32653.0
5	24212.5
6	15772.0
7	15098.5
8	14425.0
9	14660.5
10	14896.0
11	15333.5
12	15771.0
13	15383.5
14	14996.0
15	14816.5
16	14637.0
17	14318.0
18	13999.0
19	14272.5
20	14546.0
21	16760.0
22	18974.0
23	21160.0
24	23346.0
25	28568.0
26	39859.5
27	45929.0
28	52142.5
29	58356.0
30	69502.0
31	80648.0
32	92565.5
33	104483.0
34	117739.5
35	130996.0
36	141778.0
37	152560.0
38	168955.5
39	185351.0
40	200696.5
41	216042.0
42	239911.5
43	263781.0
44	281089.0
45	298397.0
46	318085.5
47	337774.0
48	343283.5
49	348793.0
50	322143.0
51	295493.0
52	264455.5
53	233418.0
54	205651.5
55	177885.0
56	166007.5
57	154130.0
58	141040.5
59	127951.0
60	114383.5
61	100816.0
62	87107.5
63	73399.0
64	62774.0
65	52149.0
66	43001.5
67	33854.0
68	26626.5
69	19399.0
70	16599.0
71	13799.0
72	11112.5
73	8426.0
74	6774.5
75	4076.0
76	3029.0
77	2190.0
78	1351.0
79	1032.0
80	713.0
81	530.0
82	347.0
83	300.0
84	253.0
85	168.5
86	84.0
87	67.0
88	50.0
89	43.0
90	36.0
91	25.5
92	15.0
93	9.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3759974.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.400879168580172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.1282746214862	16.29212006486915
2	9.255334856492619	3.9614460585709366
3	3.381979940642495	2.171320321807559
4	1.7042864144707646	1.4589291049896635
5	0.9809964612217716	1.049709336570594
6	0.662412178973235	0.8505721801201263
7	0.47710921348283464	0.7147389639573727
8	0.3737603926896283	0.6399040801561449
9	0.2971114314774285	0.5722601260187101
>10	4.009324437491864	21.959245433653773
>50	2.163801964196434	33.21819739441189
>100	0.5598001573184129	15.310200899993934
>500	0.0039140398205620795	0.6162050276819067
>1k	0.0017676308867054292	0.6637342819547554
>5k	0.0	0.0
>10k+	1.262593490503878E-4	0.5214167252434829
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19297	0.5132216339793839	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4071	0.10827202528528124	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.31918571777358E-5	0.0	0.0	0.1140965336462433	0.0
2	5.31918571777358E-5	0.0	0.0	0.33345975264722577	0.0
3	5.31918571777358E-5	0.0	0.0	0.5271313046313618	0.0
4	7.978778576660371E-5	0.0	0.0	0.8200854580377418	0.0
5	7.978778576660371E-5	0.0	0.0	1.4050363114213025	0.0
6	7.978778576660371E-5	0.0	0.0	2.1402541613319666	0.0
7	7.978778576660371E-5	0.0	0.0	2.541028209237617	0.0
8	7.978778576660371E-5	0.0	0.0	3.4223906867441105	0.0
9	7.978778576660371E-5	0.0	0.0	3.759174930464945	0.0
10	7.978778576660371E-5	0.0	0.0	4.335375723342768	0.0
11	7.978778576660371E-5	0.0	0.0	4.979209962622082	0.0
12	7.978778576660371E-5	0.0	0.0	5.554772453213772	0.0
13	7.978778576660371E-5	0.0	0.0	5.799056057302524	0.0
14	7.978778576660371E-5	0.0	0.0	5.911849390447912	0.0
15	7.978778576660371E-5	0.0	0.0	6.068765369122233	0.0
16	7.978778576660371E-5	0.0	0.0	6.368102545389942	0.0
17	7.978778576660371E-5	0.0	0.0	6.7460572865663435	0.0
18	7.978778576660371E-5	0.0	0.0	7.202310441508372	0.0
19	7.978778576660371E-5	0.0	0.0	7.471221875470415	0.0
20	7.978778576660371E-5	0.0	0.0	7.7661973194495495	0.0
21	7.978778576660371E-5	0.0	0.0	8.12279552996909	0.0
22	7.978778576660371E-5	0.0	0.0	8.528011097949081	0.0
23	7.978778576660371E-5	0.0	0.0	8.944157592579097	0.0
24	7.978778576660371E-5	0.0	0.0	9.252457596781253	0.0
25	7.978778576660371E-5	0.0	0.0	9.513017909166393	0.0
26	7.978778576660371E-5	0.0	0.0	9.769110105548602	0.0
27	1.063837143554716E-4	0.0	0.0	10.029191691219141	0.0
28	1.063837143554716E-4	0.0	0.0	10.314167066048862	0.0
29	1.063837143554716E-4	0.0	0.0	10.614568079460124	0.0
30	1.063837143554716E-4	0.0	0.0	10.966964133262623	0.0
31	1.063837143554716E-4	0.0	0.0	11.287338689044127	0.0
32	1.063837143554716E-4	0.0	0.0	11.580319438379096	0.0
33	1.063837143554716E-4	0.0	0.0	11.880614068076003	0.0
34	1.063837143554716E-4	0.0	0.0	12.18489808706124	0.0
35	1.063837143554716E-4	0.0	0.0	12.520831261067231	0.0
36	1.063837143554716E-4	0.0	0.0	12.836684508988625	0.0
37	1.063837143554716E-4	0.0	0.0	13.15820268969945	0.0
38	1.063837143554716E-4	0.0	0.0	13.478018730980587	0.0
39	1.063837143554716E-4	0.0	0.0	13.847117027936894	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	1050	0.0	42.64286	28
CGGTCTA	1065	0.0	42.042255	31
CGTTTTT	11015	0.0	41.629597	1
CACGACG	1110	0.0	40.743244	26
TATAGCG	240	0.0	40.312496	1
TACGGGA	1880	0.0	39.85372	4
GACGGTC	1130	0.0	39.424778	29
CTCACGA	1160	0.0	39.375	24
TAAGGGA	7065	0.0	39.171974	4
TTAGGGA	6865	0.0	38.83831	4
TATGGGA	5805	0.0	38.83721	4
CGAGGGA	3145	0.0	38.775837	4
TCACGAC	1185	0.0	38.544304	25
ACGGTCT	1170	0.0	38.26923	30
AACGGGA	1895	0.0	38.113457	4
TAGGGAT	7690	0.0	37.977894	5
AGGGATT	8665	0.0	37.833237	6
ATAGCGG	845	0.0	37.81065	2
TAGGGAC	5170	0.0	37.558025	5
GTAGGGT	1820	0.0	37.45879	4
>>END_MODULE